| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Research Abstract |
The objectives of this research are to 1) discover probiotic lactic acid bacteria as a functional food product enabling strong lipoperoxide breakdown, and 2) to accelerate its degrading capability being formed in the intestine.
In 2004, we developed an isolation medium separated off lactic acid bacteria that has lipoperoxide degradative enzyme. In addition, we isolated potential strains that possess strong degrading capability of peroxide. In 2005 identifying useful strains from isolated lactic acid bacteria, we evaluated degrading capability of peroxide-decomposition mechanism.
1. Standard Identification and Grouping : After basic identification and grouping of lactic acid bacteria (lactic acid bacteria Manual, Asakura Bookshop), we screened 48 strains potentially applicable for functional food product and excluded potential pathogenic strains from isolated strains. 4 out 48 strains performing high lipoperoxide decomposition were full-identified through 16sDNA Sequence and Taxonomy and
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Phenotypic Analysis. As a result of the identification, they are classified as a family of Lactobacillus plantarum.
2. Evaluation of Lipoperoxide Decomposition : For measuring degradation activity of lipoperoxide, we prepared for Lactobacillus Standard GYP medium in lack of lipid peroxide and divalent iron, and conditioned with aerobic culture at 37? and then harvested. We utilized linoleic acid as a lipid peroxide and cumene peroxide as a model compound to measure its activity. In addition, hydrogen peroxide was used as a hydrophilic substrate. Among isolated strains, Lactobacillus plantarum Pan1-2 performed the highest degrading activity of lipoperoxide. This unique strain fully degrades cumene peroxide, while showing approximately a half degrading activity for hydrogen peroxide as a hydrophilic substrate.
3. Peroxide Degradation Mechanism of Resting Cell : In presence of butyl peroxide for identifying reaction product of L.plantarum Pan1-2, the strain showed equal in reduction of butyl peroxide to butanol. As a result of that, it is suggested that the strain has 2-electron reduction of peroxide. Less
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