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Plant molecular breeding with the modified metabolic capacity of transitory starch for increase of biomass resources

Research Project

Project/Area Number 16580069
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

ITO Hiroyuki  Hokkaido Univ., Grad.School of Agr., Asso.Prof., 大学院・農学研究科, 助教授 (10241366)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsADP-glucose pyrophosphorylase / transitory starch / transgenic plants / mutated enzymes / allosteric regulation / 同化デンプン合成 / アロステリック調節 / starch-branching enzyme
Research Abstract

Plants have the ability to convert light energy to chemical energy and to fix atmospheric carbon dioxide by using the chemical energy. It is very important in the survival of plants to utilize the photoassimilate efficiently. It is generally known that the photosynthetic capacity is positively correlated with the accumulation of transitory starch. Therefore, to reinforce the metabolic capacity of transitory starch is expected to lead elevation of the photosynthetic capacity, the growth and the productivity. We have employed the modification of allosteric properties of ADP-glucose pyrophosphorylase (AGPase), which is the key enzyme of plant starch biosynthesis. Arabidopsis plants with modified allosteric AGPase showed the increase of metabolic capacity of transitory starch and thereby the plant growth. The aim in this study was to obtain the basic data for the improvement of rice plant productivity.
1.Expression of AGPase that is insensitive to known allosteric molecules
AGPase from Bacillus stearothermophillus is insensitive to known allosteric molecules. In this study, the gene for B.stearothermophillus AGPase (BsAGP) was introduced into Arabidopsis plants that is deficient in the leaf AGPase isoform. The AGPase activity and transitory starch levels in transgenic plants recovered only to the same levels as wild-type plants, indicating that BsAGP is regulated by unknown molecules in plants.
2.Organ-specific expression profiles of the genes for rice AGPase subunits
Analysis of RT-PCR products showed that 6 rice AGPase genes expressed differently in several organs. One of the 6 genes generated two transcripts by alteenative splicing, whose proteins located in different subcellular compartments, cytosol and amyloplasts.
3.Expression pattern of the candidate gene for rice ADP-glucose transporter
Analysis of RT-PCR products indicated that the transcripts for the candidate of rice ADP-glucose transporter accumulates predominantly in endosperms.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (17 results)

All 2006 2004

All Journal Article (17 results)

  • [Journal Article] Enhanced turnover of transitory starch by expression of up-regulated ADP-glucose pyrophosphorylases in Arabidopsis thaliana.2006

    • Author(s)
      Obana, Y., et al.
    • Journal Title

      Plant Science 170 (1)

      Pages: 1-11

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Enhanced turnover of transitory starch by expression of up-regulated ADP-glucose pyrophosphorylases in Arabidopsis thaliana.2006

    • Author(s)
      Obana, Y., et al.
    • Journal Title

      Plant Science 170(1)

      Pages: 1-11

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Enhanced turnover of transitory starch by expression of up-regulated ADP-glucose pyrophosphorylases in Arabidopsis thaliana.2006

    • Author(s)
      Y.Obana et al.
    • Journal Title

      Plant Science 170(1)

      Pages: 1-11

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Functional characteristics of C-terminal regions of starch-branching enzymes from developing seeds of kidney bean (Phaseolus vulgaris L.).2004

    • Author(s)
      Ito, H., et al.
    • Journal Title

      Plant Science 166 (5)

      Pages: 1149-1158

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Occurrence of multiple forms for starch synthase II isozyme in developing seeds of kidney bean.2004

    • Author(s)
      Isono, N., et al.
    • Journal Title

      Journal of Applied Glycoscience 51 (2)

      Pages: 101-107

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Ten rice peroxidases redundantly respond to multiple stresses including infection with rice blast fungus.2004

    • Author(s)
      Sasaki, K., et al.
    • Journal Title

      Plant and Cell physiology 45 (10)

      Pages: 1442-1452

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of starch synthase I and II expressed in early developing seeds of kidney bean (Phaseolus vulgaris L.).2004

    • Author(s)
      Senoura, T., et al.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 68 (9)

      Pages: 1949-1960

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Starch properties of the sago palm (Metroxylon sagu Rottb.) in different soils.2004

    • Author(s)
      Nozaki, K., et al.
    • Journal Title

      Plant Foods for Human Nutrition 59 (3)

      Pages: 85-92

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Functional characteristics of C-terminal regions of starch-branching enzymes from developing seeds of kidney bean (Phaseolus vulgaris L.).2004

    • Author(s)
      Ito, H., et al.
    • Journal Title

      Plant Science 166(5)

      Pages: 1149-1158

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Occurrence of multiple forms of starch synthase II isozyme in developing seeds of kidney bean.2004

    • Author(s)
      Isono, N., et al.
    • Journal Title

      Journal of Applied Glycoscience 51(2)

      Pages: 101-107

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Ten rice peroxidases redundantly respond to multiple stresses including infection with rice blast fungus.2004

    • Author(s)
      Sasaki, K., et al.
    • Journal Title

      Plant and Cell Physiology 45(10)

      Pages: 1442-1452

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of starch synthase I and II expressed in early developing seeds of kidney bean (Phaseolus vulgaris L.).2004

    • Author(s)
      Senoura, T., et al.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 68(9)

      Pages: 1949-1960

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Starch properties of the sago palm (Metroxylon sagu Rottb.) in different soils.2004

    • Author(s)
      Nozaki, K., et al.
    • Journal Title

      Plant Foods for Human Nutrition 59(3)

      Pages: 85-92

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Functional characteristics of C-terminal regions of starch-branching enzymes from developing seeds of kidney bean (Phaseolus vulgaris L.)2004

    • Author(s)
      H.Ito et al.
    • Journal Title

      Plant Science 166(5)

      Pages: 1149-1158

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Occurrence of multiple forms for starch synthase II isozyme in developing seeds kidney bean2004

    • Author(s)
      N.Isono et al.
    • Journal Title

      Journal Applied Glycoscience 51(2)

      Pages: 101-107

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Starch properties of the saga palm (Metroxylon sagu Rottb.) in different soils2004

    • Author(s)
      K.Nozaki et al.
    • Journal Title

      Plant Foods for Human Nutrition 59(3)

      Pages: 85-92

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Characterization of starch synthase I and II expressed in early developing seeds of kidney bean (Pheseolus vulgaris L.)2004

    • Author(s)
      T.Senoura et al.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 68(9)

      Pages: 1949-1960

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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