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Genetic analyses of response mechanisms for aluminum stress in plant

Research Project

Project/Area Number 16580046
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionOkayama University

Principal Investigator

EZAKI Bunichi  Okayama University, Research Institute for Bioresources, Associate Professor, 資源生物科学研究所, 助教授 (90243500)

Co-Investigator(Kenkyū-buntansha) NAKASHIMA Susumu  Research Institute for Bioresources, Associate Professor, 資源生物科学研究所, 助教授 (60033122)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsaluminum (Al) stress / gene response mechanism / Al stress induced gene / transcription factor / glutathione S-transferase / oxidative stress / signal transduction / Arabidopsis thaliana / Ring Zinc finger protein / Homeobox-Leucine Zipper / 転写調節因子 / アルミニウム(Al)ストレス / ゲルシフトアッセイ / Bio-panning / AtGST11遺伝子 / 遺伝子発現応答機構 / aluminum(Al)stress / glutathione S-transferase (GST)
Research Abstract

1. Construction of cDNA library to isolate and characterize several candidate clones encoding transcription factor(s) which are related for the gene expression of the AtGST11 gene under aluminum (Al) stress.
Using mRNA molecules derived from Al treated Arabidopsis thaliana, cDNA library was constructed. The constracted cDNA library were applied for a 3 times repeated screening designated "Biopanning". Finally, we could isolate 50 candidates clones in the third screening.
2. Molecular genetical analysis of the isolated clones
Isolated 50 clones were applied to DNA sequencing and could be classified into 8 genes. Ring Zn finger protein (#13) and Homeobox-Leucine Zipper protein 6 (#43) which have been already reported as transcription factors were included in these clones. One clone (#4) which encodes an unknown protein was also included. Unfortunately, other 5 genes seemed not to be related to gene-expression of the AtGST11 gene.
3. Gel shift assay
To confirm whether the three clones (#4, 13 and 43) actually can bind to the promoter region of the AtGST11 gene, gel shift assay was performed using the purified three proteins. These proteins were extracted and purified by His-tag affinity column before the gel shift assay. The result suggested that all of these proteins can bind to the promoter.
4. New approach (One hybrid assay) for an isolation of transcription factors
In the last year of this project (2006), we also started a new approach to isolate transcription factor(s) which are related to the gene-expression of either the AtGST1 or AtGST11 gene under Al stress by "Yeast One Hybrid System"., We could isolate four new candidates from this screening.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (21 results)

All 2007 2006 2005 2004

All Journal Article (21 results)

  • [Journal Article] Over-expression of an auxilin-like gene (F9E10.5) can suppress Al uptake in roots of Arabidopsis.2007

    • Author(s)
      B.Ezaki, H.Kiyohara, H.Matsumoto, S.Nakashima
    • Journal Title

      J. Exp. Botany 58

      Pages: 497-506

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] かび臭物質生産ラン藻Oscillatoria brevisの重金属ストレスに対する適応機構2007

    • Author(s)
      廣瀬和信, 江崎文一, 下石靖昭, 村田芳行, 中島 進
    • Journal Title

      分取クロマトグラフィー研究会誌 1・3

      Pages: 16-25

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Molecular mechanism on adaptation against heavy metal stress in the musty-order producing cyanobacterium Oscillatoria brevis2007

    • Author(s)
      K.Hirose, B.Ezaki, Y.Shimoishi, Y.Murata, S.Nakashima
    • Journal Title

      J. Preparative Chromatography Association (Japanese) 1-3

      Pages: 16-25

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Diamide stress induces a methalothioneine BmtA through a repressor BxmR and is modulated by Zn-inducible BmtA in the cyanobacterium Oscillatoria brevis2006

    • Author(s)
      K.Hirose, B.Ezaki, T.Liu, S.Nakashima
    • Journal Title

      Toxicology Letters. 163

      Pages: 250-256

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Diamide stress induces a methalothioneine BmtA through a repressor BxmR and is modulated by Zn-inducible BmtA in the cyanobacterium Oscillatoria brevis2006

    • Author(s)
      K.Hirose, B.Ezaki, T.Liu, S.Nakashima
    • Journal Title

      Toxicology Letters 163

      Pages: 250-256

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Diamide stress induces a metallothionein BmtA through a repressor BxmR and is modulated by Zn-inducible BmtA in the cyanobacterium Oscillatoria brevis.2006

    • Author(s)
      Hirose, K., Ezaki, B., Liu, T., Nakashima, S.
    • Journal Title

      Toxicology Letters (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Functions of two aluminum (Al) stress resistance : repression of oxidative damage by the AtBCB gene and promotion of efflux of Alions by the NtGDIl gene.2005

    • Author(s)
      Ezaki, B., Sasaki, K., Matsumoto, H., Nakashima, S.
    • Journal Title

