| Project/Area Number |
16570044
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
JIANG Change-Jie National Institute of Agrobiological Sciences, Plant Disease Research Unit, Division of Plant Science, Chief Researcher, 植物科学研究領域・耐病性研究ユニット, 主任研究員 (80370659)
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| Co-Investigator(Kenkyū-buntansha) |
TAKATSUJI Hiroshi National Institute of Agrobiological Sciences, Plant Disease Research Unit, Division of Plant Science, Head, 植物科学研究領域・耐病性研究ユニット, ユニット長 (10360455)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Lateral shoot branching / Transcriptional factor / Zinc finger / LIF (Lateral Inducing Factor) / Superman / SPL (Superman-like) / SPL(Superman-Like) |
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| Research Abstract |
Petunia Lateral-shoot Inducing Factor (LIF) is specifically expressed at the basal region of axillary buds and drastically increases lateral shoot branching when overexpressed in transgenic plants (LIF-OX). In this study, we investigated the molecular function of LIF in the control of lateral shoot branching with a focus on its relation to phytohormones. We also performed a comprehensive functional analysis of SUP/LIF family genes in Arabidopsis (Superman-Like, AtSPL) and found that AtSPL5, a new AtSPL gene, is implicated in responses to environmental stresses and phytohormones.
Determination of phytohormone contents indicated that the levels of cytokinin metabolites in LIF-OX petunia plants were significantly changed in comparison to wild type plants, with remarkable decrease in basal t-Zeatin level, which we presume to correlate with the promotion of lateral-shoot branching by LIF. Microarray analysis using LIF-inducible transgenic Arabidopsis plants in the GVG system revealed a number of LIF-regulated genes including those for transcription factors and tau-type GSTs.
The expression of AtSPLS in young seedlings was downregulated in response to environmental stresses, drought, salt, ozone and UV-B ; and phytohormones salicylic acid (SA) and abscisic acid (ABA). RNAi-mediated knockdown of AtSPL5 resulted in an increase in expression of ABA-responsive genes rd29A/B. Given that AtSPLS contain an EAR-motif-like transcriptional repression domain at its C-terminal, our results suggest that AtSPL5 is involved in suppressing the expression of stress responsive genes of plants during their growth under normal environmental conditions.
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