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Analysis of Myc-target gene, Mina53 in cells and in a body

Research Project

Project/Area Number 16570164
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionKurume University

Principal Investigator

TSUNEOKA Makoto  Kurume University, Research Center for Innovative Cancer Therapy, Associate Professor, 先端癌治療研究センター, 助教授 (50197745)

Co-Investigator(Kenkyū-buntansha) KODA Yoshiro  Kurume University, Department of Forensic Medicine, Professor, 医学部, 教授 (90231307)
SOEJIMA Mikiko  Kurume University, Department of Forensic Medicine, Assistant, 医学部, 助手 (80279140)
TEYE Kwesi  久留米大学, 医学部, 助手 (80352128)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsOncogene / Cancer / Esophageal Cancer / Testis / Transcription / myc / mina53 / プロモーター / 細胞増殖 / 核小体 / mimitin / ミトコンドリア
Research Abstract

Mina53 is a nuclear protein, which has a domain called JmjC. When the effect of Mina53 over-expression on activity of various promoters, it was found that Mina53 stimulates E2F site dependent promoter activity. Since E2F is a transcription factor, which highly influences cell proliferations, the activity of Mina53 to stimulate the E2F site dependent promoter activity appears to be involved in the control of cell proliferation by Mina53. In 2006, it was reported that JmjC domain is related to Histone demethylase activity. Then it becomes an important next question if Mina53 has the enzyme activity.
We produced antibody that recognized mouse Mina53 protein, and then investigate expression pattern of Mina53 in a mouse body. It was observed the high expression of Mina53 in testis, spleen, colon, where cells are proliferating well. When we investigated the expression pattern of Mina53 in testis in detail, it was found that Mina53 is highly expressed in spermatogonia, especially in type A spe … More rmatogonia.These results suggest that Mina53 is related to cell proliferation not only in cancer but also in normal tissues.
2,Myc-target genes can be classified into two classes
We cloned another Myc-target gene mimitin by the similar methods for mina53 cloning. Mimitin was found to encode Mitochondrial protein and involved in cell proliferation of esophageal squamous cell carcinoma cells. Mina53 and Mimitin are highly expressed in some human cancer tissues, and they are required for cell proliferation in some types of cells. However, it appears some types of cells do not require them for cell proliferation. These may be due to the existence of a functionally redundant protein and/or mechanism. These Myc-target genes can be classified into one group as "specufuc type". On the other hand, there are Myc-responsive genes, which appear to be generally important for all types of cells to proliferate. Inhibition of the function of one of the specific type of Myc-responsive genes may hamper only some restricted types of cells and would have fewer side effects and more potential than a general type for cancer therapy. Less

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (24 results)

All 2006 2005 2004 2003 Other

All Journal Article (21 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Expression of Mina53,product of a Myc-target gene,in mouse testis2006

    • Author(s)
      Tsuneoka, M., et al.
    • Journal Title

      International Journal of Andrology 29

      Pages: 323-330

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Haptoglobin Gene Promoter Polymorphism and Haplotypes are unique in Different Populations2006

    • Author(s)
      Teye, K., et al.
    • Journal Title

      Human Biology in press

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 大腸癌における新規Myc標的遺伝子mina53の発現2006

    • Author(s)
      常岡 誠 ほか1名
    • Journal Title

      Biotherapy (癌と化学療法社) 20・1

      Pages: 105-117

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression of Mina53, product of a Myc-target gene, in mouse testis2006

    • Author(s)
      Tsuneoka, M., et al.
    • Journal Title

      International Journal of Andrology 29

      Pages: 323-330

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Haptoglobin Gene Promoter Polymorphism and Haplotypes are unique in Different Populations2006

    • Author(s)
      Teye, K., et al.
    • Journal Title

      Human Biology (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression of A Novel Myc-target gene mina53 in colon cancer2006

    • Author(s)
      Tsuneoka, M., et al.
    • Journal Title

      Biotherapy 20.1

      Pages: 105-117

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression of Mina53,product of a Myc-target gene, in mouse testis.2006

    • Author(s)
      Tsuneoka, M., et al.
    • Journal Title

      International Journal of Andrology. 29

      Pages: 323-330

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 大腸癌における新規Myc標的遺伝子mina53の発現2006

    • Author(s)
      常岡 誠
    • Journal Title

      Biotherapy(癌と化学療法社) 第20巻第一号

      Pages: 105-117

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A novel Myc-target gene, mimitin, which is involved in cell proliferation of esophageal squamous cell carcinoma2005

