Studies on an arbuscular mycorrhiza-inducible phosphate transporter gene of Lotus japonicus
| Project/Area Number |
16570032
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | Kyoto University |
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Principal Investigator |
HATA Shingo Kyoto University, Graduate School of Biostudies, Associate Professor, 生命科学研究科, 助教授 (40238001)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Arbuscular mycorrhizal fungi / Phosphate transporter / Symbiosis / Rhizobia / Defense response / Lotus japonicus / リン酸輸送体 / 窒素固定根粒 / アーバスキュラー菌根 / リン酸トランスポータ |
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| Research Abstract |
cDNA for a major arbuscular mycorrhiza (AM)-inducible phosphate (Pi) transporter of Lotus japonicus, LjPT3, was isolated from Glomus mosseae-colonized roots. The LjPT3 transcript was expressed in arbuscule-containing cells of the inner cortex. The transport activity of the gene product was confirmed by the complementation of a yeast mutant that lacks high-affinity Pi transporters. In contrast to most AM-inducible Pi transporters thus far reported, LjPT3 has an amino acid sequence that has much in common with those of other members of the Pht1 family of plant Pi transporters, like StPT3 of potato [Rausch et al. (2001) Nature 414:462-470]. To better understand the physiological role of this AM-inducible Pi transporter, knockdown transformants of the gene were prepared through hairy root transformation and RNA-interference. Under Pi-limiting conditions, the transformants showed a reduction of Pi uptake via AM and growth retardation. The transformants also exhibited a decrease in G. mosseae arbuscules. Additionally, when Mesorhizobium loti was inoculated into the knockdown transformants in combination with G. mosseae, necrotic root nodules were observed. Based on these findings, we consider that the genetically engineered host plants had monitored insufficient Pi uptake via AM or low expression of LjPT3, excluding the existing fungi and rhizobia and/or preventing further development of the fungal and nodule structures.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Genetics of symbiosis in Lotus japonicus : recombinant inbred lines, comparative genetic maps and map position of 35 symbiotic loci.2006
Author(s)
Sandal N, Petersen TR, Murray J, Umehara Y, Karas B, Yano K, Kumagai H, Yoshikawa M, Saito K, Hayashi M, Murakami Y, Wang X, Hakoyama T, Imaizumi-Anraku H, Sato S, Kato T, et al.
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Journal Title
Mol.Plant-Microbe Interact. 19
Pages: 80-91
Related Report
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[Journal Article] Characterization of the Lotus japonicus symbiotic mutant lot1 that shows a reduced nodule number and distorted trichomes.2005
Author(s)
Ooki Y, Banba M, Yano K, Maruya J, Sato S, Tabata S, Saeki K, Hayashi M, Kawaguchi M, Izui K, Hata S.
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Journal Title
Plant Physiol. 137
Pages: 1261-1271
Related Report
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[Journal Article] Characterization of a Lotus japonicus symbiotic mutant, lot1, that shows a reduced nodule number and distorted trichomes2005
Author(s)
Ooki Y, Banba M, Yano K, Maruya J, Sato S, Tabata S, Saeki K, Hayashi M, Kawaguchi M, Izui K, Hata S.
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Journal Title
Plant Physiol. (In press)
Related Report
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