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Apoptosis-inducing activity of plant lectins and specific application to tumor cells

Research Project

Project/Area Number 16580091
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionUniversity of Shizuoka

Principal Investigator

ISEMURA Mamoru  University of Shizuoka, School of Food and Nutritional Sciences, Professor, 食品栄養科学部, 教授 (40028197)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordslectin / cytokine / anti-tumor / apoptosis / udo / kurokawa / wasabi / mistletoe / 免疫機能 / 癌細胞 / ブタ血漿 / インターロイキン / 遺伝子発現
Research Abstract

Lectins are proteins of non-immune origin with specific binding affinity for carbohydrate moiety of glycoconjugates. We have developed a new method to isolate lectins using affinity gels conjugated with porcine plasma proteins. In this study, we examined biological activities of lectins isolated by our method. Aralia cordate lectin (ACL) from udo showed activity to enhance gene and protein expression of proinflammatory cytokine IL-8 in Caco-2 cells. Our results also showed apoptosis inducing activity of Japanese mistletoe lectin, iris lectin, and muscari lectin in human leukemia U937 cells. In addition, we found that Boletopsis leucomelas lectin (BLL) from the edible mushroom kurokawa induced apoptosis in U937 cells by the mechanism incuding the G2/M cell cycle arrest in assocation with up-regulation of p21/Waf1 gene expression. The observed decrease in expression of bcl-2 was ascribable to the up-regulated expression of p21/Waf1 and correlated to the enhanced release of cytochrome c from mitochondria to cytoplasm, leading to the activation of caspase-9 in BLL-treated cells. These data suggest usefulness of our new method for lectin isolation and importance of examining biological activities of plant lectins in various animal systems.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (14 results)

All 2006 2005 2004

All Journal Article (14 results)

  • [Journal Article] Carbohydrate Specificity of Lectins from Boletopsis leucomelas and Aralia cordate.2006

    • Author(s)
      Y.Koyama
    • Journal Title

      Bioscience Biotechnology Biochemistry 70

      Pages: 542-545

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Carbohydrate Specificity of Lectins from Boletopsis leucomelas and Aralia cordate.2006

    • Author(s)
      Y.Koyama, T.Suzuki, S.Odani, S.Nakamura, J.Kominami, J.Hirabayashi, M.Isemura
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 70

      Pages: 542-545

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Carbohydrate Specificity of Lectins from Boletopsis leucomelas and Aralia cordate2006

    • Author(s)
      Y.Koyama
    • Journal Title

      Bioscience Biotechnology Biochemistry 70・2

      Pages: 542-545

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Stimulation of IL-8 production by Aralia cordate lectin in human colon carcinoma Caco-2 cells.2005

    • Author(s)
      Y.Koyama
    • Journal Title

      Bioscience Biotechnology Biochemistry 69

      Pages: 202-205

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Involvement of G2/M Cell Cycle Arrest and the Mitochondrial Pathway in Boletopsis leucomelaena (Pers.) Fayod (Agaricomycetideae) Lectin-Induced Apoptosis of Human Leukemia U937 Cells.2005

    • Author(s)
      Y.Koyama
    • Journal Title

      International Journal of Medicinal Mushrooms 7

      Pages: 201-212

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Stimulation of IL-8 production by Aralia cordate lectin in human colon carcinoma Caco-2 cells.2005

    • Author(s)
      Y.Koyama, T.Suzuki, A.Kajiya, M.Isemura
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 69

      Pages: 202-205

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Involvement of G2/M Cell Cycle Arrest and the Mitochondrial Pathway in Boletopsis leucomelaena (Pers.) Fayod (Agaricomycetideae) Lectin-Induced Apoptosis of Human Leukemia U937 Cells.2005

    • Author(s)
      Y.Koyama, T.Suzuki, A.Kajiya, M.Isemura
    • Journal Title

      International Journal of Medicinal Mushrooms 7

      Pages: 201-212

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Stimulation of IL-8 production by Aralia cordate lectin in human colon carcinoma Caco-2 cells2005

    • Author(s)
      Y.Koyama
    • Journal Title

      Bioscience Biotechnology Biochemistry 69・1

      Pages: 202-205

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Involvement of G2/M Cell Cycle Arrest and the Mitochondrial Pathway in Boletopsis leucomelaena (Pers.) Fayod (Agaricomycetideae) Lectin-Induced Apoptosis of Human Leukemia U937 Cells2005

    • Author(s)
      Y.Koyama
    • Journal Title

      International Journal of Medicinal Mushrooms 7・1-2

      Pages: 201-212

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Stimulation of IL-8 production by Aralia cordate lectin in human colon carcinoma Caco-2 cells.2005

    • Author(s)
      Y.Koyama
    • Journal Title

      Bioscience Biotechnology Biochemistry 69・1

      Pages: 202-205

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Simple purification of lectin -application to isolation of a Japanese mistletoe lectin.2004

    • Author(s)
      Y.Koyama
    • Journal Title

      Current Topics in Biotechnology 1

      Pages: 67-73

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Simple purification of lectin-application to isolation of a Japanese mistletoe lectin.2004

    • Author(s)
      Y.Koyama, T.Suzuki, A.Kajiya, Y.Matsushita, S.Odani, T.Kawakami, M.Sazuka, M.Isemura
    • Journal Title

      Current Topics in Biotechnology 1

      Pages: 67-73

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of green tea on gene expression of hepatic gluconeogenic enzymes in vivo.2004

    • Author(s)
      Y.Koyama
    • Journal Title

      Planta Medica 70・11

      Pages: 1100-1102

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Epigallocatechin-3-O-gallate inhibits fibroblast contraction of floating collagen gel : interaction between epigallocatechin-3-O-gallate and platelet derived growth factor.2004

    • Author(s)
      Y.Suzuki
    • Journal Title

      Bioscience Biotechnology Biochemistry 68・8

      Pages: 1817-1820

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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