| Project/Area Number |
16580092
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
|
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| Research Institution | KEIO UNIVERSITY |
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Principal Investigator |
SUGAI Takeshi Keio University, Faculty of Science and Technology, Associate Professor, 理工学部, 助教授 (60171120)
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| Project Period (FY) |
2004 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2004: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | microbial enzymes / yeast reduction / natural product synthesis / kinetic resolution / amino acids / 生物資源 / 環境受容型反応 |
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| Research Abstract |
Based on the chemo-enzymatic synthesis with complementary use of organic synthesis and enzyme-catalyzed transformation, the following researches were accomplished. 1) synthesis of heterocyclic amino acid containing proline, indoline-2-carboxylic acid, azetidine-2-carboxylic acid in enantiomerically pure forms. ; 2) synthesis of new building blocks for terpenoid synthesis, with a definite absolute stereochemistry in regard to quaternary carbon chiral center, by means of newly found yeast-catalyzed reduction of symmetrical cyclic diketones ; 3) nitrile hydratase and amidase originated from Rhodococcus rhodochrous-mediated transformation of prochiral nitriles into enantiomerically pure amide-carboxylate, and the transformation to α-methylamino acids.
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