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Transmission of coupling energy from catalytic site to Ca^<2+> transport sites in endoplasmic reticulum calcium pump

Research Project

Project/Area Number 16570091
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionAsahikawa Medical College

Principal Investigator

DAIHO Takashi  Asahikawa Medical College, Biochemistry, Assistant Professor, 医学部, 助教授 (90207267)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordssarcoplasmic reticulum / Ca^<2+>-ATPase / phosphoenzyme / active transport / SERCA / calcium pump / P-type ATPase / Darier Disease / Sarcoplasmic Reticulum / Ca2+-ATPase / Hydrophobic Interaction / Ca2+ transport / Calcium pump / phosphoenzym
Research Abstract

Sarco(endo)plasmic reticulum Ca^<2+>-ATPase (SERCA) transport Ca^<2+> ions from cytoplasm into lumen coupled with ATP hydrolysis, and has essential roles in Ca^<2+> homeostasis. The Ca^<2+> transport sites are located in transmembrane domain, while catalytic site is in the three cytoplasmic domains (A,P, and N). P-domain is phosphorylated with ATP bound to N-domain. Biochemical studies on the intermediates and their analogs indicated that the large rotation of A-domain and its tight association with P-domain occur during the conformational transition of the phosphorylated intermediate (E1P to E2P) and Ca^<2+> release. 1.The author found that Tyr^<122> on the loop connecting A-domain and the 2^<nd> transmembrane helix and other hydrophobic six residues are critical for the hydrophobic interactions between A- and P-domains in the transition and hydrolysis of E2P. 2.The structural natures of stable analogs for E2P of Ca^<2+>-ATPase, i.e. E2BeF,E2A1F, and E2MgF, were explored and compared with actual E2P. It was suggested that the change in hydrophobic nature around phosphorylation site is associated with the change in phosphate geometry from BeF_<3^-> to AlF_3 or AlF_<4^-> and further to MgF_<4^<2->>. Such change likely rearranges transmembrane helices to prevent leakage of lumenal Ca^<2+>. 3.Possible functional abnormalities in three different Darier disease-causing SERCA2b mutants, I274V,L321F, and M719I. Essentially normal expression levels and only slightly reduced Ca^<2+> transport activities of I274V and M719I as compared with wild type suggest that physiological requirement for Ca^<2+> homeostasis in keratinocytes to avoid haploinsufficiency is very strict. The insensitivity to lumenal Ca^<2+> in L321F could possibly be associated with the neuropsychiatric disorder in the pedigee.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (21 results)

All 2006 2005 2004

All Journal Article (21 results)

  • [Journal Article] ATP2C1 is specifically localized in the basal layer of normal epidermis and its depletion triggers keratinocyte differentiation2006

    • Author(s)
      Masaki Yoshida
    • Journal Title

      J Dermatol Sci (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] ATP2C1 is specifically localized in the basal layer of normal epidermis and its depletion triggers keratinocyte differentiation2006

    • Author(s)
      Yoshida, M. et al.
    • Journal Title

      J.Dermatol.Sci. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Critical Hydrophobic Interactions between Phosphorylation and Actuator Domains of Ca^<2+>-ATPase for Hydrolysis of Phosphorylated Intermediate2005

    • Author(s)
      Guoli Wang
    • Journal Title

      The Journal of Biological Chemistry 280・28

      Pages: 26508-26516

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Ca^<2+> occlusion and de-occlusion at transport sites of phosphoenzyme intermediates of sarcoplasmic reticulum Ca^<2+>-ATPase studied by mutagenesis2005

    • Author(s)
      Takashi Daiho
    • Journal Title

      生化学 77・8

      Pages: 803-803

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Analysis of Ca^<2+>-release process in the phosphorylated intermediates of sarcoplasmic reticulum Ca^<2+>-ATPase by mutagenesis2005

    • Author(s)
      Kazuo Yamasaki
    • Journal Title

      生化学 77・8

      Pages: 804-804

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Critical hydrophobic interactions between phosphorylation and actuator domains of Ca^<2+>-ATPase for hydrolysis of phosphorylated intermediate2005

    • Author(s)
      Wang, G et al.
    • Journal Title

      J.Biol.Chem. 280(28)

      Pages: 26508-26516

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Ca^<2+> occlusion and de-occlusion at transport sites of phosphoenzyme intermediates of Sarcoplasmic reticulum Ca^<2+>-ATPase studied by mutagenesis2005

    • Author(s)
      Takashi Daiho, et al.
    • Journal Title

      SEIKAGAKU 77(8)

      Pages: 803-803

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Analysis of Ca^<2+>-release process in the phosphorylated intermediates of sarcoplasmic reticulum Ca^<2+>-ATPase by mutagenesis2005

