| Project/Area Number |
16580003
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Kobe University |
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Principal Investigator |
OHMIDO Nobuko Kobe University, Faculty of Human Development, Associate Professor, 発達科学部, 助教授 (30343263)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Chromosome map / Gene Mapping / Legume / Lotus japonicus / Miyakogusa / マッピング / TACクローン / 画像解析 |
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| Research Abstract |
Lotus japonicus, Miyakojima MG-20 is a cytological model plant of the legume family. I have developed quantitative pachytene chromosome maps and combined them with the other Lotus chromosome maps to integrate their information for effective and accurate genome analysis. Pachytene chromosomes applied the quantitative chromosome image analysis and FISH methods using four repetitive sequences and 28 transformation-competent artificial chromosome (TAC) clones are used for higher resolution mapping. The addresses of gene loci had been identified by chromosome image analysis system (CHIAS), which measures the condensation pattern (CP) of the pachytene chromosome. We have integrated three maps ; mitotic prometaphase chromosome map, meiotic pachytene chromosome map and linkage map. The integrated maps demonstrated precise physical positions of TAC clones on chromosomes, and associated with chromosomal higher structures, representing in centromeres, telomeres, cytologically specific heterochromatin and euchromatin. Moreover, we compared genetic distances derived from recombination frequency with physical distances between DNA markers on pachytene chromosome maps.
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