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Development and structural characteristic of apoptosis-inducing α-lactalbumin

Research Project

Project/Area Number 16570129
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionHokkaido University

Principal Investigator

DEMURA Makoto  Hokkaido Univ., Fac. of Adv. Life Sci., Prof, 大学院先端生命科学研究院, 教授 (70188704)

Co-Investigator(Kenkyū-buntansha) AIZAWA Tomoyasu  Hokkaido Univ., Fac. of Adv. Life Sci., Associate Prof, 大学院先端生命科学研究院, 助教授 (40333596)
KAWANO Keiichi  Hokkaido Univ., Fac. of Sci., Prof, 大学院理学研究院, 教授 (10136492)
NITTA Katsutoshi  Hokkaido Univ., Honorary Prof, 名誉教授 (80001858)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsapoptosis / protein / oleic acid / NMR / 脂肪酸 / αラクトアルブミン / キメラ
Research Abstract

α-lactalbumin is a calcium binding protein found in mammalian milk. It is known that α-lactalbumin is one of the components of lactose synthase, which catalyzes the final step in lactose biosynthesis in the lactating mammary gland, and the other component of this system is galactosyltransferase (GT). Most of α-lactalbumin, including human, guinea pig, bovine, goat, camel, equine and rabbit proteins, consist of 123 amino acid residues. Native a-lactalbumin has two structural domains: a large α-helical domain and a small β-sheet domain. The structure is stabilized by four disulfide bridges such as lysozyme. α-lactalbumin forms molten globule state (folding intermediates) at low pH or calcium free state. In this study, development and structural characteristics of the apoptosis-inducing α-lactalbumin as a "non-native" and novel biological function were investigated in detail. A simple and useful method for preparation of non-native structure just like the MG-state which have induction activity of tumor-cell death were developed from not only human but also bovine α-lactalbumin.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (51 results)

All 2007 2006 2005 2004 Other

All Journal Article (49 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Equilibrium and Kinetics of the Folding and Unfolding of Canine Milk Lysozyme2007

    • Author(s)
      H.Nakatani.
    • Journal Title

      Biochemistry (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The solution structure of horseshoe crab antimicrobial peptide tachystatin B with an inhibitory cystine-knot motif2007

    • Author(s)
      Fujitani N.
    • Journal Title

      J. Pept. Sci. 13(4)

      Pages: 269-279

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Structural Approach to a Novel Tandem Repeat DNA-Binding Domain, STPR, by CD and NMR2007

    • Author(s)
      S.Saito.
    • Journal Title

      Biochemistry 46(7)

      Pages: 1703-1713

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Heterologous expression of pharaonis halorhodopsin in Xenopus laevis oocytes and electrophysiological characterization of its light-driven Cl-pump activity, A2007

    • Author(s)
      Seki, S
    • Journal Title

      Biophys. J. 92(7)

      Pages: 2559-2569

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] C. Hasegawa., Interaction of the Halobacterial Transducer to a Halorhodopsin Mutant Engineered so as to Bind the Transducer : Cl-Circulation within the Extracellular Channel2007

    • Author(s)
      C.Hasegawa.
    • Journal Title

      Photochem. Photobiol. 83(2)

      Pages: 293-302

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Destabilization of Transthyretin by Pathogenic Mutations in the DE loop2007

    • Author(s)
      Takeuchi, M.
    • Journal Title

      PROTEINS : Structure, Function, and Bioinformatics 66(3)

      Pages: 716-725

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Structural Approach to a Novel Tandem Repea DNA-Binding Domain, STPR, by CD and NMR2007

    • Author(s)
      S.Saito
    • Journal Title

      Biochemistry 46(7)

      Pages: 1703-1713

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Heterologous expression of pharaonis halorhodopsin in Xenopus laevis oocytes and electrophysiological characterization of its light-driven Cl-pump activity, A2007

    • Author(s)
      Seki, S.
    • Journal Title

      Biophys. J. 92(7)