    • Author(s)
      Kazuo Yamasaki, et al.
    • Journal Title

      SEIKAGAKU 77(8)

      Pages: 804-804

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Distinct type of Abnormality in Kinetic Properties of Three Darier Disease-causing Sarco(endo)plasmic Reticulum Ca2+-ATPase (SERCA2b) Mutants2004

    • Author(s)
      Kazuo Yamasaki
    • Journal Title

      生化学 76・8

      Pages: 781-781

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comprehensive and systematic analysis of function and expression of the 51 missense mutants of SERCA2b found in Darier's Disease patients2004

    • Author(s)
      Takashi Daiho
    • Journal Title

      生化学 76・8

      Pages: 781-781

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Essential Roles of Hydrophobic Interactions at the Interface of Gathered P and A Domains of Sarcoplasmic Reticulum Ca2+-ATPase in Energy Transduction2004

    • Author(s)
      Guoli Wang
    • Journal Title

      生化学 76・8

      Pages: 781-781

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Distinct natures of Be/F-bound, Al/F-bound, and Mg/F-bound stable analogues of an ADP-insensitive phosphoenzyme intermediate of sarcoplasmic reticulum Ca2+-ATPase2004

    • Author(s)
      Hiroshi Suzuki
    • Journal Title

      生化学 76・8

      Pages: 782-782

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Distinct natures of beryllium fluoride-bound, aluminum fluoride-bound, and magnesium fluoride-bound stable analogues of an ADP-insensitive phosphoenzyme intermediate of sarcoplasmic reticulum Ca2+-ATPase : changes in catalytic and transport sites during phosphoenzyme hydrolysis2004

    • Author(s)
      Stefania Danko
    • Journal Title

      The Journal of Biological Chemistry 279・15

      Pages: 14991-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Distinct types of abnormality in kinetic properties of three Darier disease-causing sarco(endo)plasmic reticulum Ca2+-ATPase mutants that exhibit normal expression and high Ca2+ transport activity2004

    • Author(s)
      Katsuhiko Sato
    • Journal Title

      The Journal of Biological Chemistry 279・34

      Pages: 35595-603

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Distinct Natures of Be/F-bound, Al/F-bound, and Mg/F-bound Stable Analogues of ADP-insensitive Phosphoenzyme Intermediate of Sarcoplasmic Reticulum Ca^<2+>-ATPase. CHANGES IN CATALYTIC AND TRANSPORT SITES DURING PHOSPHOENZYME HYDROLYSIS2004

    • Author(s)
      Danko, S. et al.
    • Journal Title

      J.Biol.Chem. 279(15)

      Pages: 14991-14998

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Distinct type of abnormality in kinetic properties of three Darier disease-causing sarco(endo)plasmic reticulum Ca^<2+>-ATPase mutants that exhibit normal expression and high Ca^<2+> transport activity2004

    • Author(s)
      Sato, K. et al.
    • Journal Title

      J.Biol.Chem. 279(34)

      Pages: 35595-35603

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Distinct type of Abnormality in Kinetic Properties of Three Darier Disease-causing Sarco(endo)plasmic Reticulum Ca^<2+>-ATPase (SERCA2b) Mutants2004

    • Author(s)
      Kazuo Yamasaki, et al.
    • Journal Title

      SEIKAGAKU 76(8)

      Pages: 781-781

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comprehensive and systematic analysis of function and expression of the 51 missense mutants of SERCA2b found in Darier's Disease patients2004

    • Author(s)
      Takashi Daiho, et al.
    • Journal Title

      SEIKAGAKU 76(8)

      Pages: 781-781

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Essential Roles of Hydrophobic Interactions at the Interface of Gathered P and A Domains of Sarcoplasmic Reticulum Ca^<2+>-ATPase in Energy Transduction2004

    • Author(s)
      Guoli Wang, et al.
    • Journal Title

      SEIKAGAKU 76(8)

      Pages: 782-782

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Distinct natures of Be/F-bound,Al/F-bound, and Mg/F-bound stable analogues of an ADP-insensitive phosphoenzyme intermediate of Sarcoplasmic reticulum Ca^<2+>-ATPase2004

    • Author(s)
      Hiroshi Suzuki, et al.
    • Journal Title

      SEIKAGAKU 76(8)

      Pages: 782-782

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Distinct type of Abnormality is Kinetic Properties of Three Darier Disease-causing Sarco(endo)plasmic Reticulum Ca2+-ATPase (SERCA2b) Mutants2004

    • Author(s)
      Kazuo Yamasaki
    • Journal Title

      生化学 76・8

      Pages: 781-781

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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