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Analysis of cellular function of the cytosolic chaperonin CCT composed of eight different subunits

Research Project

Project/Area Number 16570157
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionKyoto University

Principal Investigator

KUBOTA Hiroshi  Kyoto Univ., Inst.Frontier Med.Sci., Assistant professor, 再生医科学研究所, 助手 (80332724)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsCCT / molecular chaperone / protein folding / RNAi / actin / tubulin / aggregation / HSP60 family
Research Abstract

Molecular chaperones play crucial roles in the folding of newly synthesized proteins and the refolding of denatured proteins in the cell. The chaperonin containingt-complex polypeptide 1 (CCT) is a molecular chaperone that facilitates protein folding in eukaryotic cytosol. Although in vitro studies have demonstrated that CCT is required for the folding of proteins including actin and tubulin, roles of CCT in the folding of these proteins in vivo are largely unknown particularly for in mammalian cells. We established RNAi knockdown system to deplete CCT subunits in cultured cells. Microscopic analysis of actin and tubulin labeled with green fluorescent protein (GFP) orits yellow variant (YFP) in the presence of CCT knock-down vector indicated that CCT knockdown results in the aggregation of GEP-tubulin and YFP-actin. These results indicate that CCT prevents aggregate formation of actin and tubulin in vivo in mammalian cells and thus plays an important role for productive folding of these proteins.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (29 results)

All 2006 2005 2004

All Journal Article (28 results) Book (1 results)

  • [Journal Article] Type I collagen in Hsp47-null cells is aggregated in ER and deficient in N-propeptide processing and fibrillogenesis2006

    • Author(s)
      Ishida et al.
    • Journal Title

      Molecular Biology of the Cell 2346・5

      Pages: 2346-2355

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Autoantibodies against HSP70 family proteins were detected in the cerebrospinal fluid from patients with multiple sclerosis2006

    • Author(s)
      Chiba et al.
    • Journal Title

      Journal of Neurological Science 241・1-2

      Pages: 39-43

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Autoantibodies against HSP70 family proteins were detected in the cerebrospinal fluid from patients with multiple sclerosis.2006

    • Author(s)
      Chiba et al.
    • Journal Title

      Journal of Neurological Science 241

      Pages: 39-43

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Type I collagen in Hsp47-null cells is aggregated in ER and deficient in N-propeptide processing and fibrillogenesis.2006

    • Author(s)
      Ishida et al.
    • Journal Title

      Molecular Biology of the Cell 17

      Pages: 2346-2355

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Type I collagen in Hsp47-null cells is aggregated in ER and deficient in N-propeptide processing and fibrillogenesis2006

    • Author(s)
      Ishida et al.
    • Journal Title

      Molecular Biology of the Cell (印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Insufficient folding of type IV collagen and formation of abnormal basement membrane-like structure in embryoid bodies derived from Hsp47-null ES cells2005

    • Author(s)
      Matsuoka et al.
    • Journal Title

      Molecular Biology of the Cell 15・10

      Pages: 4467-4475

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Proinsulin lacking the A7-B7 disulfide bond, Ins2 Akita, tends to aggregate due to the exposed hydrophobic surface2005

    • Author(s)
      Yoshinaga et al.
    • Journal Title

      Biological Chemistry 386・11

      Pages: 1077-1085

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Gene expression in human keloids is altered from dermal to chondrocytic and osteogenic lineage2005

    • Author(s)
      Naitoh et al.
    • Journal Title

      Genes to Cells 10・11

      Pages: 1081-1091

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Octamer and Sox Elements Are Required for Transcriptional cis Regulation of Nanog Gene Expression2005

    • Author(s)
      Kuroda et al.
    • Journal Title

      Molecular and Cellular Biology 25・6

      Pages: 2475-2485

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Octamer and Sox Elements Are Required for Transcriptional cis Regulation of Nanog Gene Expression.2005

    • Author(s)
      Kuroda et al.
    • Journal Title

      Molecular and Cellular Biology 25

      Pages: 2475-2485

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Proinsulin lacking the A7-B7 disulfide bond, Ins2 Akita, tends to aggregate due to the exposed hydrophobic surface.2005

    • Author(s)
      Yoshinaga et al.
    • Journal Title

      Biological Chemistry 386

      Pages: 1077-1085

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Gene expression in human keloids is altered from dermal to chondrocytic and osteogenic lineage.2005