      Pages: 2559-2569

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Interaction of the Halobacterial Transducer to a Halorhodopsin Mutant Engineered so as to Bind the Transducer : Cl-Circulation within the Extracellular Channel2007

    • Author(s)
      C.Hasegawa.
    • Journal Title

      Photochem. Photobiol. 83(2)

      Pages: 293-302

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Deprotonation of G1u234 during the photocycle of Natronomonas pharaonis halorhodopsin2006

    • Author(s)
      M.Shibata.
    • Journal Title

      Chemical Physics Letters 432(4-6)

      Pages: 545-547

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Differential Scanning Calorimetry of a Metalloprotein under Controlled Metal-Ion Activity2006

    • Author(s)
      M.Yasui.
    • Journal Title

      Protein J. 25(7-8)

      Pages: 475-482

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] N-Terminal Mutational Analysis of the Interaction between Growth-Blocking Peptide (GBP) and Receptor of Insect Immune Cells2006

    • Author(s)
      S.Watanabe.
    • Journal Title

      Protein & Peptide Lett. 13(8)

      Pages: 815-822

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A Novel N14Y Mutation in Connexin26 in KID Syndrome Analyses of Altered Gap Junctional Communication and Molecular Structure of N-Terminus of Mutated Connexin262006

    • Author(s)
      K.Arita.
    • Journal Title

      Am. J Pathology 169(2)

      Pages: 416-423

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Internal Water Molecules of the Proton-Pumping Halorhodopsin in the Presence of Azide2006

    • Author(s)
      N.Muneda.
    • Journal Title

      J. Am. Chem. Soc. 128(19)

      Pages: 6294-6295

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Preparation and Observation of Fresh-frozen Sections of the Green Fluorescent Protein Transgenic Mouse Head2006

    • Author(s)
      M.Tada.
    • Journal Title

      Acta Histochemica et Cytochemica 39(2)

      Pages: 31-34

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Fractionation of silk fibroin hydrolysate and its protective function of hydrogen peroxide toxicity2006

    • Author(s)
      J.-H.Yeo.
    • Journal Title

      J. Appl. Polym. Sci. 102(1)

      Pages: 772-776

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A Novel N14Y Mutation in Connexin26 in KID Syndrome ? Analyses of Altered Gap Junctional Communication and Molecular Structure of N-Terminus of Mutated Connexin262006

    • Author(s)
      K.Arita.
    • Journal Title

      Am. J. Pathology 169(2)

      Pages: 416-423

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Preparation and Observation of Fresh-frozen Sections of the Green Fluorescent Protein Transgenic Mouse Head2006

    • Author(s)
      M.Tada
    • Journal Title

      Acta Histochemica et Cytochemica 39(2)

      Pages: 31-34

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Fractionation of silk fibroin hydrolysate and its protective function of hydrogen peroxide toxicity2006

    • Author(s)
      J-H.Yeo.
    • Journal Title

      J. AppL Polym. Sci. 102(1)

      Pages: 772-776

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Deprotonation of Glu234 during the photocycle of Natronomonas pharaonis halorhodopsin2006

    • Author(s)
      M.Shibata
    • Journal Title

      Chemical Physics Letters 432

      Pages: 545-547

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Differential Scanning Calorimetry of a Metalloprotein under Controlled Metal-Ion Activity2006

    • Author(s)
      M.Yasui
    • Journal Title

      Protein J. 25

      Pages: 475-482

    • Related Report
      2006 Annual Research Report
  • [Journal Article] N-Terminal Mutational Analysis of the Interaction between Growth-Blocking Peptide (GBP) and Receptor of Insect Immune Cells2006

    • Author(s)
      S.Watanabe
    • Journal Title

      Protein & Peptide Lett. 13

      Pages: 815-822

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A Novel N14Y Mutation in Connexin26 in KID Syndrome? Analyses of Altered Gap Junctional Communication and Molecular Structure of N-Terminus of Mutated Connexin262006