    • Author(s)
      Tsuneoka, M., et al.
    • Journal Title

      Journal of Biological Chemistry 280・20

      Pages: 19977-19985

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] GIP stimulation of pancreatic beta-cell survival is dependent upon phosphatidylinositol 3-kinase (PI3-K)/ protein kinase B (PKB) signaling, inactivation of the forkhead transcription factor Foxol and downregulation of bax expression2005

    • Author(s)
      Kim S.J., et al.
    • Journal Title

      Journal of Biological Chemistry 280・23

      Pages: 22297-22307

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A novel Myc-target gene, mimitin, which is involved in cell proliferation of esophageal squamous cell carcinoma2005

    • Author(s)
      Tsuneoka, M., et al.
    • Journal Title

      Journal of Biological Chemistry 280.20

      Pages: 19977-19985

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] GIP stimulation of pancreatic beta-cell survival is dependent upon phosphatidylinositol 3-kinase (PI3-K)/protein kinase B (PKB) signaling, inactivation of the forkhead transcription factor Foxo1 and downregulation of bax expression2005

    • Author(s)
      Kim S.J., et al.
    • Journal Title

      Journal of Biological Chemistry 280.23

      Pages: 22297-22307

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A novel Myc-target gene, mimitin, which is involved in cell proliferation of esophageal squamous cell carcinoma2005

    • Author(s)
      Tsuneoka, M., et al.
    • Journal Title

      Journal of Biological Chemistry 280,(20)

      Pages: 19977-19985

    • Related Report
      2005 Annual Research Report
  • [Journal Article] GIP stimulation of pancreatic beta-cell survival is dependent upon phosphatidylinositol 3-kinase (PI3-K)/protein kinase B (PKB) signaling, inactivation of the forkhead transcription factor Foxol and downregulation of bax expression2005

    • Author(s)
      Kim S.J., et al.
    • Journal Title

      Journal of Biological Chemistry 280,(23)

      Pages: 22297-222307

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Mina53 as a Potential Prognostic Factor for Esophageal Squamous Cell Carcinoma.2004

    • Author(s)
      Tsuneoka, M., et al.
    • Journal Title

      Clinical Cancer Research 10・21

      Pages: 7347-7356

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Mina53 as a Potential Prognostic Factor for Esophageal Squamous Cell Carcinoma.2004

    • Author(s)
      Tsuneoka, M., et al.
    • Journal Title

      Clinical Cancer Research 10.21

      Pages: 7347-7356

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A novel 1247T missense mutation in the haptoglobin 2 β chain decreases the protein expression and associates with ahaptoglobinemia.2004

    • Author(s)
      Teye, K. et al.
    • Journal Title

      Hum.Genet. 114・5

      Pages: 499-502

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Mina53 as a Potential Prognostic Factor for Esophageal Squamous Cell Carcinoma.2004

    • Author(s)
      Tsuneoka, M. et al.
    • Journal Title

      Clin.Cancer Res. 10・21

      Pages: 7347-7356

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Haptoglobin Gene Promoter Polymorphism and Haplotypes are unique in Different Populations

    • Author(s)
      Teye, K., et al.
    • Journal Title

      Human Biology (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Nucleotide Sequence Analyses of Human Complement 6 (C6) Gene Suggest Balancing Selection.

    • Author(s)
      Soejima, M. et al.
    • Journal Title

      Annals of Human Genetics (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A novel Myc-target gene, mimitin, which is involved in cell proliferation of esophageal squamous cell carcinoma.

    • Author(s)
      Tsuneoka, M. et al.
    • Journal Title

      J.Biol.Chem. (In press)

    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] Myc標的遺伝子mimitin2004

    • Inventor(s)
      常岡 誠
    • Industrial Property Rights Holder
      久留米大学
    • Industrial Property Number
      2004-147964
    • Filing Date
      2004-05-18
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Patent(Industrial Property Rights)] c-Myc標的遺伝子2004

    • Inventor(s)
      常岡 誠
    • Industrial Property Rights Holder
      久留米大学
    • Industrial Property Number
      2004-147964
    • Filing Date
      2004-05-18
    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] がん関連遺伝子 mina53、蛋白質 Mina53 およびそのモノクロナール抗体2003

    • Inventor(s)
      常岡 誠, 木村博司
    • Industrial Property Rights Holder
      久留米大学
    • Filing Date
      2003-04-14
    • Acquisition Date
      2005-12-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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