| Project/Area Number |
16510071
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | National Institute for Environmental Studies |
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Principal Investigator |
ITAYAMA Tomoaki National Institute for Environmental Studies, Research center for Material Cycles and Waste Management, Researcher, 循環型社会形成推進・廃棄物研究センター, 研究員 (80353530)
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| Co-Investigator(Kenkyū-buntansha) |
INAMORI Yuhei National Institute for Environmental Studies, Research center for Material Cycles and Waste Management, Head of Laboratory, 循環型社会形成推進, 廃棄物研究センター・室長 (10142093)
IWAMI Norio Meisei University, Faculty of Science and Engineering, Associate Professor, 理工学部, 助教授 (00353532)
川端 善一郎 京都大学, 生態学研究センター, 教授 (80108456)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | detritus food web / microbial ecosystem / microcosm / protozoa / rotifer / organic carbon / predator / molecular biology / 腐生食物連鎖 / 輪虫類 / 浄化槽 / 生物学的水処理装置 / 微小後生動物 / ヒルガタワムシ / 水質 |
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| Research Abstract |
In this study, it aimed to clarify the relations between the microbial ecosystems in biological wastewater treatment systems and its purification functions for waste water. Some bacteria were isolated from Johkasou systems, which are typical on-site biological domestic wastewater treatment facilities in Japan, and the isolated bacteria were identified by sequences of 16SrRNA. Some appropriate bacteria (Serratia marcescens etc.) were selected, in order to study on the function of microbial ecosystems for the wastewater treatment process. Moreover, it was shown that the T-RFLP method was a useful molecular biological tool for the analysis on complex microbial ecosystems in wastewater treatment systems.
The mixed culture experiments with aquatic rotatoria, Philodina sp. and isolated bacteria from some Johkasous were performed. It was shown that Philodina sp. was very effective to make high transparent water by the filtration effect and the coagulation effect of Philodina sp.. Moreover, we obtained the result that mineralization of organic carbon under the condition of predation for bacteria by Philodina sp was faster than it under the monoculture condition of bacteria. Also, total amount of decreases of DOC and TOC was enhanced by the existence of Ciliata T.thermophila. in the mixed culture system with bacteria as a microcosm system with a simple detritus food web structure. On the other hand, the numerical analysis of a simple predator-prey model drew out the result of contradiction to our experimental results. That is, our experimental results strongly suggest that existence of predator such as protozoan and/or metazoan increase the activity of bacteria for decomposition of organic matter, because the simple predator prey interaction between bacteria and protozoa only causes decrease of bacterial population as decomposer of organic matter.
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