    • Author(s)
      K.Arita
    • Journal Title

      Am. J. Pathology 169

      Pages: 416-423

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Internal Water Molecules of the Proton-Pumping Halorhodopsin in the Presence of Azide2006

    • Author(s)
      N.Muneda
    • Journal Title

      J. Am. Chem. Soc. 128

      Pages: 6294-6295

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Preparation and Observation of Fresh-frozen Sections of the Green Fluorescent Protein Transgenic Mouse Head2006

    • Author(s)
      M.Tada
    • Journal Title

      Acta Histochemica et Cytochemica 39

      Pages: 31-34

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Disassembling and bleaching of chloride-free pharaonis halorhodopsin by octyl-beta-glucoside2005

    • Author(s)
      M.Kubo.
    • Journal Title

      Biochemistry 44(39)

      Pages: 12923-12931

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Hydrogen-Bonding Alterations of the Protonated Schiff Base and Water Molecule in the Chloride Pump of Natronobacterium pharaonis2005

    • Author(s)
      M.Shibata.
    • Journal Title

      Biochemistry 44(37)

      Pages: 12279-12286

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Photobleaching of bacteriorhodopsin solubilized with Triton X-1002005

    • Author(s)
      T.Sasaki.
    • Journal Title

      Photochemistry and Photobiology 81(5)

      Pages: 1131-1137

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of the Stabilization of the Molten Globule State on the Folding Mechanism of alpha-Lactalbumin : A Study of a Chimera of Bovine and Human alpha-Lactalbumin2005

    • Author(s)
      M.Mizuguchi.
    • Journal Title

      Proteins 61(2)

      Pages: 356-365

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Role of Putative Anion-Binding Sites in Cytoplasmic and Extracellular Channels of Natronomonas pharaonis Halorhodopsin2005

    • Author(s)
      M.Sato.
    • Journal Title

      Biochemistry 44(12)

      Pages: 4775-4784

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Interaction of dopamine and acetylcholine with an amphiphilic resorcinarene receptor in aqueous micelle system2005

    • Author(s)
      M.Demura.
    • Journal Title

      Bioorganic & Medicinal Chemistry Letters 15(5)

      Pages: 1367-1370

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Disassembling and bleaching of chloride-free pharaonis halorhodopsin by octyl-beta-glucoside2005

    • Author(s)
      M.Kubo
    • Journal Title

      Biochemistry 44

      Pages: 12923-12931

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Hydrogen-Bonding Alterations of the Protonated Schiff Base and Water Molecule in the Chloride Pump of Natronobacterium pharaonis2005

    • Author(s)
      M.Shibata
    • Journal Title

      Biochemistry 44

      Pages: 12279-12286

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Photobleaching of bacteriorhodopsin solubilized with Triton X-1002005

    • Author(s)
      T.Sasaki
    • Journal Title

      Photochemical and Photobiology 81

      Pages: 1131-1137

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effects of the Stabilization of the Molten Globule State on the Folding Mechanism of alpha-Lactalbumin : A Study of a Chimera of Bovine and Human alpha-Lactalbumin2005

    • Author(s)
      M.Mizuguchi
    • Journal Title

      Proteins 61

      Pages: 356-365

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Role of Putative Anion-Binding Sites in Cytoplasmic and Extracellular Channels of Natronomonas pharaonis Halorhodopsin2005

    • Author(s)
      M.Sato
    • Journal Title

      Biochemistry 44

      Pages: 4775-4784

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Interaction of dopamine and acetylcholine with an amphiphilic resorcinarene receptor in aqueous micelle system2005

    • Author(s)
      M.Demura
    • Journal Title

      Bioorganic & Medicinal Chemistry Letters 15

      Pages: 1367-1370

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The Gly-Gly Linker Region of the Insect Cytokine Growth Blocking Peptide (GBP) is Essential for Activity2004

