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Fabrication of Molecular Recognition Sensor based on Molecular Imprinting-Chemiluminescence Detection

Research Project

Project/Area Number 16550081
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionTokyo Metropolitan University

Principal Investigator

YAMADA Masaaki  TMU, Graduate School of Urban Environmental Sciences, Dept. of Material Chemistry, Professor, 都市環境科学研究科, 教授 (10087322)

Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2006: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥3,000,000 (Direct Cost: ¥3,000,000)
KeywordsChemiluminescence / Molecular recognition / Molecular imprinting / Sensor element / Endocrine disrupting chemicals / Environmental analysis / Hazardous chemicals / Optical isomers / センサー素
Research Abstract

The diethylstilbestrol(DES) and luminol CL systems were applied to the determination of the endocrine disrupting chemicals (EDCs) such as estrogens and phthalate esters(PEs) using DES and luminol, respectively, as probe molecules. The EDCs are suspected to affect the endocrine system by the binding to estrogen receptor. Instead of the receptor, we employed the synthetic receptor obtained by molecular imprinting, which was a technique to prepare the molecularly imprinted polymer(MIP).
The estrogens such as 17 β-estradiol, estron and estriol were determined by the ability of the estrogens to displace DES from the MIP. The conversion of DES to a fluorescent compound by UV irradiation and then the employment of another CL system in place of the DES CL system made more sensitive determination of DES at 10^9〜10^6 M levels. Thus 17 β-estradiol as low as 10^7M was recognized and determined by the MIP. The method is expected to determine estrogens in river water.
The extent of the recognition of PEs by the MIP was dependent strongly on the molecular structure of the PEs. For this reason, the PEs were hydrolyzed to phthalic acid, which was determined by the ability of the phthalic acid to displace luminol from MIP. Thus total PEs as low as 10^8M levels were determined with no dependence of the kinds of PEs. The method was successfully applied to the determination of diethylphthalate at 10^7M levels in river water.
Moreover, the CL detection system for L-tryptophan was developed using a three-dimensional complex (4-vinylpyridine/Cu/L-Trp) as a probe molecule by the same method. L-Trp was recognized by the specific binding to the recognition site X(4-vinylpyridine/Cu/X) in the corresponding MIP and then determined by the luminous oxidation with hydrogen peroxide. The recognition between the D- and L-Trp was improved by the use of 1-vinylimidazol in place of 4-vinylpyridine as a functional monomer.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (13 results)

All 2007 2006 2005 2004

All Journal Article (13 results)

  • [Journal Article] 塩化オクチルトリメチルアンモニウム-ドデカン酸ナトリウム混合ベシクル溶液中でのルシゲニン化学発光を利用するスーパーオキシドの特異的検出2007

    • Author(s)
      針生泰史, 木村直也, 大野賢一, 山田正昭
    • Journal Title

      分析化学 (印刷中)

    • NAID

      110006291360

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Specific Detection of Superoxide Ion Using Lucigenin Chemiluminescence in Mixed Vesicles Formed from Aqueous Mixtures of Octyltrimethylammonium Chloride and Sodium Dodecanoate2007

    • Author(s)
      Yasushi Haryu
    • Journal Title

      Bunseki Kagaku (in print)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 塩化オクチルトリメチルアンモニウム-ドデカン酸ナトリウム配合ベシクル溶液中でのルシゲニン化学発光を利用するスーパーオキシドの特異的検出2007

    • Author(s)
      針生泰史, 木村直也, 大野賢一, 山田正昭
    • Journal Title

      分析化学 印刷中

    • Related Report
      2006 Annual Research Report
  • [Journal Article] フタル酸インプリントポリマーを用いた全フタル酸エステルのルミノール化学発光計測2006

    • Author(s)
      内川圭太, 大野賢一, 山田正昭
    • Journal Title

      日本分析化学会第55年会講演要旨集

      Pages: 267-267

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Determination of Total Phthalate Esters Using Phthalic Acid-Imprinted Polymers and Luminol Chemiluminescence2006

    • Author(s)
      Keita Uchikawa
    • Journal Title

      Proceeding of the 55th Annual Meeting of Japan Society of Analytical Chemistry

      Pages: 267-267

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 分子鋳型を用いるジエチルスチルベストロール認識ポリマーの作製とフェノール類の化学発光計測への応用2005

    • Author(s)
      春日一孝, 大野賢一, 山田正昭
    • Journal Title

      第66回分析化学討論会(日本分析化学会)講演要旨集

      Pages: 215-215

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 分子鋳型を用いたルミノール認識ポリマーの作製とフタル酸エステル類の化学発光計測への応用2005

    • Author(s)
      内川圭太, 大野賢一, 山田正昭
    • Journal Title

      日本分析化学会第54年会講演要旨集

      Pages: 256-256

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Preparation of Molecular Imprinting Polymer for Recognizing Diethylstilbestrol and Its Application to Chemiluminescent Determination of Phenols2005

    • Author(s)
      Kazutaka Kasuga
    • Journal Title

      Proceeding of the 66th Symposium of Japan Society of Analytical Chemistry

      Pages: 215-215

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Preparation of Molecular Imprinting Polymer for Recognizing Luminol and Its Application to Chemiluminescent Determination of Phthalate Esters2005

    • Author(s)
      Keita Uchikawa
    • Journal Title

      Proceeding of the 54th Annual Meeting of Japan Society of Analytical Chemistry

      Pages: 256-256

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 分子鋳型を用いるジエチルスチルベストロール認識ホリマーの作製とフェノール類の化学発光計測への応用2005

    • Author(s)
      春日一孝, 大野賢一, 山田正昭
    • Journal Title

      第66回分析化学討論会(日本分析化学会)講演要旨集

      Pages: 215-215

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 分子鋳型を用いたルミノール認識ポリマーの作製とフタル酸エステル類の化学発光計測への応用2005

    • Author(s)
      内川圭太, 大野賢一, 山田正昭
    • Journal Title

      日本分析化学会第54年会演要旨集

      Pages: 256-256

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 合成ホルモンdiethylstilbestrolの化学発光計測2004

    • Author(s)
      春日一孝, 穂谷野聡, 大野賢一, 山田正昭
    • Journal Title

      日本分析化学会第53年会講演要旨集

      Pages: 317-317

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Chemiluminescent Analysis of Artificial Hormone, Diethylstilbestrol2004

    • Author(s)
      Kazutaka, Kasuga
    • Journal Title

      Proceeding of the 53rd Annual Meeting of Japan Society of Analytical Chemistry

      Pages: 317-317

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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