| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Research Abstract |
Synthesis of dendrimer-type AIDS (acquired immunodeficiency syndrome) vaccine and its model was studied. Hemispherical and spherical polylysine dendrimers were prepared starting from (β-alanine and 1,4-butanediamine as the core, respectively. To the terminal 16 amino groups of spherical polylysine dendrimer G3,β-alanine, maltose, and succinic acid were successively added to produce succinyl-maltose-β-alanine-polylysine dendrimer G3. The carboxyl group of the dendrimer was reacted with a cyclic peptide sequence consisting of 27 amino acids of HIV gp 120. However, the dendrimer degradated during the condensation reaction, failing to give a vaccine. Similarly, succinyl-maltose-proline-polylysine dendrimer G3 also failed to form the vaccine carrying the cyclic peptide, though the vaccine model was obtained. A cellulose-polylysine dendrimer G3 having the reducing sugar terminals was synthesized via 9 steps reaction. The molecular weight measured by MALDI-TOF-MS was 4190, indicating that 8 cellobiose residues were bound at the terminal to form the dendrimer. To the cellobiose-polylysine dendrimer, were reacted a tripeptide and the cyclic peptide sequence by reductive amination. Although two tripeptides were connected per the dendrimer to produce a vaccine model, the reaction product with the cyclic peptide became insoluble. In addition, a lactose-polylysine dendrimer G3 was synthesized and reacted with a tripeptide succinyl-Ala-Pro-Ala-pNA to afford another vaccine model. In this study, three new reaction scheme were discovered to form the vaccine and its model. Finally, utilizing a recombinant yeast expressing β-glucosidases, a new process producing fuel ethanol from cellulosic materials like wood powder was discovered.
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