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Kinetic study on enzymatic degradation of biodegradable polyesters

Research Project

Project/Area Number 16550139
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental chemistry
Research InstitutionRIKEN

Principal Investigator

YAMASHITA Koichi  RIKEN, Chemical Analysis Team, Senior Research Scientist, 化学分析チーム, 先任研究員 (90174672)

Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsbiodegradable polyester / enzymatic degradation / adsorption / QCM / esterase / AFM
Research Abstract

Enzymatic degradation of biopolyesters was investigated by a quartz crystal microbalance (QCM) and an atomic force microscope (AFM). In the case of enzymatic degradation of a melt-crystallized film of poly[(R)-3-hydroxybutyrate] (P(3HB)) by PHB depolymerase from Ralstonia pickettii T1, the erosion rate was not constant during the degradation due to heterogeneity of the film composed of an amorphous region and a crystalline region. Characteristic profiles were also observed for dependence of the erosion rate on depolymerase concentrations. The rate increased with enzyme concentrations to reach a maximum value at 1.0 μg/mL, and then decreased at higher enzyme concentrations. This dependence can be explained in terms of mutual steric hindrance among adsorbed enzyme molecules. The characteristic dynamic adsorption behavior of PHB depolymerase was also revealed. In contrast to the melt-crystallized P(3HB) film, the erosion rate of an amorphous poly [L-lactide] (PLLA) film by proteinase K was constant during degradation, indicating that the PLLA film is homogeneously amorphous. The erosion rate increased with enzyme concentration and remained constant under the conditions of [proteinase K] > 100 μg/mL. The erosion rate was proportional to the adsorbed amount of the enzymes. Adsorption of proteinase K was irreversible despite lack of the substrate-binding domain, so that the enzyme molecules on the film could be directly observed by AFM. Transformation of the molecules caused by packing in high density on the surface was observed at higher enzyme concentrations. The "footprint" of the individual proteinase K molecule on the PLLA film after enzymatic degradation suggests that the enzyme moves on the surface to hydrolyze the film around its. The differences between proteinase K and the PHB depolymerase might result from whether these enzymes possess the substrate-binding domain or not.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (13 results)

All 2007 2006 2005 Other

All Journal Article (13 results)

  • [Journal Article] Autoregulator Protein PhaR for Biosynthesis of Polyhydroxybutyrate [P(3HB)] Has Possible Two Separate Domains That Binds to the Target DNA and P(3HB) : Functional Mapping of Amino Acid Residues Responsible for DNA Binding2007

    • Author(s)
      Miwa Yamada
    • Journal Title

      J. Bacteriology 189・3

      Pages: 1118-1127

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Autoregulator Protein PhaR for Biosynthesis of Polyhydroxybutyrate [P(3HB)] Has Possible Two Separate Domains That Binds to the Target DNA and P(3HB) :2007

    • Author(s)
      Miwa Yamada
    • Journal Title

      J. Bacteriology

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Surface Properties and Enzymatic Degradation of End-capped Poly(1-lactide)2006

    • Author(s)
      Kenji Kurokawa
    • Journal Title

      Polymer Degradation and Stability 91・6

      Pages: 1300-1310

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nonspecific Hydrophobic Interactions of a Repressor Protein, PhaR, with Poly[(R)-3-hydroxybutyrate] Film Studied with a Quartz Crystal Microbalance2006

    • Author(s)
      Koichi Yamashita
    • Journal Title

      Biomacromolecules 7・8

      Pages: 2449-2454

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Surface Properties and Enzymatic Degradation of End-capped Poly(1-lactide)2006

    • Author(s)
      Kenji Kurokawa
    • Journal Title

      Polymer Degradation and Stability

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nonspecific Hydrophobic Interactions of a Repressor Protein, PhaR, with Poly[(R)-3-hydroxybutyrate] Film Studied with a Quartz Crystal Microbalance2006

    • Author(s)
      Koichi Yamashita
    • Journal Title

      Biomacromolecules

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Surface properties and enzymatic degradation of end-capped poly(l-lactide)2006

    • Author(s)
      Kenji Kurokawa
    • Journal Title

      Polym. Degrad. Stab. 91・6

      Pages: 1300-1310

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Enzymatic Degradation of Poly(L-lactide) Film by Proteinase K : Quartz Crystal Microbalance and Atomic Force Microscopy Study2005

    • Author(s)
      Koichi Yamashita
    • Journal Title

      Biomacromolecules 6・2

      Pages: 850-857

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Dynamic Adsorption Behavior of Poly(3-hydroxybutyrate) Depolymerase onto Polyester Surface Investigated by QCM and AFM2005

    • Author(s)
      Yoshihiro Kikkawa
    • Journal Title

      Biomacromolecules 6・4

      Pages: 2084-2090

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Enzymatic Degradation of Poly (L-lactide) Film by Proteinase K : Quartz Crystal Microbalance and Atomic Force Microscopy Study2005

    • Author(s)
      Koichi Yamashita
    • Journal Title

      Biomacromolecules

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Dynamic Adsorption Behavior of Poly(3-hydroxybutyrate) Depolymerase onto Polyester Surface Investigated by QCM and AFM2005

    • Author(s)
      Yoshihiro Kikkawa
    • Journal Title

      Biomacromolecules

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Enzymatic Degradation of Poly(L-lactide) Film by Proteinase K : Quartz Crystal Microbalance and Atomic Force Microscopy Study2005

    • Author(s)
      Koichi Yamashita
    • Journal Title

      Biomacromolecules 6・2(印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Functional Mapping of Amino Acid Residues Responsible for DNA Binding

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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