| Project/Area Number |
16570046
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | The Institute for Physical and Chemical Research (RIKEN) |
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Principal Investigator |
YAMAMOTO Yoshiharu RIKEN, Acceleration Development Group, Research Associate, 加速器利用展開グループ, 加速器研究員 (50301784)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | environmental response / photosynthesis / chloroplast / gene expression / light stress / Arabidopsis / microarray / luciferase / アイクロアレイ |
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| Research Abstract |
Response to high light, drought, and reactive oxygen was analyzed using microarray, and genes under specific regulation by high light were identified. I have prepared promoter database of Arabidopsis, and with the aid of it, I tried to identify cis regulatory elements for high light-specific gene activation. Firstly, I extracted overrepresented elements among the high light-activated promoter group compared with whole Arabidopsis promoters. Some of these cis candidates were found in the ELIP2 promoter, which gives strong high light response when fused to the luciferase reporter in transgenic Arabidopsis. Together with some other analyses, i could identify two putative high light responsive elements.
A line of transgenic Arabidopsis that shows transcriptional response to light stress as activation of luciferase activity was used for screening mutants with altered high light response. Several isolated mutants were subjected to physiological analyses in order to classification of the mutants. These analyses revealed that there are two types of mutants, mutants with the disturbed signal transduction and ones with disordered photosynthetic apparatus.
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