• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of inhibitor against intestinal bacterium infection by using artificial mucin

Research Project

Project/Area Number 16580072
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionShizuoka University

Principal Investigator

MURATA Takeomi  Shizuoka University, Faculty of Agriculture, Associate, 農学部, 助教授 (30273171)

Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsmucin / GlcNAc / glycosyltransferase / Helicobacter pylori / horse / serum / glycosyltransferase / transferase / Helycobctor pylori / poly glutamic acids
Research Abstract

Gastrointestinal mucins having GlcNAcα1,4Galβ-R are known to play protective roles against Helicobacter pylori infection. Our research is to develop a synthetic root of artificial mucin with a polyglutamic acid backbone carrying multivalent glycan, which functions as an inhibitor against H pylori infection.
1. Artificial. mucin
Mucins, highly glycosylated proteins that form the major component of mucosa, are known to play a physiological role as barrier to biomolecules. Highly water-soluble, artificial glycopolypeptides with a polyglutamic acid backbone carrying multivalent GlcNAcα1,4Galβ-units, termed 'artificial mucins' have been chemoenzymatically synthesized as potential polymeric inhibitors of infection by H. pylori. In the case, α1,4-N-acetylglucosaminyltransferase (α4GnT) in horse serum, which was obtained by screening of the enzyme activity in various kinds of serums, was used as a tool for synthesizing an epitope of GlcNAc□, 4Gal□-R glycoside.
2. Purification of α4GnT from horse serum
An α4GnT from horse serum was purified 5,300 fold by fractionation with ammonium sulfate, foolowed by sequential chromatographies by CM-Sepharose, Concanavalin A-agarose, Superdex 200 p.g, and UDP-hexanolamine agarose. The purified enzyme was highly specific for an acceptor substrate Galβ-3GalNAcαpNP having core 1 strucrure, on G1cNAc transfer from UDP-G1cNAc donor

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (26 results)

All 2007 2006 2005 2004

All Journal Article (24 results) Book (2 results)

  • [Journal Article] Chemoenzymatic synthesis of artificial glycopolypeptides containing multivalent sialyloligosaccharides with a γ-polyglutamic acid backbone and their effect for inhibition of infection by influenza2007

    • Author(s)
      M.Ogata, T.Murata, K.Murakami, T.Suzuki, KI Hidari, T.Usui
    • Journal Title

      Bioorg. Med. Chem. 15

      Pages: 1383-1393

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Chemoenzymatic synthesis of artificial glycopeptides containing multivalent sialyloligosaccharides with a γ-polygulutamic acid backbone and their effect on inhibition of infection by influenza2007

    • Author(s)
      Makoto Ogata
    • Journal Title

      Bioorg. Med. Chem. 15

      Pages: 1383-1393

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Crystal structure of the galectin-9 N-terminal carbohydrate recognition domain from Mus musculus reveals the basic mechanism of carbohydrate recognition2006

    • Author(s)
      M.Nagae, N.Nishi, T.Murata, T.Usui, T.Nakamura, S.Wakatuki, R.Kato
    • Journal Title

      J. Biol. Chem. 281

      Pages: 35884-35893

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Enzymatic synthesis of oligosaccharides and neoglycoconjugates2006

    • Author(s)
      T.Murata, T.Usui
    • Journal Title

      Biosci. Biotech. Biochem. 70

      Pages: 1049-1059

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Enzymatic synthesis of oligosaccharides and neoglyco-conjugates.2006

    • Author(s)
      T.Murata, T.Usui
    • Journal Title

      Biosci. Biotechnol. Biochem 70

      Pages: 1049-1059

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Crystal structure of the galectin-9 N-terminal carbohydrate recognition domain from Mus musculus reveals the basic mechanism2006

    • Author(s)
      Masamichi Nagae
    • Journal Title

      J. Biol. Chem. 281

      Pages: 35884-35893

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Enzymatic synthesis of oligosaccharides and neoglycoconjugates2006

    • Author(s)
      Takeomi Murata
    • Journal Title

      Biosci. Biotschnol. Biochem. 70

      Pages: 1049-1059

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Hemagglutinin mutations responsible for the binding of H5N1 influenza viruses to human-type receptor2006

    • Author(s)
      Shinya Yamada
    • Journal Title

      Nature 444

      Pages: 378-382

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Efficient synthesis of β-primeverosides as aroma precursors by transglycosylation of β-diglycosidase from Penicillium multicolor.2006

    • Author(s)
      Tsuruhami, K.
    • Journal Title

      J.Carbohydr.Chem 24

      Pages: 849-863

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Design and facile synthesis of neoglycolipids as lactosylceramide and transformation into glycoliposome2005

    • Author(s)
      Y.Harada, T.Murata, K.Totani, M.Yamazaki, T.Usui et al.
    • Journal Title

      Biosci. Biotech. Biochem. 66

      Pages: 801-807

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Enzymatic synthesis of poly-N-acetyllactosamines as potential substrates for endo-β-galactosidase-mediated hydrolytic and transglycosylation reactions2005

