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A study of the transition mechanism in embryonic cell cycles with translucent Xenopus blastomeres

Research Project

Project/Area Number 16570175
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionYamaguchi University

Principal Investigator

IWAO Yasuhiro  Yamaguchi University, Faculty of Science, Professor, 理学部, 教授 (10144916)

Co-Investigator(Kenkyū-buntansha) UENO Shuichi  Yamaguchi University, Faculty of Science, Research assistant, 理学部, 助手 (80363092)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsCleavage / Cell cycle / MBT / Amphibians / MPF / PCNA / Microtubules / Translucent blastomere / DNA合成 / Caイオン濃度
Research Abstract

In order to clarify the mechanism of the elongation and asynchrony of cell cycles after Midblastula transition (MBT), the translucent blastomeres of the frog, Xenopus laevis, were produced by centrifugation and developed in Ca^<2+>-free medium. Many translucent blatomeres were isolated around the 8th cell cycle. To measure the increase in DNA content in each blastomere without any deleterious effect, the blastomeres were treated with a vital DNA-staining fluorescence dye and the fluorescence was measured under the exposure of very weak UV light with very short period. We found G1,S,G2,M phases in each cell cycle based on the change in DNA fluorescence in the nucleus. At first, S and M phases were elongated, and then the cell cycle length greatly increased by the addition on G2 and G1 phases in this order. To inject mRNA of EGFP-PCNA into fertilized eggs, the behavior of PCNA during cell cycles were observed in the live cleaving blastomres. To inject a Ca^<2+>-sensitive fluorescence dye into fertilized eggs, the change in Ca^<2+> concentration during cleavage was measured in live translucent blastomeres. The Ca^<2+> concentration increased just before M phase. Thus, we have developed a novel system to study the behavior of molecules important for cell cycle regulation in live blastomeres during early embryonic cell cycle.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (8 results)

All 2005 Other

All Journal Article (8 results)

  • [Journal Article] The cytoskeleton-dependent localization of cdc2/cyclin B in blastomere cortex during Xenopus embryonic cell cycle2005

    • Author(s)
      N.Nakamura, T.Tokumoto, S.Ueno, Y.Iwao
    • Journal Title

      Molecular Reproduction and Development 72

      Pages: 336-345

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Midblastula Transition (MBT) of the cell cycles in the yolk and pigment granules-free translucent blastomeres obtained from centrifuged Xenopus embryos2005

    • Author(s)
      Y.Iwao, Y.Uchida, S.Ueno, N.Yoshizaki, Y.Masui
    • Journal Title

      Development Growth & Differentiation 47

      Pages: 283-294

    • NAID

      10016623085

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] 目で見る受精過程-受精と卵割2005

    • Author(s)
      岩尾康宏
    • Journal Title

      Hormone Frontier In Gynecology 12

      Pages: 2-5

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Midblastula Transition (MBT) of the cell cycles in the yolk and pigment granules-free translucent blastomeres obtained from centrifuged Xenopus embryos2005

    • Author(s)
      Y.Iwao, Y.Uchida, S.Ueno, N.Yoshizaki, Y.Masui
    • Journal Title

      Development, Growth & Differentiation 47

      Pages: 283-294

    • NAID

      10016623085

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The visible process of fertilization -Fertilization and Cleavage-(in Japanese)2005

    • Author(s)
      Y.Iwao
    • Journal Title

      Hormone Frontier In Gynecology 12

      Pages: 2-5

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The cytoskeleton-dependent localization of cdc2/cyclin Bin blastomere cortex during Xenopus embryonic cell cycle2005

    • Author(s)
      N.Nakamura, T.Tokumoto, S.Ueno, Y.Iwao
    • Journal Title

      Molecular Reproduction and Development 72

      Pages: 336-345

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 目で見る受精過程-受精と卵割-2005

    • Author(s)
      岩尾康宏
    • Journal Title

      Hormone Frontier In Gynecology 12

      Pages: 2-5

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Midblastula Transition (MBT) of the cell cycles in the yolk and pigment granules-free translucent blastomeres obtained from centrifuged Xenopus embryos

    • Author(s)
      Y.Iwao
    • Journal Title

      Development, Growth & Differentiation (印刷中)

    • NAID

      10016623085

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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