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Screening and characterization of a rice mutant lacking phospholipase D in mature seeds.

Research Project

Project/Area Number 16580006
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

SUZUKI Yasuhiro  National Agriculture and Food Research Organization, National Institute of Crop Science, Rice Quality Team, Team Leader, 作物研究所米品質研究チーム, チーム長 (40370548)

Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsphospholipase D / rice / storage stability / free fatty acids / old rice / lipids / ホスホリパーゼD
Research Abstract

Deterioration of rice grains during the storage is a serious problem which reduces the quality of stored grains, and the degradation of lipids is responsible for these changes during the storage. Phospholipase D (PLD) which catalyses the terminal phosphodiester bonds in various diester glycerophospholipids. In rice bran, PLD would be responsible for the hydrolysis of spherosome (oil body) membrane phospholipids, which causes triacylglycerol leakage out of spherosomes and subsequent degradation. Using anti-PLD antibody, we screened a rice mutant "03-sl08" lacking PLD in mature seeds. Furthermore, the specific activity of "03-s108" was very low, less than one % of that of Nipponbare. The mode of inheritance of PLD-null character was analyzed by crossing "03-s108" with a normal PLD activity "Koshihikari". The distribution of F2 phenotypes for the presence or absence of PLD from selfed Fl plants was 3:1, supporting the hypothesis that the absence of PLD is recessive to the presence of PLD. Thus, we isolated a rice mutant "03-s108" lacking PLD in mature seeds. This mutant "03-s108" should be useful in breeding programs designed to produce rice of a high storability and in understanding physiologic and genetic mechanisms of lipids metabolism in bran fractions.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (28 results)

All 2007 2006 2005 2004 Other

All Journal Article (27 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] オオムギのリポキシゲナーゼ活性簡易評価法及びリポキシゲナーゼ欠失突然変異系統の作出2007

    • Author(s)
      大関美香ら
    • Journal Title

      研究成果情((総合農業)、平成18年度 (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] ビール大麦日本品種のリポキシゲナーゼ活性の変異と新たなLox-1活性欠損突然変異系統の作出2007

    • Author(s)
      大関美香ら
    • Journal Title

      育種学研究 (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] オオムギのリポキシゲナーゼ活性簡易評価法及びリポキシゲナーゼ欠失突然変異系統の作出2007

    • Author(s)
      大関美香ら
    • Journal Title

      研究成果情報(総合農業)、平成18年度 印刷中

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 米のアミロース含量の変動-機構と調節2006

    • Author(s)
      鈴木保宏
    • Journal Title

      農業および園芸 81 (1)

      Pages: 183-190

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Analysis of expression of genes for mitochondrial aldehyde dehydrogenase in maize during submergence and following re-aeration.2006

    • Author(s)
      Meguro, N. et al.
    • Journal Title

      Breed. Sci. 56

      Pages: 365-370

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Fluctuation of amylose content in rice seeds. Its regulation. (In Japanese)2006

    • Author(s)
      Suzuki, Y.
    • Journal Title

      Agriculture and Horticulture. 81

      Pages: 183-190

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Analysis of Expression of Genes for Mitochondrial Aldehyde Dehydrogenase in Maize during Submergence and Following Re-aeration.2006

    • Author(s)
      Meguro, N., H.Tsuji, Y.Suzuki, N.Tsutsumi, A.Hirai, M.Nakazono.
    • Journal Title

      Breed. Sci. 56

      Pages: 365-370

    • NAID

      110004863708

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 米のアミロース含量の変動 -機構と調節2006

    • Author(s)
      鈴木保宏
    • Journal Title

      農業および園芸 81(1)

      Pages: 183-190

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A rice mutant with enhanced amylose content in endosperm derived from a low amylose variety "Snow pearl". Isolation and cha2005

    • Author(s)
      Suzuki, Y. et al.
    • Journal Title

      Proceedings of the World Rice Research Conference, Tsukuba, Japan.