    • Author(s)
      T.Murata, H.Honda, T.Hattori, T.Usui
    • Journal Title

      Biochim. Biophys. Acta 1722

      Pages: 60-68

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Purification and characterization of a chitinase from Amycolatopsis orientalis with N-acetyllactosamine-repeating unit releasing activity2005

    • Author(s)
      T.Murata, S.Amarume, T.Hattori, S.Tokuyama, T.Usui et al.
    • Journal Title

      Biochim. Biophys. Res. Commun. 336

      Pages: 514-520

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Enzymatic synthesis of poly-N-acetyllactosamines as potential substrates for endo-β-galactosidasemediated hydrolytic and trans-glycosylation reactions2005

    • Author(s)
      T.Murata, H.Honda, T.Hattori, T.Usui
    • Journal Title

      Biochim. Biophys. Acta 1722

      Pages: 60-68

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Purification and characterization of a chitinase from Amycolatopsis orientalis with N-acetyllactosamine-repeating unit releasing activity.2005

    • Author(s)
      Murata, T
    • Journal Title

      Biochem.Biophys.Res.Commun. 336

      Pages: 2-2

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The effects of N-glycosylation sites and the N-terminal region on the biological function of β1,3-N-acetylglucosaminyltransferase 2 and its secretion.2005

    • Author(s)
      Kato, T.
    • Journal Title

      Biochem.Biophys.Res.Commun., 329

      Pages: 699-705

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Purification and characterization of a soluble recombinant human ST6Gal I functionally expressed in Escherichia coli.2005

    • Author(s)
      Hidari, I.-P.J.K.
    • Journal Title

      Glycoconj J., 22

      Pages: 1-11

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Enzymatic synthesis of poly-N-acetyllactosamines as potential substrates for endo-β-galactosidase-catalyzed hydrolytic and transglycosylation reactions2005

    • Author(s)
      T.Murata, H.Honda, T.Hattori, T.Usui
    • Journal Title

      Biochimica et Biophysics Acta 1722・1

      Pages: 60-68

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Dsign and facile synthesis of neoglycolipids as lactosylceramide mimetics and their transfoemation into glycoliposomes2005

    • Author(s)
      Y.Harada, T.Murata, K.Totani, T.Kajimoto, S.M.Masum, Y.Tamba, M.Yamazaki, T.Usui
    • Journal Title

      Biosci.Biotechnol.Biochem. 69・1

      Pages: 166-178

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Efficient production of human β-1, 3-N-acetylglucosaminyl-transferase-2 fused with green fluorescence protein in insect cell2004

    • Author(s)
      T.Kato, T.Murata, T.Usui, EY Park
    • Journal Title

      Biochem Engi. J. 19

      Pages: 15-23

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Efficient production of human β-1,3-N-acetylglucosamynyl-transferase-2 fused with green fluorescence protein in insect cell.2004

    • Author(s)
      Kato T., Murata T., Usui T., Park E-Y.
    • Journal Title

      Biochem. Engi. J. 19

      Pages: 15-23

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Transglycosylation and Condensation of Disaccharide Units Using Endo-type Glycosidases2004

    • Author(s)
      K.Totani, N.Yasutake, T.Murata, T.Usui
    • Journal Title

      Trends in Glycoscience and Glycotechnology 16

      Pages: 383-392

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Enhanced virulence of influenza A viruses with the haemagglutinin of the 1918 pandemic virus.2004

    • Author(s)
      D.Kobasa, A.Takada, K.Shinya, M.Hatta, P.Halfmann, S.Theriault, U.Suzuki, U.Nishimura, K.Mitamura, N.Sugaya, T.Usui, T.Murata, Y.Maeda, S.Watanabe, U.Suresh, T.Suzuki, Y.Suzuki, H.Peldmann, Y.Kawaokan.
    • Journal Title

      Nature 431

      Pages: 703-708

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Comparative analysis of GFPUV-B-1,3-N-acetylglucosaminyl- transferase 2 production in two insect-cell-based expression system.2004

    • Author(s)
      Kato, T., Murata, T., Usui, T., Park Enoch Y.
    • Journal Title

      Protein Expression and Purification 35

      Pages: 54-61

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Novel synthesis of lactosyl β-glycosides and analogs through cellulase-mediated transglycosylation of condensation reaction.2004

    • Author(s)
      Yasutake, N., Totani, K., Harada, Y., Haraguchi, S., Murata, T., Usui, T.
    • Journal Title

      J.Applied.Glycoscience 51

      Pages: 141-147

    • Related Report
      2004 Annual Research Report
  • [Book] 酵素法を組み合わせた生理活性オリゴ糖鎖の合成(分担執筆)「未来を拓く糖鎖科学」2005

    • Author(s)
      村田 健臣, 碓氷 泰市
    • Publisher
      金芳堂
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 「グリコシダーゼを中心とした重要オリゴ糖鎖単位の酵素合成」,糖鎖科学の最先端技術(分担)2005

    • Author(s)
      村田健臣
    • Total Pages
      10
    • Publisher
      シーエムシー出版
    • Related Report
      2005 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi