| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Research Abstract |
1,Isolation of flower growth regulators from Streptomyces hygroscopicus
Organophosphorus compounds were found to be produced in the fermentation broths of Streptomyces hygroscopicus. They were isolated by column chromatography on silica gel and avicel. One organophosphorus compound showed inhibitory activity on the growth of Arabidopsis thaliana at an concentration of 100 mg/l.
2,Influence of glyphosate on flower morphogenesis and response of nitrogen metabolism to the application of glyphosate in Petunia hybrida.
The change of flower shape was observed in petunia corollas treated with 0.5 mM glyphosate. Glyphosate changed flower symmetry from actinomorphic type to zygomorphic type. The corollas treated with glyphosate increased free amino acid content, but free amino acid profiles of aromatic amino acids, soluble protein content and profiles in petunia corollas did not cause any significant changes. The contents of soluble phenolics, lignin, and IAA in the corollats were not significantl
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y affected by glyphosate treatment. In contrast, glyphosate reduced the RNA content of petunia corollas.
The results of nonprotein nitrogen metabolism in glyphosate-treated petunia corollas suggested that low concentration of glyphosate might effected on the regulation of the flower symmetry through the reduction of RNA biosynthesis.
3,Interaction between Aspterric Acid and Indole-3-acetic Acid on Reproductive Growth in Arabidopsis thaliana
Application of indole-3-acetic acid (IAA) with a pollen growth inhibitor, aspterric acid (AA), results in the recovery of the normal pollen development. In contrast, application of gibberellin (GA_3) with AA do not induce the normal pollen growth. In addition, application of different concentrations of IAA with AA reveal to shorten the period of growth from bolting to first flowering as compared to that treated with AA alone. Furthermore, stem length and number of flower bud treated with IAA and AA were similar to those of control. These results suggest that IAA may play an important role in reproductive growth of A. thaliana. Less
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