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Studies on an arbuscular mycorrhiza-inducible phosphate transporter gene of Lotus japonicus

Research Project

Project/Area Number 16570032
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理・分子
Research InstitutionKyoto University

Principal Investigator

HATA Shingo  Kyoto University, Graduate School of Biostudies, Associate Professor, 生命科学研究科, 助教授 (40238001)

Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsArbuscular mycorrhizal fungi / Phosphate transporter / Symbiosis / Rhizobia / Defense response / Lotus japonicus / リン酸輸送体 / 窒素固定根粒 / アーバスキュラー菌根 / リン酸トランスポータ
Research Abstract

cDNA for a major arbuscular mycorrhiza (AM)-inducible phosphate (Pi) transporter of Lotus japonicus, LjPT3, was isolated from Glomus mosseae-colonized roots. The LjPT3 transcript was expressed in arbuscule-containing cells of the inner cortex. The transport activity of the gene product was confirmed by the complementation of a yeast mutant that lacks high-affinity Pi transporters. In contrast to most AM-inducible Pi transporters thus far reported, LjPT3 has an amino acid sequence that has much in common with those of other members of the Pht1 family of plant Pi transporters, like StPT3 of potato [Rausch et al. (2001) Nature 414:462-470]. To better understand the physiological role of this AM-inducible Pi transporter, knockdown transformants of the gene were prepared through hairy root transformation and RNA-interference. Under Pi-limiting conditions, the transformants showed a reduction of Pi uptake via AM and growth retardation. The transformants also exhibited a decrease in G. mosseae arbuscules. Additionally, when Mesorhizobium loti was inoculated into the knockdown transformants in combination with G. mosseae, necrotic root nodules were observed. Based on these findings, we consider that the genetically engineered host plants had monitored insufficient Pi uptake via AM or low expression of LjPT3, excluding the existing fungi and rhizobia and/or preventing further development of the fungal and nodule structures.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (16 results)

All 2007 2006 2005 2004

All Journal Article (16 results)

  • [Journal Article] Temporary expression of the TAF1O gene and its requirement for normal development of Arabidopsis thaliana.2007

    • Author(s)
      Tamada Y, Nakamori K, Nakatani H, Matsuda K, Hata S, Furumoto T, Izui K
    • Journal Title

      Plant Cell Physiol. 48(1)

      Pages: 134-146

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Knockdown of an arbuscular mycorrhiza-inducible phosphate transporter gene of Lotus japonicus suppresses mutualistic symbiosis.2006

    • Author(s)
      Maeda D, et al.
    • Journal Title

      Plant and Cell Physiology 47(7)

      Pages: 807-817

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Phosphoenolpyruvate carboxylase plays a crucial role for limiting nitrogen fixation in Lotus japonicus nodules.2006

    • Author(s)
      Nomura M, et al.
    • Journal Title

      Plant and Cell Physiology 47(5)

      Pages: 613-621

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genetics of symbiosis in Lotus japonicus : Recombinant inbred lines, comparative genetic maps and map position of 35 symbiotic loci.2006

    • Author(s)
      Sandal N, et al.
    • Journal Title

      Molecular Plant-Microbe Interactions 19(1)

      Pages: 80-91

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genetics of symbiosis in Lotus japonicus : Recombinant inbred lines, comparative genetic maps and map position of 35 symbiotic loci.2006

    • Author(s)
      Sandal N, et al.
    • Journal Title

      Molecular Plant Microbe Interactions 19(1)

      Pages: 80-91

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Phosphoenolpyruvate carboxylase plays a crucial role for limiting nitrogen fixation in Lotus japonicus nodules.2006

    • Author(s)
      Nomura M, Mai HT, Fujii M, Hata S, Izui K, Tajima S.
    • Journal Title

      Plant Cell Physiol. 47(5)

      Pages: 613-621

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Knockdown of an arbuscular mycorrhiza-inducible phosphate transporter gene of Lotus japonicus suppresses mutualistic symbiosis.2006

    • Author(s)
      Maeda D, Ashida K, Iguchi K, Chechetka SA, Deguchi Y, Izui K, Hata S.
    • Journal Title

      Plant Cell Physiol. 47(7)

      Pages: 807-817

    • NAID

      10018185102

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Genetics of symbiosis in Lotus japonicus : recombinant inbred lines, comparative genetic maps and map position of 35 symbiotic loci.2006

    • Author(s)
      Sandal N, Petersen TR, Murray J, Umehara Y, Karas B, Yano K, Kumagai H, Yoshikawa M, Saito K, Hayashi M, Murakami Y, Wang X, Hakoyama T, Imaizumi-Anraku H, Sato S, Kato T, et al.
    • Journal Title

      Mol.Plant-Microbe Interact. 19

      Pages: 80-91

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Characterization of the Lotus japonicus symbiotic mutant lot1 that shows a reduced nodule number and distorted trichomes.2005

    • Author(s)
      Ooki Y, et al.
    • Journal Title

      Plant Physiology 137(4)

      Pages: 1261-1271

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Vasucular-tissue abundant expression of plant TAF10, an orthologous gene for TATA box-binding protein-associated factor 10, in Flaveria trinervia and abnormal morphology of Arabidopsis thaliana transformants on its over-expression.2005

    • Author(s)
      Furumoto T, Tamada Y, Izumida A, Nakatani H, Hata S, Izui K.
    • Journal Title

      Plant Cell Physiol. 46

      Pages: 108-117

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Characterization of the Lotus japonicus symbiotic mutant lot1 that shows a reduced nodule number and distorted trichomes.2005

    • Author(s)
      Ooki Y, Banba M, Yano K, Maruya J, Sato S, Tabata S, Saeki K, Hayashi M, Kawaguchi M, Izui K, Hata S.
    • Journal Title

      Plant Physiol. 137

      Pages: 1261-1271

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Characterization of a Lotus japonicus symbiotic mutant, lot1, that shows a reduced nodule number and distorted trichomes2005

    • Author(s)
      Ooki Y, Banba M, Yano K, Maruya J, Sato S, Tabata S, Saeki K, Hayashi M, Kawaguchi M, Izui K, Hata S.
    • Journal Title

      Plant Physiol. (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] cDNA macroarray analysis of gene expression in ineffective nodules induced on the Lotus japonicus senl mutant.2004

    • Author(s)
      Suganuma N, et al.
    • Journal Title

      Molecular Plant-Microbe Interactions 17(11)

      Pages: 1223-1233

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Large-scale analysis of gene expression profiles during early stages of root nodlule formation in a model legume, Lotus japonicus.2004

    • Author(s)
      Kouchi H, et al.
    • Journal Title

      DNA Research 11(4)

      Pages: 263-274

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] cDNA macroarray analysis of gene expression in ineffective nodules induced on the Lotus japonicus sen1 mutant.2004

    • Author(s)
      Suganuma N, et al.
    • Journal Title

      Molecular Plant-Microbe Interactions 17(11)

      Pages: 1223-1233

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cloning and expression analysis of a MAPKKK gene and a novel nodulin gene of Lotus japonicus.2004

    • Author(s)
      Kinoshita N, Ooki Y, Deguchi Y, Chechetka SA, Kouchi H, Umehata Y, Izui K, Hata S.
    • Journal Title

      Biosci Biotechnol Biochem. 68(8)

      Pages: 1805-1807

    • NAID

      10013521198

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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