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Study for the detection of molecular target protein in cancer tissues by Dynamic Spot Method

Research Project

Project/Area Number 16560683
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionKyoto Institute of Technology (2005)
Osaka University (2004)

Principal Investigator

KISHIMOTO Michimasa  Kyoto Institute of Technology, Faculty of Engineering and Design, Professor, 工芸学部, 教授 (00144436)

Co-Investigator(Kenkyū-buntansha) SATO Seiji  Kyoto University, Graduate School of Medicine, Lecturer, 医学研究科, 講師 (00303834)
OHMASA Kenji  Osaka University, Graduate School of Engineering, Associate Professor, 工学研究科, 助教授 (00252586)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywords2-DE / Proteome analysis / Image analysis / Protein spot
Research Abstract

We developed an image analysis system for the detection of specific cancer protein spots in 2-DE gels. The system can analyze the time courses of silver stained gel images during developing processes, and distinguish the specific protein spots of cancer tissues from ones of non-cancer tissues.
We also developed an camera system which include the shaking system for performing the developing operation and stop the shaking intermittently for taking picture of the gel images. The system has an advantage of the free hand operation for taking pictures, and we need not transfer the gel from developing instrument to a camera system.
The combination of the image analysis and camera system can separate the overlapping spots, because we can get the information of original separated spots from the former images, and we can detect very faint spots because the system provide the later phase images which show the spots of only a few amount of protein relatively clear.
These results were presented in 2004 Annual Meeting of the American Electrophoresis Society.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (8 results)

All 2006 2005

All Journal Article (8 results)

  • [Journal Article] Recognition of Culture State Using Two-dimensional Gel Electrophoresis with an Artificial Neural Network2006

    • Author(s)
      Izawa N., Kishimoto M.他4名
    • Journal Title

      Proteomics (in print)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Recognition of Culture State Using Two-dimensional Gel Electrophoresis with an Artificial Neural Network2006

    • Author(s)
      Izawa, N., Kshimoto, M., et al.
    • Journal Title

      Proteomics (in printing)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Construction and evaluation of drug metabolizing bioartificial liver support system2005

    • Author(s)
      Omasa T., Kim K, 他5名
    • Journal Title

      Biotechnology Progress Vol.21

      Pages: 161-167

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effect of Sulfur Sources on Specific Desulfurization Activity of Rhodococcus erythropolis KA2-5-1 in Exponential Fed-Batch Culture2005

    • Author(s)
      Konishi M., Kishimoto, M.他3名
    • Journal Title

      Journal of Bioscience and Bioengineering Vol.99

      Pages: 258-263

    • NAID

      110002695656

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The separation of oil from an oil-water-bacteria mixture using a hydrophobic tubular membrane2005

    • Author(s)
      Konishi M., Kishimoto, M.他3名
    • Journal Title

      Biochemical Engineering Journal Vol.24

      Pages: 49-54

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Construction and evaluation of drug metabolizing bioartificial liver support system2005

    • Author(s)
      Omasa, T., Kim, K., et al.
    • Journal Title

      Biotechnology Progress 21

      Pages: 161-167

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effect of Sulfur Sources on Specific Desulfurization Activity of Rhodococcus erythropolis KA2-5-1 in Exponential Fed-Batch Culture2005

    • Author(s)
      Konishi, M., Kishimoto, M., et al.
    • Journal Title

      Journal of Bioscience and Bioengineering 99

      Pages: 258-263

    • NAID

      110002695656

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The separation of oil from an oil-water-bacteria mixture using a hydrophobic tubular membrane2005

    • Author(s)
      Konishi, M., Kishimoto, M., et al.
    • Journal Title

      Biochemical Engineering Journal 24

      Pages: 49-54

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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