• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of Signal Transducing Mechanism in Platelet-Analysis using Proteomics and Cell-permeable Reagents

Research Project

Project/Area Number 16570163
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionKurume University

Principal Investigator

MOROI Masaaki  Kurume University, Institute of Life Science, 分子生命科学研究所, 教授 (00049074)

Co-Investigator(Kenkyū-buntansha) OHNUMA Masaaki  Kurume University, Institute of Life Science, 分子生命科学研究所, 講師 (20223901)
MIURA Yoshiki  Kurume University, Institute of Life Science, 分子生命科学研究所, 講師 (90279240)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsPlatelet / tyrosine phosphorylation / Rap 1 / GPVI
Research Abstract

1.Analysis of Rap 1 activation in platelets
We developed a method quantitating the activated Rap 1 and measured the amount of the activated Rap 1 in platelets when platelets are activated. We found that the Rap 1 activation is not related to integrin activation as indicated by other studies but rather related to platelet release reaction, especially when platelets were activated through GPVI. We also indicated that Rap 1 activation is mediated by PI 3-kinase-dependent tyrosine phosphorylation. (Thromb.Res.In press)
2.Searching the Rap 1-interacting protein
We are now screening the protein which interact with Rap 1 in platelets. GST-fused active Rap 1(Rap 1V12) or inactive Rap 1 (Rap 1 N17) was prepared from E.coli and conjugated with glutathione-Sepharose. These gels were reacted with platelet lysate and the bound proteins were analyzed by mass spectroscopy after 2D gel electorphoresis. Since cytoskeletal proteins were heavily contaminated to these fractions, we could not identify the specific Rap 1-interacting proteins yet.
3.Introduction of proteins or peptides into platelets
We are searching the method which introduce proteins or peptides into platelets. We tried several methods and found that peptides conjugated with fatty acid would be a best method but still it is difficult to show the specificity.
4.Analyzing the mechanism of GPVI-dependent activation
It is well recognized that collagen binds to GPVI and activates platelets through tyrosine-phosphorylation. However, we found that tyrosine phosphorylation and integrin activation are not well related under certain conditions. We are now analyzing the mechanis of phosphorylation-independent activation.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (19 results)

All 2006 2005 2004 Other

All Journal Article (19 results)

  • [Journal Article] Laminin stimulates spreading of platelets through integrin α6β1-dependent activation of GPVI.2006

    • Author(s)
      Inoue, 0.
    • Journal Title

      Blood 107・4

      Pages: 1405-1412

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Analyzing the mechanism of Rapl activation in platelets : Raplactivation is related to the release reaction mediated through the collagen receptor GPVI.2006

    • Author(s)
      Jung, S.M.
    • Journal Title

      Thromb. Res. (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Laminin stimulates spreading of platelets through integrin α6β1-dependent activation of GPVI2006

    • Author(s)
      Inoue, O. et al.
    • Journal Title

      Blood 107-4

      Pages: 1405-1412

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Laminin stimulates spreading of platelets through integrin α6β 1-dependent activation of GPVI.2006

    • Author(s)
      Inoue, O.
    • Journal Title

      Blood 107・4

      Pages: 1405-1412

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Analyzing the mechanism of Rap1 activation in platelets : Rap1 activation is related to the release reaction mediated through the collagen receptor GPVI.2006

    • Author(s)
      Jung, S.M.
    • Journal Title

      Thromb.Res. (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Von Willebrand factor accelerates platelenet adhesion and thrombusformation on a collagen surface in platelet-reduced blood under flow conditions.2005

    • Author(s)
      Tomokiyo, K.
    • Journal Title

      Blood 105・3

      Pages: 1078-1084

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Thrombopoietin initiates demethylation-based transcription of GP6 during megakaryocyte differentiation.2005

    • Author(s)
      Kanaji, S
    • Journal Title

      Blood 105・10

      Pages: 3888-3892

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Relative antithrombotic effect of soluble GPVI dimer compared with anti-GPVI antibodies in mice.2005

    • Author(s)
      Gruner, S.
    • Journal Title

      Blood 105・4

      Pages: 1492-1499

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] The influence of N-linked glycosylation on the function of platelet glycoprotein VI.2005

    • Author(s)
      Kunicki, T.J.
    • Journal Title

      Blood 106・8

      Pages: 2744-2749

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Von Willebrand factor accelerates platelet adhesion and thrombus formation on a collagen surface in platelet-reduced blood under flow conditions2005

    • Author(s)
      Tomokiyo, K. et al.
    • Journal Title

      Blood 105-3

      Pages: 1078-1084

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Relative antithrombotic effect of soluble GPVI dimer compared with anti-GPVI antibodies in mice2005

    • Author(s)
      Gruner, S. et al.
    • Journal Title

      Blood 105-4

      Pages: 1492-1499

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Thrombopoietin initiates demethylation-based transcription of GP6 during megakaryocyte differentiation2005

    • Author(s)
      Kanaji, S. et al.
    • Journal Title

      Blood 105-10

      Pages: 3888-3892

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The influence of N-linked glycosylation on the function of platelet glycoprotein VI2005

    • Author(s)
      Kunicki, T.J. et al.
    • Journal Title

      Blood 106-8

      Pages: 2744-2749

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Von Willebrand factor accelerates platelet adhesion and thrombus formation on a collagen surface in platelet-reduced blood under flow conditions.2005

    • Author(s)
      Tomokiyo, K.
    • Journal Title

      Blood 105・3

      Pages: 1078-1084

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Thrombopoietin initiates demethylation-based transcription of GP6 during megakaryocyte differentiation.2005

    • Author(s)
      Kanaji, S.
    • Journal Title

      Blood 105・10

      Pages: 3888-3892

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Platelet glycoprotein VI : its structure and function2004

    • Author(s)
      Moroi, M.
    • Journal Title

      Thromb. Res. 114・2

      Pages: 221-233

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Platelet glycoprotein VI : its structure and function2004

    • Author(s)
      Moroi, M et al.
    • Journal Title

      Thromb.Res. 105-2

      Pages: 221-233

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Platelet glycoprotein VI : its structure and function2004

    • Author(s)
      Moroi, M
    • Journal Title

      Thromb. Res. 114・2

      Pages: 221-233

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Analyzing the mechanism of Rap 1 activation in platelets : Rap 1 activation is related to the release reaction mediated through the collagen receptor GPVI

    • Author(s)
      Jung.S.M.et al.
    • Journal Title

      Thromb.Res. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi