Elucidation of auxin signaling mechanism through identification of antiauxin resistant gene
| Project/Area Number |
16570042
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | Japan Atomic Energy Agency |
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Principal Investigator |
OONO Yutaka Japan Atomic Energy Agency, Quantum Beam Science Directrate, 量子ビーム応用研究部門, 研究副主幹 (30343940)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Arab idop sis / auxin / antiauxin / PCIB / 2,4-D / aarl / ion beam / SMAP / aar1 |
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| Research Abstract |
An antiauxin resistant1 (aar1) mutant has been identified in a screen for resistance to the antiauxin, p-chlorophenoxy-isobutyric acid (PCIB) The aar1 was also resistant to synthetic auxin 2,4-D. To understand molecular mechanism of auxin action, genetic analyses of aar1 mutant were carried out. The aarl-1 allele harbors multi-gene deletions in T6G15 region on chromosome 4. 2,4-D responsiveness was restored by transformation with one of the genes missing and the 2,4-D-resistant phenotype was reproduced by decreasing the expression of the same gene in wild type with a RNAi construct. The gene encodes a small, acidic protein (SMAP1) with unknown function and present in plants, animals, and invertebrates but not in fungi or prokaryotes. GFP fusion analysis of SMAP1 suggested that SMAP1 is localized in nucleus and its C-terminal region is functionally important. Taken together, the results implied the presence of a novel auxin-signaling pathway through conserved protein SMAP1.
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Report
(4 results)
Research Products
(3 results)
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[Journal Article] A small acidic protein 1 (SMAP1) mediates responses of the arabidopsis root to the synthetic auxin 2,4-dichlorophenoxyacetic acid2006
Author(s)
Rahman, A., Nakasone, A, Chhun, T., Ooura, C., Biswas, K.K., Uchimiya, H., Tsurumi, S., Baskin, T.I., Tanaka, A., Oono, Y.
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Journal Title
Plant Journal 47
Pages: 788-801
Description
「研究成果報告書概要(欧文)」より
Related Report
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