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Design of nano-particle type drug delivery system with thermosensitive bioelastic polypeptides by radiation crosslinking

Research Project

Project/Area Number 16560736
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Nuclear engineering
Research InstitutionOsaka Prefecture University

Principal Investigator

FURUTA Masakazu  Osaka Prefecture University, Graduate School of Sciences, Associate Professor, 理学系研究科, 助教授 (40181458)

Co-Investigator(Kenkyū-buntansha) OKUDA Shuichi  Osaka Prefecture University, Frontier Science Innovation Center, Professor, 産学官連携機構, 教授 (00142175)
HARA Masayuki  Osaka Prefecture University, Graduate School of Sciences, Professor, 理学系研究科, 教授 (50344172)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsnano material / thermosensitivity / polypeptide / LCST / radiation crosslinking / エラスチン / ナノ粒子 / DDS
Research Abstract

Elastic protein-based polymers were prepared by recombinant DNA technology and based on the GVGVP repeat amino acid sequence of tropoelastin. Various heating condition was examined for aqueous solutions of the polypeptides to obtain an appropriate nano-particle suspensions. Under the specified condition of "heat shock" ; dropping the concentrated solution into the hot water (40℃), they can be □-irradiation cross-linked to form nano-particles that pass through a 0.22nm filter and exhibit diameters of less than ca.200nm and a narrow size distribution. Yield of the □-cross-linked nano-particles increases with increasing dose above 8.5 kGy but is insensitive to dose rate. We confirmed the particles by TEM. GPC and SDS-PAGE analyses strongly suggested that gamma-rays caused mostly crosslinking within the particles. Time-release profiles for Biebrich Scarlet Red indicated continuous release of the dye for more than 2 weeks. Thus, this controlled release device can deliver drugs to sites accessible by small caliber hypodermic syringes.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (12 results)

All 2005 2004

All Journal Article (9 results) Book (3 results)

  • [Journal Article] Gelatin hydrogels cross-linked by gamma-ray irradiation : materials for absorption and release of dye2005

    • Author(s)
      M.Bessho
    • Journal Title

      Journal of Biomater.Sci.Polymer Edn. 16(6)

      Pages: 715-724

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Gelatin hydrogels cross-linked by gamma-ray irradiation : materials for absorption and release of dye,2005

    • Author(s)
      M.Bessho, M.Furuta, T.Kojima, S.Okuda, M.Hara
    • Journal Title

      Journal of Biomater.Sci.Polymer Edn. 16(6)

      Pages: 715-724

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Synthesis and Biomaterials Application of Polymers Containing Pentapeptide and/or Hexapeptide Sequences Derived from Elastin2005

    • Author(s)
      Kouji Okamoto
    • Journal Title

      BIOPOLYMERS 80・4

      Pages: 582-582

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Pulse radiolysis system of OPU-LINAC in RIAST, Osaka Prefecture University2004

    • Author(s)
      T.Kojima
    • Journal Title

      Radiation Physics and Chemistry 71(1-2)

      Pages: 603-606

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of biopolymer hydrogels produced by γ-ray irradiation2004

    • Author(s)
      T.Kojima
    • Journal Title

      Radiation Physics and Chemistry 71(1-2)

      Pages: 235-238

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Protective effect of poly (α-L-glutamate) against UV and y-irradiation2004

    • Author(s)
      M.Furuta
    • Journal Title

      Radiation Physics and Chemistry 71

      Pages: 113-116

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Pulse radiolysis system of OPU-LINAC in RIAST, Osaka Prefecture University,2004

    • Author(s)
      T.Kojima, R.Taniguchi, M.Furuta, S.Okuda, M.Hara, S.Fujita
    • Journal Title

      Radiation Physics and Chemistry 71(1-2)

      Pages: 603-606

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of biopolymer hydrogels produced by γ-ray irradiation,2004

    • Author(s)
      T.Kojima, M.Bessho, M.Furuta, S.Okuda, M.Hara
    • Journal Title

      Radiation Physics and Chemistry 71(1-2)

      Pages: 235-238

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Protective effect of poly (α-L-glutamate) against UV and gamma-irradiation.2004

    • Author(s)
      M.Furuta, N.Q.Huy, A.Tsuchiya, H.Nakatsuka, T.Hayashi
    • Journal Title

      Radiation Physics and Chemistry 71

      Pages: 113-116

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Elastic Protein-Based Elements of Basic Sciences, Controled Release, and Biocmpatibility In "Tissue Engineering and Novel Delivery Systems"2004

    • Author(s)
      D.W.Urry
    • Total Pages
      620
    • Publisher
      Marcel Dekker, Inc. New York
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Elastic Protein-Based Elements of Basic Sciences, Controlled Release, and Biocompatibility, Tissue Engineering and Novel Delivery Systems(Marcel Dekker)2004

    • Author(s)
      D.W.Urry, T.C.Woods, L.C.Hayes, J.Xu, D.T.McPherson, T.M.Parker, M.Iwama, M.Furuta, T.Hayashi, M.Murata
    • Publisher
      Inc.New York
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] "Elastic Protein-Based Elements of Basic Sciences, Controled Release, and Biocmpatibility", In "Tissue Engineering and Novel Deliver Systems"2004

    • Author(s)
      D.W.Urry, M.Iwama, M.Furuta (他7名)
    • Total Pages
      260
    • Publisher
      Mercel Deckker, Inc.New York
    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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