| Project/Area Number |
16550144
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Saga University |
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Principal Investigator |
KODAMA Hiroaki Saga University, Chemistry, Associate Professor, 理工学部, 助教授 (80205418)
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| Co-Investigator(Kenkyū-buntansha) |
OSADA Satoshi Saga University, Chemistry, Assistant Professor, 理工学部, 助手 (50284609)
小野 慎 富山大学, 工学部, 助教授 (10214181)
東元 祐一郎 久留米大学, 医学部, 助手 (40352124)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | transmembrane peptide / channel peptide / receptor protein / GPCR / neutrophil / superoxide production / priming / Intracelluler alcium ion / イオンチャンネル / ペプチド合成 / Aib / 分子架橋 / 二量体ペプチド |
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| Research Abstract |
Human formyl peptide receptor (FPR) mediates a number of important host defense functions. Although studies have been made on ligand binding site of FPR, FPR dynamic behaviors on cell surface is unknown. Recently, peptides derived from transmembrane (TM) domain of GPCRs were shown to disrupt dimer formation of receptors and results in specific regulation of receptor function. To reveal the function of FPR TM domains, hFPRTM peptides derived from human FPR were synthesized, and their biological activities were evaluated on human neutrophils. TM peptides were synthesized through a stepwise solid phase method using Fmoc amino acid on low substituted resin. The purifications of crude TM peptides were carried out by preparative RP-HPLC using water/2-propanol/formic acid system. The homogeneities and structures of synthetic peptides were verified by analytical RP-HPLC and MALDI-TOF MS. Synthetic hFPRTM peptides did not exhibit agonistic or antagonistic activities on superoxide anion production in human neutrophils. However, human neutrophils treated with hFPRTM4 produced 4-folds superoxide anion compared with untreated cells. Short peptides fragments from fourth TM region of human FPR did not enhance superoxide anion production, which suggest that hFPRTM4 did not behave as a ligand. CD and fluorescence spectra suggested that hFPRTM peptides inserted into the membrane and interacted with TM domain of membrane proteins. Addition of hFPRTM4 increased intracellular calcium concentration, which meant the peptide activate some membrane protein on cell surface. Present study suggests that the fourth TM domain of FPR has novel function related to priming effect.
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