Dynamics of dissolved organic nitrogen in the ocean obtained by using high-sensitive and high-resolution method

• Project/Area Number: 16540445
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Geochemistry/Astrochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: OGAWA Hiroshi The University of Tokyo, Ocean Research Institute, Associate Professor, 海洋研究所, 助教授 (50260518)
• Project Period (FY): 2004 – 2005
• Project Status: Completed (Fiscal Year 2005)
• Budget Amount: ¥3,500,000 (Direct Cost: ¥3,500,000); Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000); Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: Dissolved organic nitrogen(DON) / Dissolved organic carbon(DOC) / C : N ratio / High-sensitive and high-resolution analysis / Nitrogen cycling / Ocean / Elemental analysis / C:N比

Research Abstract

A high-sensitive and high-resolution method for measuring dissolved organic nitrogen (DON) in seawater has been established, which consists of a high-temperature combustion unit and a chemiluminescence detector. The international program for instrument comparison for measurement of DON in seawater confirmed that this system has a high performance to obtain accurate and precious data of DON in seawater. By using this system, the detailed distribution of DON in a variety of sea area and biological processes of DON in incubation experiments were obtained as follows: surface oceans in the western North Pacific along 165 E longitude and along A line of Oyashio region, Arakawa estuary in Tokyo Bay, and microbial decomposition process of DON in surface waters in the western. North Pacific and release process of DON by coral. The system is designed for simultaneous measurement of dissolved organic carbon (DOC) with DON, so that it has been made possible that DON dynamics is directly compared with those of DOC, and characteristics of C:N ratio of dissolved organic matter (DOM) is discussed. Consequently it was found that relatively C-rich DOM was stably accumulating in oligotrophic area and unproductive season, whereas N-rich DOM was temporally accumulating in high latitude area especially in productive season. Amino acids in DOM (Total Dissolved Amino acids: TDAA), as typical nitrogenous organic compounds and most major identifiable component of DON, were also determined for some oceanic samples. The proportions of amino acids' carbon (TDAA-C) to DOC (0.8-1.8%) and amino acids' nitrogen (TDAA-N) to DON (8-18%) were obtained. Both proportions of TDAA-C/DOC and TDAA-N/DON showed a tendency to be high in productive area of high latitude and low in oligotrophic area of low latitude, suggesting DOM in productive area contained more biogenic and fresh components, whereas more altered components mainly composed DOM in oligotrophic area.

Research Products

• [Journal Article] Effect of nitrate enrichment on release of organi c carbon and nitrogen from zooxanthellate coral, Acropora pulchra and Porites cylindrical (2006)
  • Author(s): Tanaka Y., T.Miyajima, Y.Umezawa, H.Fukuda, I.Koike, H.Ogawa, T.Hayashibara
  • Journal Title: Proceedings of the 10th International Coral Reef Symposium Pages: 925-931
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Temporal variability of stable carbon and nitrogen isotopic composition of size-fractionated particulate organic matter in the hypertrophic Sumida River Estuary of Tokyo Bay, Japan (2006)
  • Author(s): Sato T., T.Miyajima, H.Ogawa, Y.Umezawa, I.Koike
  • Journal Title: Estuarine, Coastal and Shelf Science 68 Pages: 245-258
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Translocation and conservation of organic nitrogen within the coral-zooxanthella symbiotic system of Acropora pulchra, as demonstrated by dual isotope-labeling techniques (2006)
  • Author(s): Tanaka Y, T.Miyajima, I.Koike, T.Hayashibara, H.Ogawa
  • Journal Title: J. Exp. Mar. Biol. Ecol. 印刷中
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Contribution of Water Soluble Organic Nitrogen to Total Nitrogen in Marine Aerosols over the East China Sea and Western North Pacific (2006)
  • Author(s): Nakamura T., H.Ogawa, D.K.Maripi, M.Uematsu
  • Journal Title: Atmospheric Environment 印刷中
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Effect of nitrate enrichment on release of organic carbon and nitrogen from zooxanthellate coral, Acropora pulchra and Porites cylindrical (2006)
  • Author(s): Tanaka Y., T.Miyajima, Y.Umezawa, H.Fukuda, I.Koike, H Ogawa, T.Hayashibara
  • Journal Title: Proceedings of the 10th International Coral Reef Symposium Pages: 925-931
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A direct instrument comparison for measurement of total dissolved nitrogen in seawater (2004)
  • Author(s): Sharp J.H.et al.(他14名 8番目)
  • Journal Title: Marine Chemistry 84 Pages: 181-193
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The decline and fate of an iron-induced subarctic phytoplankton bloom (2004)
  • Author(s): Boyd P.W.et al.(他37名 26番目)
  • Journal Title: Nature 428 Pages: 549-553
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A direct instrument comparison for measurement of total dissolved nitrogen in seawater (2004)
  • Author(s): Sharp, J.H., A.Y.Beauregard, D.Burdige, G.Cauwet, S.Curless, R.Lauck, K.Nagel, H.Ogawa, A.E.Parker, O.Primm, M.Pujo-pay, W.S.Savidge, S.Seitzinger, G.Spyres, R.Styles
  • Journal Title: Marine Chemistry 84 Pages: 181-193
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The decline and fate of an iron-induced subarctic phytoplankton bloom (2004)
  • Author(s): Boyd, P.W., C.S.Benner, C.S.Wong, Y.Nojiri, A.Tsuda, M.Levasseur, S.Takeda, R.Rivkin, P J.Harrison, R.Strzepek, J.Gower, R.M.Mckay, E.Abraham, M.Arychuk, J Barwell-clarke, W.Crawford, M.Hale, K.Harada, K.Johnson, H.Kiyosawa, I.Kudo, A.Marchetti, W.Miller, J.Needoba, J.Nishioka, H.Ogawa, J.Page, M.Robert, H.Saito, A.Sastri, N.Sherry, T.Soutar, N.Sutherland, Y.Taira, F.Whitney, Shau-King.E.Wong, T.Yoshimura
  • Journal Title: Nature 428 Pages: 549-553
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Determination of organic matter in seawater. (2004)
  • Author(s): H.Ogawa
  • Journal Title: In : Advanced analytical methods-from science ＆ technology to nano-＆ bio-technology-(in Japanese), NT.S, Tokyo Pages: 1000-1000
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Translocation and conservation of organic nitrogen within the coral-zooxanthella symbiotic system of Acropora pulchra, as demonstrated by dual isotope-labeling techniques
  • Author(s): Tanaka Y, T.Miyajima, I.Koike, T.Hayashibara, H.Ogawa
  • Journal Title: J Exp. Mar. Biol. Ecol. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Contribution of water soluble organic nitrogen to total nitrogen in marine aerosols over the East China Sea and Western North Pacific
  • Author(s): Nakamura T., H.Ogawa, D.K.Maripi, M.Uematsu
  • Journal Title: Atmospheric Environment (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 海水中の有機物定量、先端の分析法-理工学からナノ・バイオまで- (2004)
  • Author(s): 小川 浩史(分担)
  • Total Pages: 1000
  • Publisher: (株)エヌ・ティー・エス
  • Description: 「研究成果報告書概要(和文)」より