| Project/Area Number |
16570148
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | University of Tsukuba (2005)
Saitama Medical University (2004) |
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Principal Investigator |
HISATAKE Koji University of Tsukuba, Graduate School of Comprehensive Human Sciences, Professor, 大学院・人間総合科学研究科, 教授 (70271236)
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| Co-Investigator(Kenkyū-buntansha) |
NAKADAI Tomoyoshi Saitama Medical School, Department of Medicine, Research associate, 医学部, 助手 (10364770)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | transcription factor / coactivator / TFIID / baculovirus / activator / basal transcriptional machinery / バキュロウイルス |
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| Research Abstract |
We have studied on the transcriptional activity of ILF2/ILF3. The primary structures of ILF2/ILF3 suggested that these factors might bind double-stranded RNA and be involved in the defense against viral infection. We performed a detailed analysis of the interaction of ILF3 with PKR and found that while ILF3 bound the phosphorylated form of PKR, it failed to interact with non-phosphorylated form of PKR. We also found that double-stranded RNA reduced the interactions of ILF3 with activators including SRF, Elk-1, ATF1 and CREB. The reduction in the interactions was not observed with the mutant ILF3 that lacked dsRNA-binding ability, indicating the direct requirement of dsRNA binding in reducing the interactions. Together, the results suggested that the altered transcription of the c-fos gene by dsRNA involve dynamic changes in the complex formation between ILF3/ILF2 and the activators. Furthermore, in vitro transcription studies showed that dsRNA attenuated transcription of the c-fos gene, indicating a negative feedback effect on c-fos transcription by its own transcript. Interaction studied revealed novel interactions between ILF2/ILF3 and PRMT1, PRMT2 and PRMT5. As these proteins methylate arginine residues in proteins, the presence of these interactions supported a view that transcription of the c-fos gene is regulated by methylation of arginine residued with the histone molecules.
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