      Pages: 260-261

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A rice mutant showing position-dependent leaf abnormality.2005

    • Author(s)
      Suzuki, Y. et al.
    • Journal Title

      Rice Genetics Newsletter. 21

      Pages: 13-14

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] 胚乳アミロース含量が増大するイネ変異体「SP14」2005

    • Author(s)
      鈴木保宏ら
    • Journal Title

      研究成果情報(総合農業)、平成16年度

      Pages: 38-39

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 低アミロ-ス米品種「たきたて」の精米白度とアミロ-ス含量および水分含量の関係2005

    • Author(s)
      宮野法近, 鈴木保宏
    • Journal Title

      日本作物学会記事 74 (1)

      Pages: 72-75

    • NAID

      110001743031

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 胚乳のアミロペクチン構造に影響せずにアミロース含量が増大する変異体系統「SP14」2005

    • Author(s)
      鈴木保宏
    • Journal Title

      くろっぷニュース 17

      Pages: 2-2

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Correlation among endosperm whiteness, amylose and moisture content of low amylose rice variety Takitate. (In Japanese)2005

    • Author(s)
      Miyano, N., Y.Suzuki.
    • Journal Title

      Jpn. J. Crop Sci. 74

      Pages: 72-75

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A rice mutant "SP14", with enhanced amylose content in endosperm without effecting amylopectin structure. (In Japanese)2005

    • Author(s)
      Suzuki, Y.
    • Journal Title

      Crop News 17:2

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A rice mutant with enhanced amylose content in endosperm derived from a low amylose variety "Snow pearl". Isolation and characterization.2005

    • Author(s)
      Suzuki, Y. et al.
    • Journal Title

      Proceedings of the World Rice Research Conference, Tsukuba Japan

      Pages: 260-261

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 低アミロース米品種「たきたて」の精米白度とアミロース含量および水分含量の関係2005

    • Author(s)
      宮野法近, 鈴木保宏
    • Journal Title

      日本作物学会記事 74(1)

      Pages: 72-75

    • NAID

      110001743031

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A rice mutant showing position-dependent leaf abnormality.2005

    • Author(s)
      Suzuki, Y. et al.
    • Journal Title

      Rice Genetics Newsletter. 21(in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Differences in starch characteristics of rice strains having different sensitivities to maturation temperatures.2004

    • Author(s)
      Suzuki, Y. et al.
    • Journal Title

      J. Agron. Crop Sci. 190(3)

      Pages: 218-221

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] α-アミラーゼ活性の粳米と糯米の比較および糊化粘度への影響2004

    • Author(s)
      松倉 潮ら
    • Journal Title

      日本食品科学工学会誌 51 (10)

      Pages: 554-558

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Differences in starch characteristics of rice strains having different sensitivities to maturation temperatures.2004

    • Author(s)
      Suzuki, Y., Y.Sano, T.Ishikawa, T.Sasaki, U.Matsukura, H.-Y.Hirano.
    • Journal Title

      J. Agron. Crop Sci. 190

      Pages: 218-221

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A rice mutant with enhanced amylose content in endosperm derived from a low amylose variety "Snow pearl". Isolation and characterization.2004

    • Author(s)
      Suzuki, Y, H.-Y.Hirano, Y.Sano, K.Ise, U.Matsukura, N.Aoki, H.Sato.
    • Journal Title

      Proceedings of World Rice Research Conference 2004.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Comparison alpha-amylase activity in rice grain of nonglutinous and glutinous varieties of alpha-amylase activity on rice pasting property. (In Japanese)2004

    • Author(s)
      Matsukura, U., Y.Suzuki, Y.Iwai, M.Momma, N.Aoki, S.Kaneko.
    • Journal Title

      Nippon Shokuhin Kogaku Kaishi. 51

      Pages: 554-558

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A rice mutant showing position-dependent leaf abnormality.2004

    • Author(s)
      Suzuki, Y, H.Hirasawa, R.Ikeda, T.Ishikawa.
    • Journal Title

      Rice Genetics Newsletter. 21

      Pages: 13-14

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Differences in starch characteristics of rice strains having different sensitivities to maturation temperatures.2004

    • Author(s)
      Suzuki, Y. et al.
    • Journal Title

      J.Agron.Crop Sci. 190(3)

      Pages: 218-221

    • Related Report
      2004 Annual Research Report
  • [Journal Article] α-アミラーゼ活性の粳米と糯米の比較および糊化粘度への影響2004

    • Author(s)
      松倉 潮ら
    • Journal Title

      日本食品科学工学会誌 51(10)

      Pages: 554-558

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Genetic variation in lipoxygenase activity among Japanese malting barley cultivars and identification of a new lipoxygenase-1 deficient mutant.

    • Author(s)
      Oozeki, M., T.Nagamine, T.Ikeda, Y.Suzuki, T.Sekiwa, E.Yamaguchi, T.Kato.
    • Journal Title

      Breed. Sci. (in Press)

    • NAID

      110006314044

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] ホスホリパーゼD欠失性イネ系統2007

    • Inventor(s)
      鈴木保宏
    • Industrial Property Rights Holder
      (独)農業・食品産業技術総合研究機構
    • Industrial Property Number
      2007-053214
    • Filing Date
      2007
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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