• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Photoactive hemolytic toxin in Chattonella marina

Research Project

Project/Area Number 16580154
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionNagasaki University

Principal Investigator

ODA Tatsuya  Nagasaki University, Faculty of Fisheries, Professor, 水産学部, 教授 (60145307)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Kenichi  Nagasaki University, Faculty of Fisheries, Associate Professor, 水産学部, 助教授 (90363473)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsRed tide / Chattonella / Hemolytic toxin / Photoactive / Cytotoxicity / 活性酵素 / 溶血活性物質 / 光活性化
Research Abstract

Chattonella marina (C.marina), a raphidophycean flagellate, is a causative organism of red tide, and highly toxic to fish. In this study, we found that the cell-free methanol extract prepared from this flagellate exhibited potent hemolytic activity against rabbit erythrocytes. Interestingly, the hemolytic activity of the extract was absolutely light-dependent, and no hemolytic activity was detected in the dark even at very high concentration. Gel filtration chromatography of the methanol extract on a column of Sephadex LH-20 revealed that the extract contained hemagglutinin as well as hemolytic agents, and the substances responsible for these activities were separately eluted. These results suggest that the hemagglutinating and hemolytic activities were derived from distinct compounds. The hemolytic fraction obtained after gel filtration (F4) caused marked inhibition of the growth of C.marina itself and other species of phytoplanktons. Furthermore, F4 showed a potent cytotoxicity toward various mammalian cultured cell lines including human tumor cells (HeLa cells) in a dose-dependent manner. The cytotoxicity was also light-dependent, and no cytotoxic effect was exhibited in any cell lines tested in the dark. After further purification procedures via preparative thin-layer chromatography and subsequent HPLC, a major hemolytic agent was obtained as highly purified form. Since the methanol extracts prepared from other raphidophycean flagellates such as Heterosigma akashiwo, Olisthodiscus luteus, and Fibrocapsa japonica showed light-dependent hemolytic activity toward rabbit erythrocytes, it was suggested that the light-dependent hemolytic agents commonly exist at least in these raphidophycean flagellates.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (25 results)

All 2005 2004

All Journal Article (25 results)

  • [Journal Article] Cytotoxicity of GalNAc-specific C-type lectin on various cell lines.2005

    • Author(s)
      T.Kuramoto, H.Uzuyama, T.Hatakeyama, T.Tamura, T.Nakashima, K.Yamaguchi, T.Oda
    • Journal Title

      J.Biochem. 137・1

      Pages: 41-50

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Purification and characterization of photosensitizing hemolytic toxin from harmful red tide phytoplankton,Heterocapsa circularisquama.2005

    • Author(s)
      Y.Miyazaki, T.Nakashima, T.Iwashita, T.Fujita, K.Yamaguchi, T.Oda
    • Journal Title

      Aquatic Toxicology 73・4

      Pages: 382-393

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Isolation and characterization of light-dependent hemolytic cytotoxin from harmful red tide phytoplankton Chattonella marina..2005

    • Author(s)
      A.Kuroda, T.Nakashima, K.Yamaguchi, T.Oda
    • Journal Title

      Comparative Biochemistry and Physiology,Part C 141

      Pages: 297-305

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cytotoxicity of GalNAc-specific C-type lectin on various cell lines.2005

    • Author(s)
      T.Kuramoto, H.Uzuyama, T.Hatakeyama, T.Tamura, T.Nakashima, K.Yamaguchi, T.Oda
    • Journal Title

      J.Biochem., 137-1

      Pages: 41-50

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Purification and characterization of photosensitizing hemolytic toxin from harmful red tide phytoplankton, Heterocapsa circularisquama.2005

    • Author(s)
      Y.Miyazaki, T.Nakashima, T.Iwashita, T.Fujita, K.Yamaguchi, T.Oda
    • Journal Title

      Aquatic Toxicology, 73

      Pages: 382-393

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Isolation and characterization of light-dependent hemolytic cytotoxin from harmful red tide phytoplankton Chattonella marina.2005

    • Author(s)
      A.Kuroda, T.Nakashima, K.Yamaguchi, T.Oda
    • Journal Title

      Comparative Biochemistry and Physiology, Part C, 141

      Pages: 297-305

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cytotoxicity of GaINAc-specific C-type lectin on various cell lines.2005

    • Author(s)
      T.Kuramoto, H.Uzuyama, T.Hatakeyama, T.Tamura, T.Nakashima, K.Yamaguchi, T.Oda
    • Journal Title

      J. Biochem. 137・1

      Pages: 41-50

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Purification and characterization of photosensitizing hemolytic toxin from harmful red tide phytoplankton, Heterocapsa circularisquama.2005

    • Author(s)
      Y.Miyazaki, T.Nakashima, T.Iwashita, T.Fujita, K.Yamaguchi, T.Oda
    • Journal Title

      Aquatic Toxicology 73・4

      Pages: 382-393

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Isolation and characterization of light-dependent hemolytic cytotoxin from harmful red tide phytoplankton Chattonella marina..2005

    • Author(s)
      A.Kuroda, T.Nakashima, K.Yamaguchi, T.Oda
    • Journal Title

      Comparative Biochemistry and Physiology, Part C 141

      Pages: 297-305

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Deleterious effect of Chattonella marina on short-necked clam(Ruditapes philippinarum);possible involvement of reactive oxygen species.2004

    • Author(s)
      D.Kim, O.Kumamoto, K.-S.Lee, A.Kuroda, A.Fujii, A.Ishimatsu, T.Oda
    • Journal Title

      J.Plankton Res. 26・8

      Pages: 967-971

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Production of superoxide anion and hydrogen peroxide by the red tide dinoflagellate Karenia mikimotoi2004

