• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Application of a stress-responsive transcription factor to stress-tolerant transgenic trees

Research Project

Project/Area Number 16580126
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 林学・森林工学
Research InstitutionForestry and Forest Products Research Institute (FFPRI)

Principal Investigator

NANJO Tokihiko  FFPRI, Department of Molecular and Cell Biology, Senior researcher, 生物工学研究領域, 主任研究官 (50370284)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsenvironmental stress / ERF / AP2 domain transcription factor / expressed sequence tags / full-length cDNA / Populus / Expressed Sequence Tag(EST)
Research Abstract

ERF/AP2-domain proteins are a transcription factor specific to plants. These proteins are involved in floral development, ethylene-mediated signal transduction and environmental stress responses. The ERF/AP2 protein family includes dehydration-responsive element (DRE)-binding transcription factors (DREB1/CBF and DREB2), which regulate many stress-induced genes in Arabidopsis, rice and maize. cDNAs for a series of DREB1/CBF and DREB2 proteins were cloned as transfactors interacting with the DRE/CRT cis-acting element, which responds to cold or osmotic stress. To improve environmental stress tolerance in forest trees, we introduced Arabidopsis DREB1A gene into poplar (Populus nigra L.var. italica Koehne) by an Agrobacterium tumefaciens-mediated method. In total, 40 lines of transgenic poplar were constructed and further analyzed. None of these however showed stunted phenotypes or stress tolerance reported in Arabidopsis. As an Arabidopsis-derived transcription factor might not act expectedly in woody plants, we tried to find the homologous genes that belong to the ERF/AP2 protein family in poplar. Twenty five of genes encoding ERF/AP2 proteins were found in our poplar full-length cDNA collection and 11 of these were to belong to the DREB subfamily. Some clones encoding DREB proteins were found to be upregulated in response to stress treatments. These findings must be useful for understanding stress physiology and genetic modification in forest trees.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (17 results)

All 2006 2005 2004

All Journal Article (17 results)

  • [Journal Article] ポプラ完全長cDNAの大規模収集2006

    • Author(s)
      楠城時彦
    • Journal Title

      日本植物生理学会年会発表要旨集 47

      Pages: 325-325

    • NAID

      130006991859

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] A collection of poplar full-length cDNAs2006

    • Author(s)
      Tokihiko Nanjo
    • Journal Title

      Plant and Cell Physiology 47 (supplement)

    • NAID

      130006991859

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] ポプラ完全長cDNAデータベースの構築2006

    • Author(s)
      櫻井哲也
    • Journal Title

      日本植物生理学会年会発表要旨集 47

      Pages: 163-163

    • Related Report
      2005 Annual Research Report
  • [Journal Article] ポプラのDNA修復関連遺伝子の発現特性2006

    • Author(s)
      西口 満
    • Journal Title

      日本植物生理学会年会発表要旨集 47

      Pages: 155-155

    • Related Report
      2005 Annual Research Report
  • [Journal Article] ポプラにおけるERF/AP2ドメイン転写因子の解析と環境耐性樹木作出への応用2005

    • Author(s)
      楠城時彦
    • Journal Title

      日本森林学会大会学術講演集 116

    • NAID

      130004614893

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular analysis of ERF/AP2 domain transcription factor in poplar and its application to the development of stress-tolerant trees (Japanese)2005

    • Author(s)
      Tokihiko Nanjo
    • Journal Title

      Nihon Sinringakkai Taikai Gakujyutsu Kouenshu (Japanese) 116

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Profiles of subtracted cDNAs expressed during generation of norlignan in sugi (Cryptomeria japonica D.Don) sapwood.2005

    • Author(s)
      Yoshida, K.
    • Journal Title

      IAWPS2005 International Symposium on Wood Science and Technology 2

      Pages: 321-322

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The peroxidase multigene family in poplar ; Lignification, stress-induction, and organspecific expression.2005

    • Author(s)
      Tsutsumi, Y.
    • Journal Title

      IAWPS2005 International Symposium on Wood Science and Technology 1

      Pages: 56-57

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 組換え森林生物の環境安全性評価手法の開発に関する研究2005

    • Author(s)
      木下 勲
    • Journal Title

      農林水産省農林水産技術会議事務局研究成果 428

      Pages: 76-80

    • Related Report
      2005 Annual Research Report
  • [Journal Article] ポプラにおけるERF/AP2ドメイン転写因子の解析と環境耐性樹木作出への応用2005

    • Author(s)
      楠城時彦, 二村典宏, 西口満, 春日美江, 篠崎和子, 篠崎一雄, 篠原健司
    • Journal Title

      日本森林学会大会学術講演集 116

    • NAID

      130004614893

    • Related Report
      2004 Annual Research Report
  • [Journal Article] ストレス応答におけるポプラペルオキシダーゼ遺伝子の発現誘導および部位特異性2005

    • Author(s)
      堤祐司, 森永紗也佳, 楠城時彦, 篠原健司, 近藤隆一郎
    • Journal Title

      日本植物生理学会年会発表要旨集 46

      Pages: 133-133

    • NAID

      130006986639

    • Related Report
      2004 Annual Research Report
  • [Journal Article] ポプラペルオキシダーゼ遺伝子の単離とそれらのストレス誘導発現解析2005

    • Author(s)
      森永紗也佳, 佐々木慎弥, 楠城時彦, 篠原健司, 堤祐司, 近藤隆一郎
    • Journal Title

      リグニン討論会講演集 49

      Pages: 25-28

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Characterization of full-length enriched expressed sequence tags of stress-treated poplar leaves.2004

    • Author(s)
      Tokihiko Nanjo
    • Journal Title

      Plant and Cell Physiology 45(12)

      Pages: 1738-1748

    • NAID

      10014214221

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] ポストゲノム時代の樹木研究-モデル樹木ポプラをめぐる研究の現状と展望-2004

    • Author(s)
      楠城時彦
    • Journal Title

      林木の育種 211

      Pages: 35-37

    • NAID

      40006182900

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Characterization of full-length enriched expressed sequence tags of stress-treated poplar leaves2004

    • Author(s)
      Tokihiko Nanjo
    • Journal Title

      Plant and Cell Physiology 45

      Pages: 1738-1748

    • NAID

      10014214221

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular genetics of forest trees in a post-genome era (Japanese)2004

    • Author(s)
      Tokihiko Nanjo
    • Journal Title

      Rinboku no Ikushu (Japanese) 211

      Pages: 35-37

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of full-length enriched expressed sequence tags of stress-treated poplar leaves.2004

    • Author(s)
      Tokihiko Nanjo, Norihiro Futamura, Mitsuru Nishiguchi, Tomohiro Igasaki, Kazuo Shinozaki, Kenji Shinohara
    • Journal Title

      Plant and Cell Physiology 45(12)

      Pages: 1738-1748

    • NAID

      10014214221

    • Related Report
      2004 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi