| Project/Area Number |
16510144
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
基礎ゲノム科学
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
WATANABE Yishihisa Hamamatsu University School of Medicine, assistant professor, 医学部, 助手 (00362187)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIMURA Haruhiko Hamamatsu University School of Medicine, professor, 医学部, 教授 (00196742)
IKEMURA Toshimichi Hayama Institute, professor, 葉山高等研究センター, 教授 (50025475)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2004: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | replication timing / p53 / microarray / genome-wide / genetic instability / chromosome instability / 染色体不安定性 / アンプリコン / replicatino timing / S期内複製時期 / がん抑制遺伝子p53 / ゲノム不安定性 / DNAマイクロアレイ |
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| Research Abstract |
The completion of the human genome sequence will greatly accelerate development of a new branch of biosience and provide fundamental knowledge to biomedical research. We used DNA microarray based on the sequence information to measure replication timing of human genome. Megabase-sized zones that replicate early or late in S phase (thus early/late transition) were defined at the sequence level. Using the newly replicated DNA from cell-cycle fractionated HCT116p53(+/+) and HCT1 116p53(-/-), the replication timing was determined and compared at the genome sequence level. In conclusion, we found that the early/late-switch regions of replication timing coincided with unstable regions of the genome and that genome-wide assessment of replication timing by DNA microarray serve as an efficient strategy for identifying cancer-related genes.
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