| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2006: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
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| Research Abstract |
The development of Agaricus blazei cultivars with superior properties such as high fruiting body productivity will be very important for promoting commercial production. There is, however, little information available about the development of A.blazei cultivars. Therefore, fundamental genetic research on A.blazei takes on additional importance. This study was performed to examine genetic differences between heterokaryotic strains and their single-spore isolates (SSIs) and to confirm hybrid formation by pairing of SSIs based on AFLP profiles. Furthermore, stimulation conditions of the spore germination and de-heterokaryotization by the protoplast regeneration method.
The total number of AFLP (Amplified Fragment Length Polymorphism) bands from individual SSIs was clearly less than those from their parental strains (heterokaryotic strains), indicating that the discrimination of heterokaryotic strain and its SSIs was possible by the AFLP profile. Many of polymorphic AFLP bands from the parental strains segregated in SSIs at a ratio of 1 : 1, suggesting that the SSIs are homokaryotic. Although it was very difficult to confirm hybrid formation by only colony morphologies of the isolates from paired colonies between SSIs, AFLP bands are suitable genetic makers for the confirmation of successful crosses between SSIs in A.blazei. Effects of several factors on basidiospore germination of A.blazei were examined : bark compost-extracts medium as basal medium, addition of succinic acid to medium, co-culture with living mycelia of A.blazei heterokaryotic strain, and heat treatment of basidiospores before culturing would be effective to stimulate spore germination. In addition, protoplast regeneration method seemed to be useful for de-heterokaryotization in A.blazei
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