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Analysis of behavior of different histones after nuclear fusion.

Research Project

Project/Area Number 16570057
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionYokohama City University

Principal Investigator

TANAKA Ichiro  Yokohama City University, International Graduate School of Arts and Sciences, Professor, 国際総合科学研究科, 教授 (60175445)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsNucleus / Chromatin / Cell fusion / Interspecific hybrid / Chromosome / Gamete / Histone / Reporter gene
Research Abstract

It is unknown how the chromatin proteins including histones are distributed in fused nucleus between two kinds of different cells. In this study, in order to clarify the distribution of different histones, we tried to analyze the fused nucleus after in vitro cell fusion using GFP (green fluorescent protein) as a reporter gene. For this purpose, we first produced transgenic tobacco that was introduced cDNA of gH1(male gametic nucleus-highly concentrated histone H1 variant of Lilium longiflorum) with GFP by Agrobacterium-mediated system. Next, we fused protoplasts isolated from mesophyll cells of the transgenic tobacco with protoplasts isolated from tobacco cultured BY-2 cells by an electric pulse. Each protoplast can easily be distinguished by existence of chloroplasts. As a result, about 15% of cell fusion was induced between two kinds of protoplasts. Each fusion product was successively observed under fluorescent microscope after staining with DAPI. After 12 hr of cell fusion, fused nucleus, in which GFP signal was concentrated in one portion within DAPI signal, was observed. It is now be investigating whether the nucleus is directly fused or divided one. Thus, this study succeeded in observing nuclear fusion using labeled intact cells.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (8 results)

All 2006 2005 2004

All Journal Article (7 results) Book (1 results)

  • [Journal Article] Expression analysis of genes encoding plasma membrane aquaporins during seed and fruit development in tomato.2006

    • Author(s)
      Shiota, H. et al.
    • Journal Title

      Plant Science (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A histone H3.3-like gene specifically expressed in the vegetative cell of developing lily pollen.2005

    • Author(s)
      Sano, Y., Tanaka, I.
    • Journal Title

      Plant and Cell Physiology 46

      Pages: 1299-1308

    • NAID

      40006818161

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Male gametic cell-specific histone gH2A gene of Lilium Iongiflorum : genomic structure and promoter activity in the generative cell.2005

    • Author(s)
      Ueda, K. et al.
    • Journal Title

      Plant Molecular Biology 59

      Pages: 229-238

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Male gametic cell-specific histone gH2A gene of Lilium longiflorum : genomic structure and promoter activity in the generative cell.2005

    • Author(s)
      Ueda, K. et al.
    • Journal Title

      Plant Molecular Biology 59

      Pages: 229-238

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A histone H3.3-like gene specifically expressed in the vegetative cell of developing lily pollen.2005

    • Author(s)
      Y.Sano, I.Tanaka
    • Journal Title

      Plant and Cell Physiology 46

      Pages: 1299-1308

    • NAID

      40006818161

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Male gametic cell-specific histone gH2A gene of Lilium longiflorum : genomic structure and promoter activity in the generative cell.2005

    • Author(s)
      K.Ueda et al.
    • Journal Title

      Plant Molecular Biology 59

      Pages: 229-238

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Isolation and characterization of six abscisic acid-inducible genes from carrot somatic embryos.2004

    • Author(s)
      Shiota, H. et al.
    • Journal Title

      Plant Biotechnology 21

      Pages: 309-314

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] クロモソーム-植物染色体研究の方法-2006

    • Author(s)
      田中一朗, 上田健治
    • Total Pages
      274
    • Publisher
      養賢堂
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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