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Study on the evolution of Archaea and Bacteria from the view points of archaeal polar lipid biosynthesis

Research Project

Project/Area Number 16580067
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionUniversity of Occupational and Environmental Health, Japan

Principal Investigator

KOGA Yosuke  University of Occupational and Environmental Health, School of Medicine, Professor, 医学部, 教授 (70012458)

Co-Investigator(Kenkyū-buntansha) MORII Hiroyuki  University of Occupational and Environmental Health, School of Medicine, Associate Professor, 医学部, 助教授 (60141743)
TOH Hiroyuki  Kyushu University, Medical Institute of Bioregulation, Professor, 生体防御医学研究所, 教授 (70192656)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsArchaea / phospholipid / glycolipid / ether-type lipid / biosynthesis / phylogenetic tree / evolution pre-cell / pre・cell / イノシトールリン脂質 / 微生物 / テトラエーテル型脂質 / 酵素活性 / inositol-1-phosphate synthase / diglucosyl archaeol synthase / monoglucosyl archaeol synthase / 配列検索
Research Abstract

Polar lipids are indispensable constituents of cellular membranes. Because the first cell came into existence at the occurrence of polar lipids, the elucidation of differences in the structure and biosynthetic mechanism between Archaea and Bacteria would give a clue to discuss the origin of Archaea and Bacteria, which show differences in the fundamental biochemistry. Previous studies revealed that one of the most fundamenntal biochemical differences was the stereochemical structure of glycerophosphate backbone of membrane polar lipids, and it was proposed that the segregation of the chiral lipids triggered off differentiation of the Archaea and Bacteria. The present study focused on the evolution of polar groups of membrane polar lipids in relation with the differentiation of Archaea and Bacteria.
It was elucidated in the present study that synthases of inositol phospholipid and glycerol phospholipid belonged to the same enzyme family, CDP-alcohol phosphatidyltransferase family, along w … More ith serine phospholipid synthase including ester-type phospholipid synthases. This conclusion was drawn from BLAST search of archaeal and bacterial genomes using the amino acid sequence of archaetidylserine synthase as a query and in vitro studies of archaetidylserine synthase from a methanoarchaeon. Activities of monoglucosylarchaeol synthase and diglucosylarchaeol synthase were detected and partialy characterized. Saturated archaeol was sequentially glucosylated with UDP-glucose by two separate enzymes. Although glycolipid synthases in Archaea have not been phylogenetically elucidated, the outline of the enzymatic mode of reaction of archaeal glycolipid synthesis was the similar to bacterial counterparts. The archaeal monoglucosylarchaeol synthase recognized the stereostructure of the lipid substrate.
It was proposed from the above results that the polar lipids of pre-cells proposed by Wachtershauser at the stage before differentiation of Archaea and Bacteria had common and contemporary polar head groups shared by Archaea and Bacteria. Polar lipids with racemic core lipid and common polar groups were segregated into homochiral polar lipids carrying common polar groups. This was the cause of differentiation of Archaea and Bacteria. Homologous enzymes for phospholipid synthesis were carried over into both domains of organisms. Stereospecific glycolipid synthases would be developed after differentiation of Archaea and Bacteria. Less

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (22 results)

All 2007 2006 2005 2004

All Journal Article (22 results)

  • [Journal Article] Biosynthesis of ether-type polar lipids in Archaea and evolutionary considerations2007

    • Author(s)
      Y.Koga
    • Journal Title

      Microbiology and Molecular Biology Reviews 71・1

      Pages: 97-120

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Biosynthesis of ether-type polar lipids in Archaea and evolutionary considerations2007

    • Author(s)
      Koga, Y., Morii, H.
    • Journal Title

      Micobiology and Molecular Biology Reviews 71-1

      Pages: 97-120

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] biosynthesis of ether-type polar lipids in archaic and evolutionary considerations2007

    • Author(s)
      Y.Koga
    • Journal Title

      Microbiology and Molecular Biology Reviews 71・1

      Pages: 97-120

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Special methods for the analysis of ether lipid structure and metabolism in Archaea2006

    • Author(s)
      Y.Koga
    • Journal Title

      Analytical Biochemistry 348・1

      Pages: 1-14

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Identification of sn-glycerol-1-phosphate dehydrogenase activity from genomic information of a hypperthermophilic Archaea Sufolobus tokodaii 72006

    • Author(s)
      Y.Koga
    • Journal Title

      Bioscience, Biotechnology and Biochemistry 70・1

      Pages: 282-285

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Special methods for the analysis of ether lipid structure and metabolism in archaea2006

