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Development of fluorescence-detected circular dichroism by modulated beam in the wavelength axial direction

Research Project

Project/Area Number 16580112
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Food science
Research InstitutionChubu University

Principal Investigator

YAMAMOTO Atsushi  Chubu University, Department of Food and Nutritional Sciences, Professor, 応用生物学部, 教授 (60360806)

Co-Investigator(Kenkyū-buntansha) KODAMA Shuji  Toyama Institute of Health, Department of Chemistry, Researcher, 化学部, 副主幹研究員 (70360807)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsFluorescence-detected circular dichroism / High-performance Liquid Chromatography / Fluorescence Spectrometer / Capillary electrophoresis / Borate complex / 波長軸変調型FDCD検出法 / フローセル / カテキン
Research Abstract

A novel technique is developed that permits fluorescence-detected circular dichroism (FDCD) detection in a conventional fluorescence spectrophotometer. The proposed detector consists of a polarizing prism and a retardation plate fitting on the excitation light side of the fluorescence spectrometer. But this technique was suffered from the appearance of an artifact wave on the excitation spectrum, which complicated the detection of the FDCD wave. To solve this problem, the scan speed of the spectrometer could not be helped slowing and the integration of the spectra by repetitive scans was required. It made us the stopped-flow measurement, so this technique was not suit for the real-time determination.
The enantiomeric purity determination of catechins in tea drinks was performed by the proposed system. To optimize the determination conditions, the enantiomeric purity of catechin (2-(3,4-dihydroxylphenyl)-3,4-dihydro-2H-1-benzopyran-3,5,7-triol) could be analyzed without pretreatment except for the filtration of tea sample.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (12 results)

All 2006 2005 2004

All Journal Article (12 results)

  • [Journal Article] Mechanism of enantioseparation of DL-pantothenic acid in ligand exchange capillary electrophoresis using diol-borate system2006

    • Author(s)
      S.Aizawa, A.Yamamoto, S.Kodama
    • Journal Title

      Electrophoresis 27

      Pages: 880-880

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Mechanism of enantioseparation of DL-pantothenic acid in ligand exchange capillary electrophoresis using diol-borate system2006

    • Author(s)
      Sen-ichi Aizawa, Atsushi Yamamoto, Shuji Kodama
    • Journal Title

      Electrophoresis 27

      Pages: 880-880

    • Related Report
      2005 Annual Research Report
  • [Journal Article] HPLC/fluorescence detected circular dichroism detection by three-dimensional fluorescence detector2005

    • Author(s)
      A.Yamamoto, S.Kodama, R.Ito, H.Nakazawa, H.Kumagai
    • Journal Title

      Annual Report of College of Bioscinece and Biotechnology 4

      Pages: 1-1

    • NAID

      110004628124

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Chiral ligand exchange micellar electrokinetic chromatography using borate anion as a central ion2005

    • Author(s)
      S.Kodama, A.Yamamoto, R.Iio, S.Aizawa, K.Nakagomi, K.Hayakawa
    • Journal Title

      Electrophoresis 26

      Pages: 3884-3884

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Chiral ligand exchange micellar electro-kinetic chromatography using borate anion as a central ion2005

    • Author(s)
      S.Kodama, A.Yamamoto, R.Iio, S.Aizawa, K.Nakagomi, K.Hayakawa
    • Journal Title

      Electrophoresis 26

      Pages: 3884-3884

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] HPLC/fluorescence detected circular dichroism detection by three-dimensional fluorescence detector2005

    • Author(s)
      Atsushi Yamamoto, Shuji Kodama, Rie Ito, Hiroyuki Nakazawa, Hiroki Kumagai
    • Journal Title

      Annual Report of College of Bioscinece and Biotechnology 4

      Pages: 1-1

    • NAID

      110004628124

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Chiral ligand, exchange micellar electrokinetic chromatography using borate anion as a central ion2005

    • Author(s)
      Shuji Kodama, Atsushi Yamamoto, Reiko Iio, Sen-ichi Aizawa, Kazuya Nakagomi, Kazuichi Hayakawa
    • Journal Title

      Electrophoresis 26

      Pages: 3884-3884

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Direct enantioseparation of catechin and epicatechin in tea drinks by 6-O-α-D-glucosyl-β-cyclodextrin-modified micellar electrokinetic chromatography2004

    • Author(s)
      S.Kodama, A.Yamamoto, A.Matsunaga, H.Yanai
    • Journal Title

      Electrophoresis 25

      Pages: 2892-2892

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Chiral ligand exchange capillary electrophoresis using borate anion sa a centralion2004

    • Author(s)
      S.Kodama, A.Yamamoto, R.Iio, K.Sakamoto, A.Matsunaga, K.Hayakawa
    • Journal Title

      Analyst 129

      Pages: 1238-1238

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Chiral ligand exchange capillary electrophoresis using borate anion as a central ion2004

    • Author(s)
      S.Kodama, A.Yamamoto, R.Iio, K.Sakamoto, A.Matsunaga, K.Hayakawa
    • Journal Title

      Analyst 129

      Pages: 1238-1238

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Direct enantioseparation of catechin and epicatechin in tea drinks by 6-O-α-D-glucosyl-β-cyclodextrin-modified micellar electrokinetic chromatography2004

    • Author(s)
      Shuji Kodama, Atsushi Yamamoto, Akinobu Matsunaga, Hiroko Yanai
    • Journal Title

      Electrophoresis 25

      Pages: 2892-2898

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Chiral ligand exchange capillary electrophoresis using borate anion as a central ion2004

    • Author(s)
      Shuji Kodama, Atsushi Yamamoto, Reiko Iio, Keiji Sakamoto, Akinobu Matsunaga, Kazuichi Hayakawa
    • Journal Title

      Analyst 129

      Pages: 1238-1242

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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