    • Author(s)
      Y.Yamasaki, D-I.Kim, Y.Matsuyama, T.Oda, T.Honjo
    • Journal Title

      J.Biosci.Bioeng. 97・3

      Pages: 212-215

    • NAID

      110002665286

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of the recombinant CEL-I,a GalNAc-specific C-type lectin,expressed in Escherichia coli using an arfificial synthetic gene.2004

    • Author(s)
      T.Hatakeyama, K.Shiba, N.Matsuo, T.Fujimoto, T.Oda, H.Sugawara, H.Aoyagi
    • Journal Title

      J.Biochem 135・1

      Pages: 101-107

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Mitogenic properties of pokweed lectin-D isoforms on human peripheral blood lymphocytes : non-mitogen PL-D1 and mitogen PL-D2.2004

    • Author(s)
      K.Yamaguchi, M.Uechi, Y.Katakura, T.Oda, M.Ishiguro
    • Journal Title

      Biosci.Biochechnol.Biochem. 68・7

      Pages: 1591-1593

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Deleterious effect of Chattonella marina on short-necked clam (Ruditapes philippinarum) ; possible involvement of reactive oxygen species.2004

    • Author(s)
      D.Kim, O.Kumamoto, K.-S.Lee, A.Kuroda, A.Fujii, A.Ishimatsu, T.Oda
    • Journal Title

      J.Plankton Res., 26-8

      Pages: 967-971

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Production of superoxide anion and hydrogen peroxide by the red tide dinoflagellate Karenia mikimotoi2004

    • Author(s)
      Y.Yamasaki, D-I.Kim, Y.Matsuyama, T.Oda, T.Honjo
    • Journal Title

      J.Biosci.Bioeng., 97-3

      Pages: 212-215

    • NAID

      110002665286

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of the recombinant CEL-I, a GalNAc-specific C-type lectin, expressed in Escherichia coli using an artificial synthetic gene.2004

    • Author(s)
      T.Hatakeyama, K.Shiba, N.Matsuo, T.Fujimoto, T.Oda, H.Sugawara, H.Aoyagi
    • Journal Title

      J.Biochem., 135-1

      Pages: 101-107

    • NAID

      10016197654

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Mitogenic properties of pokweed lectin-D isoforms on human peripheral blood lymphocytes : non-mitogen PL-D1 and mitogen PL-D2.2004

    • Author(s)
      K.Yamaguchi, M.Uechi, Y.Katakura, T.Oda, M.Ishiguro
    • Journal Title

      Biosci.Biochechnol.Biochem., 68-7

      Pages: 1591-1593

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of the recombinant CEL-I, a GaINAc-specific C-type lection, expressed in Escherichia coli using an artificial synthetic gene.2004

    • Author(s)
      T.Hatakeyama, K.Shiba, N.Matsuo, T.Fujimoto, T.Oda, H.Sugawara, H.Aoyagi
    • Journal Title

      J. Biochem. 135

      Pages: 101-107

    • Related Report
      2004 Annual Research Report
  • [Journal Article] In vivo evidence that peptide vaccination can induce HLA-DR-restricted CD4+ T cells reactive to a class I tumor peptide.2004

    • Author(s)
      M.Harada, R.Gohara, S.Matsueda, A.Muto, T.Oda, Y.Iwamoto, K.Itoh
    • Journal Title

      J. Immunol. 172

      Pages: 2659-2667

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Induction of cytokines by toxins that have an identical RNA N-glycosidase activity : Shiga Toxin, ricin, and modeccin.2004

    • Author(s)
      C.Yamasaki, K.Nishikawa, X, -T, Zeng, Y.Katayama, Y.Natori, N.Komatsu, T.Oda, Y.Natori
    • Journal Title

      Biochem.Biophysi.Acta 1671

      Pages: 44-50

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Production of superoxide anion hydrogen peroxide by the red tide dinoflagellate Karenia mikimotoi.2004

    • Author(s)
      Y.yamasaki, D-I.Kim, Y.Matsuyama, T.Oda, T.Honjo
    • Journal Title

      J.Biosci.Bioeng. 97

      Pages: 212-215

    • NAID

      110002665286

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Deleterious effect of Chattonella marina on short-necked clam(Ruditapes philillonarum);possible involvement of reactive oxygen species.2004

    • Author(s)
      D.Kim, O.Kumamoto, K.-S.Lee, A.Kuroda, A.Fujii, A.Ishimatsu, T.Oda
    • Journal Title

      J.Plankton Res. 26

      Pages: 967-971

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Role of p38 MAP Kinase Pathway in a Toxin-induced Model of Hemolytic Uremic Syndrome.2004

    • Author(s)
      X.J.Fu, K.Iijima, K.Nozu, K.Hamahira, R.Tanaka, T Oda, N.Yoshikawa, M.Mtsuo
    • Journal Title

      Pediatric Nephrology 19

      Pages: 844-852

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Direct evidence for the reduction of water flow across the gills of Jack mackerel (Trachurys japonicus) by a harmful alga Chattonella marina.2004

    • Author(s)
      K.-S.Lee, A.Ishimatsu, T.Oda
    • Journal Title

      J.Fisheies Science and Technology 7

      Pages: 96-98

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Mitogenic properties of pokweed lectin-D isoforms on human peripheral blood lymphocytes : non-mitogen PL-D1 and mitogen PL-D22004

    • Author(s)
      K.Yamaguchi, M.Uechi, Y.Katakura, T.Oda, M.Ishiguro
    • Journal Title

      Biosci.Biochechnol.Biochem. 68

      Pages: 1591-1593

    • Related Report
      2004 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi