| Project/Area Number |
16580037
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied entomology
|
|---|
| Research Institution | Tokyo University of Agriculture and Technology |
|---|
Principal Investigator |
HIRAOKA Tsuyoshi Tokyo University of Agriculture and Technology, Institute of Symbiotic Science and Technology, Associate Professor, 大学院共生科学技術研究院, 助教授 (10238339)
|
|---|
| Project Period (FY) |
2004 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
|
|---|
| Keywords | insect culture cell / functional protein / secreted protein |
|---|
| Research Abstract |
Acetylcholinesterase was purified from conditioned medium of insect cell line NIAS-AeA1-2. SDS-PAGE was suggested that molecular weight was 67kDa, and gel-filtrated chromatography suggested this protein was dimmer in conditioned medium.
Growth blocking peptide, an insect EGF type growth factor was detected by RT-PCR in SES-NaBr-4 and SES-NaBr-93 cell line.
Trypsin inhibitor was detected in NIAS-AeA1-2, and SES-MaBr-4. I tried to purify this protein but in step of affinity column chromatography, this protein adsorbed tightly to trypsin. This protein still unclear about molecular weight and function.
Dopa-decalboxilase was detected by RT-PCR in SES-NaBr-4 Cell line
|