    • Author(s)
      Naitoh et al.
    • Journal Title

      Genes to Cells 10

      Pages: 1081-1091

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Proinsulin lacking the A7-B7 disulfide bond, Ins2 Akita, tends to aggregate due to the exposed hydrophobic surface2005

    • Author(s)
      Yoshinaga et al.
    • Journal Title

      Biological Chemistry 386

      Pages: 1077-1085

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Gene expression in human keloids is altered from dermal to chondrocytic and osteogenic lineage2005

    • Author(s)
      Naitoh et al.
    • Journal Title

      Genes to Cells 10

      Pages: 1081-1091

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Autoantibodies against HSP70 family proteins were detected in the cerebrospinal fluid from patients with multiple sclerosis2005

    • Author(s)
      Chiba et al.
    • Journal Title

      Journal of Neurological Science 241

      Pages: 39-43

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Octamer and Sox elements are required for transcriptional cis-regulation of Nanog gene expression2005

    • Author(s)
      Kuroda et al.
    • Journal Title

      Molecular and Cellular Biology 25・6

      Pages: 2475-2485

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Prevalence of anti-heat shock protein antibodies in cerebrospinal fluids of patients with Guillain-Barre syndrome2004

    • Author(s)
      Yonekura et al.
    • Journal Title

      Journal of Neuroimunology 156・1-2

      Pages: 204-209

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The endoplasmic reticulum stress response is stimulated through the continuous activation of transcription factors ATF6 and XBP1 in Ins2^<+/Akita> pancreatic β cells2004

    • Author(s)
      Nozaki et al.
    • Journal Title

      Genes to Cells 9・3

      Pages: 261-270

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] ZNF143 activates gene expression in response to DNA damage and binds to cisplatin-modified DNA2004

    • Author(s)
      Ishiguchi et al.
    • Journal Title

      Internatinal Journal of Cance 11・6

      Pages: 900-909

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Accumulation of type IV collagen in dilated ER leads to apoptosis in Hsp47-knockout mouse embryos via induction of CHOP2004

    • Author(s)
      Marutani et al.
    • Journal Title

      Journal of Cell Science 117・24

      Pages: 5913-5922

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] The endoplasmic reticulum stress response is stimulated through the continuous activation of transcription factors ATF6 and XBP1 in Ins2^<+/Akita> pancreatic β cells.2004

    • Author(s)
      Nozaki et al.
    • Journal Title

      Genes to Cells 9

      Pages: 261-270

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Prevalence of anti-heat shock protein antibodies in cerebrospinal fluids of patients with Guillain-Barre syndrome.2004

    • Author(s)
      Yonekura et al.
    • Journal Title

      Journal of Neuroimmunology 156

      Pages: 204-209

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Insufficient folding of type IV collagen and formation of abnormal basement membrane-like structure in embryoid bodies derived from Hsp47-null ES cells.2004

    • Author(s)
      Matsuoka et al.
    • Journal Title

      Molecular Biology of the Cell 15

      Pages: 4467-4475

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] ZNF143 activates gene expression in response to DNA damage and binds to cisplatin-modified DNA.2004

    • Author(s)
      Ishiguchi et al.
    • Journal Title

      International Journal of Cancer 11

      Pages: 900-909

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Accumulation of type IV collagen in dilated ER leads to apoptosis in Hsp47-knockout mouse embryos via induction of CHOP.2004

    • Author(s)
      Marutani et al.
    • Journal Title

      Journal of Cell Science 117

      Pages: 5913-5922

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The Endoplasmic reticulum stress response is stimulated through the continuous activation of transcription factors ATF6 and XBP1 in Ins2^<+/Akita> pancreatic β cells2004

    • Author(s)
      Nozaki et al.
    • Journal Title

      Genes to Cells 9・3

      Pages: 261-270

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Prevalence of anti-heat shock protein antibodies in cerebrospinal fluids of patients with Guillain-Barre syndrome2004

    • Author(s)
      Yonekura et al.
    • Journal Title

      Journal of Neuroimmunology 156・1-2

      Pages: 204-209

    • Related Report
      2004 Annual Research Report
  • [Journal Article] ZNF143 activates gene expression in response to DNA damage and binds to cisplatin-modified DNA2004

    • Author(s)
      Ishiguchi et al.
    • Journal Title

      International Journal of Cancer 11・6

      Pages: 900-909

    • Related Report
      2004 Annual Research Report
  • [Book] ティッシュエンジニアリング20062006

    • Author(s)
      田畑泰彦, 岡野光夫編
    • Total Pages
      228
    • Publisher
      日本医学館
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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