    • Author(s)
      Koga, Y., Morii, H.
    • Journal Title

      Analytical Biochemistry 348--1

      Pages: 1-14

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification of sn-glycerol-1-phosphate dehydrogenase activity from genomic information on a hyperthermophilic archaeon, Sulfolobus tokodaii strain 72006

    • Author(s)
      Koga, Y., Ohga, M., Tshujimura, M., Morii, H., Kawarabayasi, Y.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 70-1

      Pages: 282-285

    • NAID

      10018535824

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification of sn-glycerol-1-phosphate dehydrogenasc activity from genomic information of a hyperthermophilic archaic Sufolobus tokodaii strain 72006

    • Author(s)
      Y.Koga
    • Journal Title

      Bioscience, Biotechnology and Biochemistry 70・1

      Pages: 282-285

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Identification of sn-glycerol-1-phosphate dehydrogenase activity from genomic information of a hyperthermophilic archaeon Sulfolobus tokodaii strain 72006

    • Author(s)
      Koga, Y.
    • Journal Title

      Bioscience, Biotechnology and Biochemistry 70・1

      Pages: 282-285

    • NAID

      10018535824

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Special methods for the analysis of ether lipid structure and metabolism in Archaea2006

    • Author(s)
      Koga, Y.
    • Journal Title

      Analytical Biochemistry 348・1

      Pages: 1-14

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Recent advances in structural research on the ether lipids from Archaea, and its comparative and physiological aspects2005

    • Author(s)
      Y.Koga
    • Journal Title

      Bioscience, Biotechnology and Biochemistry 69・11

      Pages: 2019-2034

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A study on archaeal enzymes involved in polar lipids synthesis by an appproach linking the information about amino acid sequences, genomic contexts and lipid composition2005

    • Author(s)
      H.Daiyasu
    • Journal Title

      Archaea 1・6

      Pages: 399-410

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Recent advances in structural research on ether lipids from Archaea including comparative and physiological aspects2005

    • Author(s)
      Koga, Y.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 69-11

      Pages: 2019-2034

    • NAID

      130000030473

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A study of archaeal enzymes involved in polar lipid synthesis linking amino acid sequence information, genomic contexts and lipid composition2005

    • Author(s)
      Daiyasu, H., Kuma, K., Yokoi, T., Morii H., Koga, Y., Toh, H.
    • Journal Title

      Archaea 1-6

      Pages: 399-410

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Recent advances in structural research on the ether lipids from Archaea and its comparative and physiological aspects2005

    • Author(s)
      Koga, Y.
    • Journal Title

      Bioscience, Biotechnology and Biochemistry 69・11

      Pages: 2019-2034

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A study on archaeal enzymes involved in polar lipids synthesis by an approach linking the information about amino acid sequences, genomic contexts and lipid composition2005

    • Author(s)
      Daiyasu, H.
    • Journal Title

      Archaea 1・6

      Pages: 399-410

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Bacteria, Archaea, Eucaryaの分化-鏡像異性体脂質に基づく仮説-2004

    • Author(s)
      古賀 洋介
    • Journal Title

      生化学 76・4

      Pages: 349-358

    • NAID

      10012894782

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 古細菌新規エーテル型リン脂質に関する進化的・分類学的・生態学的研究2004

    • Author(s)
      古賀 洋介
    • Journal Title

      日本農芸化学会誌 78・7

      Pages: 622-629

    • NAID

      10013285987

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Diversification of Bacteria, Archaea, and Eucarya-A hypothesis based on the enantiomeric glycerophospholipids (in Japanese)2004

    • Author(s)
      Koga, Y.
    • Journal Title

      Seikagaku (Biochemistry in Japanese) 76-4

      Pages: 349-348

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The evolutionary, taxonomic, and ecological studies on novel ether-type lipids in archaea (in Japanese)2004

    • Author(s)
      Koga, Y.
    • Journal Title

      Nippon Nougeikagaku Kaishi 78-7

      Pages: 622-629

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Bacteria, Archaea, Eucaryaの分化--鏡像異性体脂質に基づく仮説--2004

    • Author(s)
      古賀 洋介
    • Journal Title

      生化学 76巻4号

      Pages: 349-358

    • NAID

      10012894782

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 古細菌新規エーテル型リン脂質に関する進化的・分類学的・生態学的研究2004

    • Author(s)
      古賀 洋介
    • Journal Title

      日本農芸化学会誌 78巻7号

      Pages: 622-629

    • NAID

      10013285987

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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