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Research for the symbiotic system in a bioreactor where anaerobic ammonium oxidation proceeds.

Research Project

Project/Area Number 16560687
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionSojo University

Principal Investigator

FUJII Takao  Sojo Univeresity, Biotechnology and Life Science, Professor, 生物生命学部, 教授 (80165331)

Co-Investigator(Kenkyū-buntansha) FURUKAWA Kenji  Kumamoto University Graduate School, Science and Technology, Professor, 大学院自然科学研究科, 教授 (60029296)
杉野 浩幸  崇城大学, 工学部, 講師 (90258434)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsanaerobic ammonium oxidation / Anammox / denitrification / nitrite reductase / nir S / 嫌気性アンモニア酸化 / 脱窒 / 亜硫酸還元酵素 / nir S / 他栄養性脱窒菌 / 亜硝酸還元酵素
Research Abstract

Hydrazine-oxidizing enzyme (HZO) of an Anammox bacterium was characterized and the nucleotide sequence encoded by the HZO gene was determined. The enzyme has oxidizing activity towards hydrazine but not hydroxylamine. With cytochrome c as an electron acceptor, the Vmax and Km for hydrazine are 6.2±0.3 μmol/min・mg and 5.5±0.6 μM, respectively. Hydroxylamine work as a competitive inhibitor (Ki:2.5 μM) for hydrazine oxidation by HZO. The HZO appeared to be more than 10% of the total protein weight in the cells of the KSU-1 strain. It is likely that HZO play crucial roles in the catabolism of the anammox bacterium. Two genes, hzoA and hzoB encoded HZO. The expression of hzoB was 3 to 5 fold as much as that of hzoA.
Meta-genomic DNA was extracted from the sludge whose population was largely composed of an Anammox strain KSU-1, and analyzed. Besides the genes of strain KSU-1, the gene homologous to those of bacteria such as Geobacter, Roseiflexus, Anaeromyxobacter, Burkholderia, Kineococus. Azarcus, Syntrophus, Solibacter, Chloroflexus, Mthanosarcina, Blastopirellula, Leishmania, and Rhodopirellula were found. In the other hand, nitrite reductase genes (nir S) were amplified with nir S-specific primers and the DNA by PCR. However, the expression level of nir S was significantly low.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (14 results)

All 2007 2006 2005 2004

All Journal Article (14 results)

  • [Journal Article] Isolation of a Muttiheme Protein with Features of a Hydrazine-Oxidizing Enzyme from an Anaerobic Ammonium-Oxidizing Enrichment Culture2007

    • Author(s)
      M.Shimamura, T.Nishiyama, H.Shigetomo, T.Toyomoto, Y.Kawahara, K.Furukawa, T.Fujii
    • Journal Title

      Applied Environmental Microbiology 73

      Pages: 1065-1072

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Isolation of a Multiheme Protein with Features of a Hydrazine-Oxidizing Enzyme from an Anaerobic Ammonium-Oxidizing Enrichment Culture2007

    • Author(s)
      M.Shimamura, T.Nishiyama, H.Shigetomo, T.Toyomoto, Y.Kawahara, K.Furukawa, T.Fujii
    • Journal Title

      Applied Environmental Microbiology 73

      Pages: 1065-1072

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Isolation of a Multiheme Protein with Features of a Hydrazine-Oxidizing Enzyme from an Anaerobic Ammonium-Oxidizing Enrichment Culture.2007

    • Author(s)
      M.Shimamura, T.Nishiyama, H.Shigetomo, T.Toyomoto, Y.Kawahara, K.Furukawa, T.Fujii
    • Journal Title

      Applied Environmental Microbiology 73

      Pages: 1065-1072

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Characteristics of Sludge from Single-Stage Nitrogen Removal Using Anammox and Partial Nitritation (SNAP)2006

    • Author(s)
      P.K.Lieu, H.Homan, A.Kurogi, Y.Kawagoshi, T.Fujii, K.Furukawa
    • Journal Title

      Water Science Technology 53

      Pages: 83-90

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nitrogen removal by immobilized anammox sludge using PVA gel as bio-carrier2006

    • Author(s)
      T.H.Hoa, L.N.Khanh, Z.Liu, T.Fujii, J.D.Rouse, K.Furukawa
    • Journal Title

      Japanese Journal of Water Treatment Biology 42

      Pages: 139-149

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Characteristics of Sludge from Single-Stage Nitrogen Removal Using Anammox and Partial Nitriation(SNAP).2006

    • Author(s)
      P.K.Lieu, H.Homan, A.Kurogi, Y.Kawagoshi, T.Fujii, K.Furukawa
    • Journal Title

      Japanese J.of Water Treatment Biology 42

      Pages: 53-64

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Nitrogen removal by immobilized anammox sludge PVA gel as biocarrere2006

    • Author(s)
      T.H.Hoa, L.N.Khanh, Z.Liu, T.Fujii, J.D.Rouse, K.Furukawa
    • Journal Title

      Japanese J.of Water Treatment Biology 42

      Pages: 139-149

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Evaluation of the swim-bed attached-growth process for nitrification of Hanoi Groundwater containing high levels of iron2005

    • Author(s)
      D.T.HA, R.KUTSUMOTO, T.KOYAMA, T.FUJII, K.FURUKAWA
    • Journal Title

      Japanese Journal of Water Treatment Biology 41

      Pages: 181-192

    • NAID

      10017093469

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Evaluation of the swim-bed attached-growth process for nitrification of Hanoi Groundwater containing high levels of iron2005

    • Author(s)
      D.T.HA, R.KUTSUMOTO, T.KOYAMA, T.FUJII, K.FURUKAWA
    • Journal Title

      Jap.J.Water Treat.Biol. 41

      Pages: 181-192

    • NAID

      10017093469

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Single-stage nitrogen removal using Anammox and partial nitrification2004

    • Author(s)
      K.Furukawa, H.Tokitoh, Pham K.Lieu, T.Fujii
    • Journal Title

      Proceedings of Shino-Japanese forum on protection and restoration of water environment (CD-ROM)

      Pages: 179-186

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 活性汚泥からのAnammox微生物の集積培養2004

    • Author(s)
      今城麗, 安井英斉, 石田浩昭, 藤井隆夫, 杉野浩幸, 古川憲治
    • Journal Title

      水環境学会誌 27

      Pages: 413-418

    • NAID

      10013080142

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Treatment potential for the Anammox process2004

    • Author(s)
      K.Furukawa, J.D.Rouse, T.Takagi, T.Fujii, H.Sugino
    • Journal Title

      Proceeding of 2004 ASA-CCSA-SSCA international annual meetings (CD-ROM)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Single-stage nitrogen removal using Anammox and partial nitrifycation2004

    • Author(s)
      K.Furukawa, H.Tokitoh, Pham K.Lieu, T.Fujii
    • Journal Title

      Proceedings of Shino-Japanese forum on protection and restoration of water environment

      Pages: 179-186

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 活性汚泥からのAnammox微生物の集積培養2004

    • Author(s)
      今城麗, 安井英斉, 石田浩昭, 藤井隆夫, 杉野浩幸, 古川健司
    • Journal Title

      水環境学会誌 27

      Pages: 413-418

    • NAID

      10013080142

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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