| Project/Area Number |
16510166
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Tokyo University of Pharmacy and Life Sciences |
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Principal Investigator |
IGUCHI Kazuo Tokyo University of Pharmacy and Life Sciences, School of Life Science, Professor, 生命科学部, 教授 (50057345)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | soft coral / Clavularia viridis / prostanoid / biosynthetic enzyme / lipoxygenase / 構造決定 / 20-ヒドロキシクラブロン |
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| Research Abstract |
The soft coral, Clavularia viridis, was collected for investigation of prostanoid components from the seawater off Ishigaki Islands (Okinawa Prefecture) in both October 2004 and May 2005. The coral samples were frozen by dry ices and transported to Tokyo. Living soft corals for the biosynthetic study were also collected and transported to Tokyo.
Wet soft corals were extracted with methanol. The ethyl acetate soluble portion of the methanol extract was subjected to chromatographic separation and purification to give three new marine prostanoids. The structures of these prostanoids including absolute configurations were elucidated on the basis of spectroscopic analysis.
Studies on isolation of biosynthetic enzyme for marine prostanoids in Clavularia viridis were also carried out. First the method for measuring the enzyme activity was established using UV absorptions. The 8-lipoxygenase (LOX) catalyzing the first step of biosynthesis was separated and purified from the homogenate of polyps of the soft coral using successively DEAE cellulose column, hydrophobic interaction column, and ion-exchange column, to give an active fraction. The specific activity of the active fraction was 900 times stronger than that of the homogenate.
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