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Study of maturation-inducing steroid hormone receptor of fish

Research Project

Project/Area Number 16570059
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionNational Institute for Basic Biology

Principal Investigator

YOSHIKUNI Michiyasu  National Institute for Basic Biology, Lab.of Reproductive Biology, associate professor, 生殖生物学研究部門, 助教授 (50210662)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordsoocyte maturation / steroid hormone / membrane receptor / GPCR / progestin / procesterone / プロテオミクス / プロテオーム解析 / Proteomics
Research Abstract

The beta-type of progestin membrane receptor of medaka was newly cloned and registered as olGPCRβ with the accession number of AB239928. With this cloning we have collected all supposed three types of G-protein coupled membrane receptors for maturation-inducing steroid hormone of medaka (Oryzias latipes). Northern blot analyses showed that olGPCRα was expressed in brain, head kidney, muscle, ovary and testis, and olGPCRγ was expressed in ovary and testis. The expression of olGPCRβ was not detected in any tissues. We have never succeeded to generate recombinant receptor proteins although we had tried to express by using E.coli and HEK293 cell line.
In this project, we have been trying to generate specific polyclonal antibodies against synthetic peptides designed with predicted peptide sequences of these three receptor genes. All generated antibodies with acceptable titers against antigen peptides have been examined to detect receptor proteins by Western blotting analyses. However, we hav … More e never succeeded to get usable antibody for native receptor proteins.
We cloned medaka homologs (olPGRMC1 ; AB240046, olPGRMC2 ; AB23991) of human progesterone binding proteins, Hpr6.6 and Dg6. We have also found medaka sex hormone-binding protein from TIGR database (TC41349). One possible nuclear progestin receptor has been identified from medaka genome database (scaffold 5161) although it still needs detailed verification to distinguish from corticoid receptor.
At the end of this project we have shown successfully that medaka has 4groups/7 proteins as the receptor or the binding-protein of progestin as follows ;
1.G-protein coupled receptor : olGPCRa, olGPCRb, olGPCRg
2.nuclear receptor medaka PR
3.human membrane progesterone receptor homologs : olPGRMC1, olPGRMC2
4.Sex-hormone binding protein : medaka SHBG
We will analyze the expression levels and activities of all seven proteins along the development of medaka from fertilized egg to adult fish to find some new functions and correlations among these proteins. Less

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (14 results)

All 2006 2005 2004

All Journal Article (14 results)

  • [Journal Article] Phosphorylation of the p34cdc2 target site on goldfish germinal vesicle lamin B3 before oocyte maturation.2006

    • Author(s)
      Akihiko Yamagudhi
    • Journal Title

      European Journal of Cell Biology 85

      Pages: 501-517

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Phosphorylation of the p34cdc2 target site on goldfish germinal vesicle lamin B3 before oocyte maturation.2006

    • Author(s)
      Yamaguchi A., Katsu Y., Matsuyama M., Yoshikuni M., Nagahama Y
    • Journal Title

      European Journal of Cell Biology 85

      Pages: 501-517

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Identification of a protein kinase which phosphorylates a subunit of the 26S proteasome and changes in its activity during meiotic cell cycle in goldfish oocytes.2005

    • Author(s)
      Ryo Horiguchi
    • Journal Title

      Cellular Signaling 17(2)

      Pages: 205-215

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cloning, expression and characterization of three types of 17β-hydroxysteroid dehydrogenases from the Nile tilapia,Oreochromis niloticus.2005

    • Author(s)
      Zhou Lin-Yuen
    • Journal Title

      Journal of Molecular Endocrinology 35(1)

      Pages: 103-116

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Purification and characterization of blarinasin, a new tissue kallikrein-like protease from the short-tailed shrew Blarina brevicauda-comparative studies with blarina toxin.2005

    • Author(s)
      Masaki Kita
    • Journal Title

      Biological Chemistry 386(2)

      Pages: 177-182

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Identification of a protein kinase which phosphorylates a subunit of the 26S proteasome and changes in its activity during meiotic cell cycle in goldfish oocytes.2005

    • Author(s)
      Horiguchi R., Yoshikuni M., Tokumoto M., Nagahama Y., Tokumoto T.
    • Journal Title

      Cellular Signaling 17(2)

      Pages: 205-215

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cloning, expression and characterization of three types of 17β-hydroxysteroid dehydrogenases from the Nile tilapia, Oreochromis niloticus.2005

    • Author(s)
      Zhou LY, Wang DS, Senthilkumaran B, Yoshikuni M, Shibata Y, Kobayashi T, Sudhakumari CC, Nagahama Y.
    • Journal Title

      J Molecular Endocrinology 35(1)

      Pages: 103-116

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Purification and characterization of blarinasin, a new tissue kallilrein-like protease from the short-tailed shrew Biarina brevicauda -comparative studies with blarina toxin.2005

    • Author(s)
      Kita M., Okumura Y, Ohdachi S., Oba Y., Yoshikuni M., Nakamura Y., Kido H., Uemura D.
    • Journal Title

      Biological Chemistry 386(2)

      Pages: 177-182

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A shift in steroidogenesis occurring in ovarian follicles prior to oocyte maturation.2004

    • Author(s)
      Balasubramanian Senthilkumaran
    • Journal Title

      Molecular and Cellular Endocrinology 215(1-2)

      Pages: 11-18

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Blarina Toxin, A Novel Mammalian Lethal Venom from the Short-Tailed Shrew Brevicauda : Isolation and Characterization.2004

    • Author(s)
      Masaki Kita
    • Journal Title

      Proceedings of the National Academy of Science,USA 101(20)

      Pages: 7542-7547

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Inhibitory effect of a SALMFamide neuropeptide on secretion of gonad-stimulating substance from radial nerves in the starfish Aterina pectinifera.2004

    • Author(s)
      Masatoshi Mita
    • Journal Title

      Zoological Science 21(3)

      Pages: 299-303

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A shift in steroidogenesis occurring in ovarian follicles prior to oocyte maturation.2004

    • Author(s)
      Senthilkumaran B., Yoshikuni M., Nagahama Y.
    • Journal Title

      Molecular and Cellular Endocrinology 215(1-2)

      Pages: 11-18

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Inhibitory effect of a SALMFamide neuro-peptide on secretion of gonad-stimulating substance from radial nerves in the starfish Asterina pectinifera.2004

    • Author(s)
      Mita M., Oka H., Thorndyke M.C., Shibata Y., Yoshikuni M., Nagahama Y.
    • Journal Title

      Zoological Science 21(3)

      Pages: 299-303

    • NAID

      110006281378

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Blarina Toxin, A Novel Mammalian Lethal Venom from the Short-Tailed Shrew Blarina Brevicauda : Isolation and Characterization.2004

    • Author(s)
      Kita M., Nakamura Y., Okumura Y., Ohdachi S.D., Oba Y., Yoshikuni M., Kido H., Uemura D.
    • Journal Title

      Proceedings of the National Academy of Science, USA 101(20)

      Pages: 7542-7547

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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