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Development of a novel method to conserve genetic information of fish by cryopreservation of primordial germ cells

Research Project

Project/Area Number 16580149
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionTokyo University of Marine Science and Technology

Principal Investigator

YOSHIZAKI Goro  Tokyo University of Marine Science and Technology, Department of Marine Biosciences, Associate professor, 海洋科学部, 助教授 (70281003)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordscryopreservation / primordial germ cell / surrogate broodstock technology / germ cell transplantation / vasa / GFP / genetic resources / teleost / ニジマス / 多様性 / 遺伝子資源保存 / vasa遺伝子 / 非翻訳領域
Research Abstract

A method for cryopreserving fish embryos and eggs has previously been lacking. In this study, we have regenerated live offspring from cryopreserved materials for the first time in lower vertebrates. Our novel method allows the successful cryopreservation of rainbow trout primordial germ cells (PGCs). Moreover, through transplantation into allogenic recipients, the cryopreserved PGCs can develop into functional eggs and produce viable fry upon fertilization with cryopreserved spermatozoa. PGC cryopreservation and transplantation will allow the conservation of genetic information from endangered fish species. Our system will also be a powerful tool for maintaining domesticated fish strains carrying commercially valuable traits.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (11 results)

All 2006 2005 2004 Other

All Journal Article (9 results) Book (2 results)

  • [Journal Article] Testicular germ cells can colonize sexually undifferentiated embryonic gonad and produce functional eggs in fish.2006

    • Author(s)
      Okutsu T, Suzuki K, Takeuchi Y, Takeuchi T, Yoshizaki G.
    • Journal Title

      Proc Natl Acad Sci USA. 103

      Pages: 2725-2729

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Testicular germ cells can colonize sexually undifferentiated embryonic gonad and produce functional eggs in fish.2006

    • Author(s)
      Okutsu T, Suzuki K, Takeuchi Y, Takeuchi T, Yoshizaki G
    • Journal Title

      Proc Natl Acad Sci USA. 103

      Pages: 2725-2729

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] GFP-labeling of primordial germ cells using a non-transgenic method and its application for germ cell transplantation in salmonidae.2005

    • Author(s)
      Yoshizaki, G., Tago, Y., Takeuchi, Y., Sawatari, E., Kobayashi, T., Takeuchi, T.
    • Journal Title

      Biology of Reproduction 73

      Pages: 88-93

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] GFP-labeling of primordial germ cells using a non-transgenic method and its application for germ cell transplantation in salmonidae.2005

    • Author(s)
      Yoshizaki, G., Tago, Y., Takeuchi, Y., Sawatari, E., Kobayashi, T., Takeuchi, T.
    • Journal Title

      Biology of Reproduction, 73

      Pages: 88-93

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] GFP labeling of primordial germ cells using a non-transgenic method and its application for germ cell transplantation in salmonidae.2005

    • Author(s)
      Yoshizaki G
    • Journal Title

      Biology of Reproduction (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Surrogate brood stock produces salmonids.2004

    • Author(s)
      Takeuchi, Y., Yoshizaki, G., Takeuchi, T.
    • Journal Title

      Nature 430

      Pages: 629-630

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Surrogate broodstock produces salmonids.2004

    • Author(s)
      Takeuchi, Y., Yoshizaki, G., Takeuchi, T.
    • Journal Title

      Nature 430

      Pages: 629-230

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Generation of viable fish from cryopreserved primordial germ cells.

    • Author(s)
      Kobayashi T, Takeuchi Y, Takeuchi T, Yoshizaki G.
    • Journal Title

      Molecular Reproduction and Development (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Generation of viable fish from cryopreserved primordial germ cells

    • Author(s)
      Kobayashi T, Takeuchi Y, Takeuchi T, Yoshizaki G.
    • Journal Title

      Molecular Reproduction and Development (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] サケからマスをつくる"始原生殖細胞を用いた魚類の発生工学"、クローンとエピジェネティクスの新展開(今川和彦 編)2006

    • Author(s)
      吉崎悟郎
    • Total Pages
      21
    • Publisher
      アドスリー
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] いざ生の扉へ・クローンとエピジェネティクスの新展開 7章 サケからマスをつくる・始原生殖細胞を用いた魚類の発生工学2006

    • Author(s)
      吉崎悟朗
    • Total Pages
      23
    • Publisher
      アドスリー
    • Related Report
      2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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