    • Author(s)
      M.Yoshida.
    • Journal Title

      J. Biol. Chem. 279(49)

      Pages: 51331-51337

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effect of Hydrostatic Pressure on Conformational Changes of Canine Milk Lysozyme between the Native, Molten Globule, and Unfolded States2004

    • Author(s)
      M.Watanabe.
    • Journal Title

      Biochim. Biophys. Acta 1702(2)

      Pages: 129-136

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Internal Water Molecules of Light-Driven Chloride Pump Proteins2004

    • Author(s)
      M.Shibata.
    • Journal Title

      Chem. Phys. Lett. 392(4-6)

      Pages: 330-333

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A Non-Native alpha-Helix Is Formed in the beta-Sheet Region of the Molten Globule State of Canine Milk Lysozyme2004

    • Author(s)
      M.Watanabe.
    • Journal Title

      Protein J. 23(5)

      Pages: 335-342

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Volumetric behavior of the molten globule state of canine milk lysozyme2004

    • Author(s)
      M.Watanabe.
    • Journal Title

      Protein Pept. Lett. 11(4)

      Pages: 325-330

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Volumetric behavior of the molten globule Istate of canine milk lysozyme2004

    • Author(s)
      M.Watanabe.
    • Journal Title

      Protein Pept. Lett. 11(4)

      Pages: 325-330

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The Gly-Gly Linker Region of Insect Cytokine Growth Blocking Peptide (GBP) is Essential for Activity2004

    • Author(s)
      M.Yoshida, T.Aizawa, T.Nakamura, K.Shitara, Y.Hayakawa, K.Matsubara, K.Miura, T.Kouno, K.D.Clark, M.R.Strand, M.Mizuguchi, M.Demura, K.Nitta K.Kawano
    • Journal Title

      J.Biol.Chem. 279

      Pages: 51331-51337

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effect of Hydrostatic Pressure on Conformational Changes of Canine Milk Lysozyme between the Native, Molten Globule, and Unfolded States2004

    • Author(s)
      M.Watanabe, T.Aizawa, M.Demura, K.Nitta
    • Journal Title

      Biochim.Biophys.Acta 1702

      Pages: 129-136

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Internal Water Molecules of Light-Driven Chloride Pump Proteins2004

    • Author(s)
      M.Shibata, N.Muneda, K.Ihara, T.Sasaki, M.Demura, H.Kandori
    • Journal Title

      Chem.Phys.Lett. 392

      Pages: 330-333

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A Non-Native alpha-Helix Is Formed in the beta-sheet Region of the Molten Globule State of Canine Milk Lysozyme2004

    • Author(s)
      M.Watanabe, Y.Kobashigawa, T.Aizawa, M.Demura, K.Nitta
    • Journal Title

      Protein J. 23

      Pages: 335-342

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Volumetric behavior of the molten globule state of canine milk lysozyme2004

    • Author(s)
      M.Watanabe, Y.Kobashigawa, T.Aizawa, M.Demura, K.Nitta
    • Journal Title

      Protein Pept Lett. 11

      Pages: 325-330

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Equilibrium and Kinetics of the Folding and Unfolding of Canine Milk Lysozyme

    • Author(s)
      H.Nakatani.
    • Journal Title

      Biochemistry (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] NMR insight of structural stability and folding of calcium-binding lysozyme, Modern Magnetic Resonance, Part I Application in Chemistry, Biological and Marine Sciences.2006

    • Author(s)
      M.Demura(分担)
    • Total Pages
      961
    • Publisher
      Springers
    • Related Report
      2006 Annual Research Report
  • [Patent(Industrial Property Rights)] 腫瘍細胞死誘導物質の製造方法2006

    • Inventor(s)
      出村誠 他3
    • Industrial Property Rights Holder
      北海道大学
    • Industrial Property Number
      2006-024102
    • Filing Date
      2006-02-01
    • Related Report
      2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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