      J. Exp. Botany 56

      Pages: 2661-2671

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Salt stress-induced lipid peroxidation is reduced by glutathione S-transferase, but this reduction of lipid peroxides is not enough for a recovery of root growth in Arabidopsis.2005

    • Author(s)
      Katsuhara, M., Otsuka, T., Ezaki, B.
    • Journal Title

      Plant Science 169

      Pages: 369-373

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Lygodium japonicum fern accumulates copper in the cell wall pectin.2005

    • Author(s)
      Konno, K., Nakato, T., Nakashima, S., Katoh, K.
    • Journal Title

      J. Exp. Botany 56

      Pages: 1923-1931

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Functions of two aluminum (Al) stress resistance : repression of oxidative damage by the AtBCB gene and promotion of efflux of Al ions by the NtGDI1 gene.2005

    • Author(s)
      Ezaki B., Sasaki K., Matsumoto H., Nakashima S.
    • Journal Title

      J. Exp. Botany 56

      Pages: 2661-2671

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Salt stress-induced lipid peroxidation is reduced by glutathione S-transferase, but this reduction of lipid peroxides is not enough for a recovery of root growth in Arabidopsis.2005

    • Author(s)
      Katsuhara M., Otsuka T., Ezaki B.
    • Journal Title

      Plant Science 169

      Pages: 369-373

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Lygodium japonicum fern accumulates copper in the cell wall pectin.2005

    • Author(s)
      Konno K., Nakato T., Nakashima S., Katoh K.
    • Journal Title

      J. Exp. Botany 56

      Pages: 1923-1931

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Functions of two aluminum (Al) stress resistance : repression of oxidative damage by the AtBCB gene and promotion of efflux of Al ions by the NtGDI1 gene.2005

    • Author(s)
      Ezaki, B., Sasaki, K., Matsumoto, H., Nakashima, S.
    • Journal Title

      J.Exp.Botany 56

      Pages: 2661-2671

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Lygodium japonicum fern accumulates copper in the cell wall pectin.2005

    • Author(s)
      Konno, K., Nakato, T., Nakashima, S., Katoh, K.
    • Journal Title

      J.Exp.Botany 56

      Pages: 1923-1931

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Mechanism of gene expression of Arabidopsis glutatione S-transferase, AtGST1 and AtGST11, in response to aluminum (Al) stress.2004

    • Author(s)
      B.Ezaki, M.Suzuki, H.Motoda, M.Kawamura, S.Nakashima, H.Matsumoto
    • Journal Title

      Plant Physiol. 134

      Pages: 1672-1682

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A wheat gene encoding an aluminum-activated malate transporter2004

    • Author(s)
      T.Sasaki, Y.Yamamoto, B.Ezaki, M.Katsuhara, S.J, Ahn, P.Ryan, E.Delhaize, H.Matsumoto
    • Journal Title

      Plant J. 37

      Pages: 645-653

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A novel cyanobacterial SmtB/ArsR family repressor regulates the expression of a CPx-ATPase and a metallothionein response to both Cu(I)/Ag(I) and Zn(II)/Cd(II).2004

    • Author(s)
      T.Liu, S.Nakashima, K.Hirose, M.shibasaka, M.Katsuhara, B.Ezaki, D.P.Giedroc, K.Kasamo
    • Journal Title

      J. Biol. Chem. 279

      Pages: 17810-17818

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A wheat gene encoding an aluminum-activated malate transporter.2004

    • Author(s)
      T.Sasaki, Y.Yamamoto, B.Ezaki, M.Katsuhara, S.J, Ahn, P.Ryan, E.Delhaize, H.Matsumoto
    • Journal Title

      Plant J. 37

      Pages: 645-653

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] A novel cyanobacterial SmtB/ArsR family repressor regulates the expression of a CPx-ATPase and a metallothionein in response to both Cu(I)/Ag(I) and Zn(II)/Cd(II).2004

    • Author(s)
      T.Liu, S.Nakashima, K.Hirose, M.Shibasaka, M.Katsuhara, B.Ezaki, D.P.Giedroc, K.Kasamo.
    • Journal Title

      J. Biol. Chem. 279

      Pages: 17810-17818

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mechanism of gene expression of Arabidopsis glutatione S-transferase, AtGST1 and AtGST11, in response to aluminum (Al) stress.2004

    • Author(s)
      B.Ezaki, M.Suzuki, H.Motoda, M.Kawamura, S.Nakashima, H.Matsumoto.
    • Journal Title

      Plant Physiol. 134

      Pages: 1672-1682

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A novel cyanobacterial SmtB/ArsR family repressor regulates the expression of a CPx-ATPase and a metallothionein in response to both Cu(I)/Ag(I) and Zn(II)/Cd(II).2004

    • Author(s)
      T.Lin, S.Nakashima, K.Hirose, M.Shibasaka, M.Katsuhara, B.Ezaki, D.P.Giedroc, K.Kasamo.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 17810-17818

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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