| Project/Area Number |
16550077
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Ehime University |
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Principal Investigator |
MANABE Takashi Ehime University, Faculty of Science, professor, 理学部, 教授 (60094281)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMAZAKI Yoji Ehime University, Faculty of Science, instructor, 理学部, 助手 (80284389)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2004: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | human plasma / plasma proteins / non-denaturing / two-dimensional gel electrophoresis / micro gel / mass spectrometry / MALDI-TOF MS / protein interaction / マイクロ / ESI-MS / MS / 生理的条件 |
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| Research Abstract |
Protein-protein interactions between human plasma proteins have been detected by micro two-dimensional electrophoresis (2-DE), comparing the patterns obtained under non-denaturing conditions and those obtained under partially denaturing conditions. The protein spots on the 2-DE gels, 157 spots on a non-denaturing micro 2-DE gel and 175 spots on a partially denaturing micro 2-DE gel, were subjected to structural analysis by mass spectrometry. For mass spectrometric analysis, matrix assisted laser desorption-time of flight mass spectrometry (MALDI-TOF MS) and database search (peptide mass fingerprinting, PMF) were employed. Integrating the information obtained, it was ascertained that structural information on human plasma proteins, including their status of complex-formation, can be analyzed. Although for the moment most of the protein researchers are using 2-DE under denaturing conditions, present study revealed that 2-DE under non-denaturing conditions will become more important by the advance of mass spectrometric methods in protein analysis.
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