Development of a novel method to conserve genetic information of fish by cryopreservation of primordial germ cells
| Project/Area Number |
16580149
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Tokyo University of Marine Science and Technology |
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Principal Investigator |
YOSHIZAKI Goro Tokyo University of Marine Science and Technology, Department of Marine Biosciences, Associate professor, 海洋科学部, 助教授 (70281003)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | cryopreservation / primordial germ cell / surrogate broodstock technology / germ cell transplantation / vasa / GFP / genetic resources / teleost / ニジマス / 多様性 / 遺伝子資源保存 / vasa遺伝子 / 非翻訳領域 |
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| Research Abstract |
A method for cryopreserving fish embryos and eggs has previously been lacking. In this study, we have regenerated live offspring from cryopreserved materials for the first time in lower vertebrates. Our novel method allows the successful cryopreservation of rainbow trout primordial germ cells (PGCs). Moreover, through transplantation into allogenic recipients, the cryopreserved PGCs can develop into functional eggs and produce viable fry upon fertilization with cryopreserved spermatozoa. PGC cryopreservation and transplantation will allow the conservation of genetic information from endangered fish species. Our system will also be a powerful tool for maintaining domesticated fish strains carrying commercially valuable traits.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] GFP-labeling of primordial germ cells using a non-transgenic method and its application for germ cell transplantation in salmonidae.2005
Author(s)
Yoshizaki, G., Tago, Y., Takeuchi, Y., Sawatari, E., Kobayashi, T., Takeuchi, T.
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Journal Title
Biology of Reproduction 73
Pages: 88-93
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] GFP-labeling of primordial germ cells using a non-transgenic method and its application for germ cell transplantation in salmonidae.2005
Author(s)
Yoshizaki, G., Tago, Y., Takeuchi, Y., Sawatari, E., Kobayashi, T., Takeuchi, T.
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Journal Title
Biology of Reproduction, 73
Pages: 88-93
Description
「研究成果報告書概要(欧文)」より
Related Report
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