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Studies on osmoregulation by neurosecretory system in crustaceans

Research Project

Project/Area Number 16580171
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionSuntory Institute for Bioorganic Research

Principal Investigator

YASUDA Akikazu  Suntory Institute for Bioorganic Research, Researcher, 主席研究員 (90211614)

Co-Investigator(Kenkyū-buntansha) YASUDA Yoshimi (KAMATANI Yoshimi)  Suntory Institute for Bioorganic Research, Researcher, 研究員 (10270571)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordscrayfish / neuropeptide / osmoregulation / anal respiration / orcokinin / tachykinin-related peptide / crustacean cardioactive peptide / crustacean-SIFamide / マイオサプレッシン / SIFアミド / 腹部神経節 / CCAP / MALDI-TOF MS / 甲殻類心臓作用性ペプチド / アラトスタチン関連ペプチド / FLRFアミド関連ペプチド
Research Abstract

To investigate osmoregulation by the central nerve system in crustaceans, topological mass spectrometry analysis was available for the identification of neuropeptides. In the crayfish (Procambarus clarkii), "anal respiration" has been described in the book "The Crayfish : An Introduction to the Study of Zoology" published in 1880. The observation has been noted that water is alternately taken into and expelled from the anus fifteen to seventeen times in a minute and it seemed to be regulated by the sixth abdominal ganglion. Direct matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry analysis with slice of the sixth abdominal ganglion was resulted in the presence of orcokinin and its gene-related peptides, crustacean-SIFamide, decapod tachykinin-related peptide, crustacean cardioactive peptide, myosupressin-like peptide. These peptides were examined in vivo on bioassays for rhythmic anal contraction and anal water flux. The activity of the peptides on the is … More olated intestine was also examined in vitro. Rhythmic anal contraction was caused by orcokinins, decapod tachykinin-related peptide, when each sample of 100 pmol was injected into the crayfish. Crustacean cardioactive peptide had most potent activity of anal opening. Water flux through the anus was observed by injection of crustacean cardioactive peptide or decapod tachykinin-related peptide. Interestingly, the action of the neuropeptides on the isolated intestine was diversified. The contraction of hindgut was activated by orcokinins, but not by crustacean cardioactive peptide or myosupressin-like peptide. Decapod tachykinin-related peptide showed repetitive action alternating between contraction and relaxation on the rectum. Crustacean-SIFamide caused complex movements of the hindgut, and induced most potent activity of defecation. We concluded that the crayfish can take water from the anus, but each function of neuropeptides identified in sixth abdominal ganglion remains unclear. We also think that "anal respiration" might not be a normal phenomenon, since the action was able to be reproduced by the overdose of a combination with crustacean-SIFamide and decapod tachykinin-related peptide. Less

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (19 results)

All 2006 2005 2004

All Journal Article (18 results) Book (1 results)

  • [Journal Article] Characteristic expression patterns of allatostatin-like peptide, FMRFamide-related peptide, orcokinin, tachykinin-related peptide, and SIFamide in the olfactory system of crayfish Procambarus clarkii.2006

    • Author(s)
      Yasuda-Kamatani, Y., Yasuda, A
    • Journal Title

      J. Comp. Neurol. 496

      Pages: 135-147

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Posttranslational processing of proopiomelanocortin family molecules in sea lamprey based on mass spectrometric and chemical analyses.2006

    • Author(s)
      Takahashi, A. et al.
    • Journal Title

      Gen Comp Endocrinol. 148

      Pages: 79-84

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Posttranslational processing of proopiomelanocortin family molecules in sea lamprey based on mass spectrometric and chemical analyses.2006

    • Author(s)
      Takahashi, A., Yasuda, A., Sower, S., Kawauchi, H.
    • Journal Title

      Gen Comp Endocrinol. 148

      Pages: 79-84

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nucleotide sequence and expression of three subtypes of proopiomelanocortin mRNA in barfin flounder.2005

    • Author(s)
      Takahashi, A et al.
    • Journal Title

      Gen. Comp. Endocrinol. 141

      Pages: 291-303

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Characteristic expression patterns of allatostatin-like peptide, FMRFamide-related peptide, orcokinin, tachykinin-related peptide, and SIFamide in the olfactory system of crayfish Procambarus clarkii.2005

    • Author(s)
      Yasuda-Kamatani, Y., Yasuda, A.
    • Journal Title

      J.Comp.Neurol. 496

      Pages: 135-147

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nucleotide sequence and expression of three subtypes of proopiomelanocortin mRNA in barfin flounder2005

    • Author(s)
      Takahashi, A., Amano, M., Itoh, T., Yasuda, A., Yamanome, T., Amemiya, Y., Sasaki, K., Sakai, M., Yamamori, K., Kawauchi, H
    • Journal Title

      Gen.Comp.Endocrinol. 141

      Pages: 291-303

    • Related Report
      2005 Annual Research Report
  • [Journal Article] APSGFLGMRamide is a unique tachykinin-related peptide in crustaceans.2004

    • Author(s)
      Yasuda-Kamatani, Y., Yasuda, A
    • Journal Title

      Eur. J. Biochem. 271

      Pages: 1546-1556

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Prepro-tachykinin gene expression in the brain of the honeybee Asis mellifera.2004

    • Author(s)
      Takeuchi, H et al.
    • Journal Title

      Cell Tissue Res. 316

      Pages: 281-293

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Possible involvement of melanin-concentrating hormone in food intake in a teleost fish, barfin flounder.2004

    • Author(s)
      Takahashi, A. et al.
    • Journal Title

      peptides 25

      Pages: 1613-1622

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] APSGFLGMRamide is a unique tachykinin-related peptide in crustaceans.2004

    • Author(s)
      Yasuda-Kamatani, Y., Yasuda, A.
    • Journal Title

      Eur.J.Biochem. 271

      Pages: 1546-1556

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Prepro-tachykinin gene expression in the brain of the honeybee Asis mellifera.2004

    • Author(s)
      Takeuchi, H., Yasuda, A., Yasuda-Kamatani, Y., Sawata, M., Mastuo, Y., Kata, A., Tsujimoto, A., Nakajima, T., Kubo, T.
    • Journal Title

      Cell Tissue Res. 316

      Pages: 281-293

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Possible involvement of melanin-concentrating hormone in food intake in a teleost fish, barfin flounder.2004

    • Author(s)
      Takahashi, A., Tsuchiya, K., Yamanome, T., Amano, M., Yasuda, A., Yamamori, K., Kawauchi, H.
    • Journal Title

      Peptides 25

      Pages: 1613-1622

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nucleotide sequence and expression of three subtypes of proopiomelanocortin mRNA in barfin flounder.2004

    • Author(s)
      Takahashi, A., Amano, M., Itoh, T., Yasuda, A., Yamanome, T., Amemiya, Y., Sasaki, K., Sakai, M., Yamamori, K., Kawauchi, H.
    • Journal Title

      Gen.Comp.Endocrinol. 141

      Pages: 291-303

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] New strategy for the exploration of neuropeptides (in Japanese)2004

    • Author(s)
      Yasuda, A., Yasuda, Y.
    • Journal Title

      Kagaku to Seibutu 42

      Pages: 834-841

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] APSGFLGMRamide is a unique tachykinin-related peptide in crustaceans2004

    • Author(s)
      Yasuda-Kamatani, Y., Yasuda, A.
    • Journal Title

      Eur.J.Biochem. 271

      Pages: 1546-1556

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Prepro-tachykinin gene expression in the brain of the honeybee Apis mellifera2004

    • Author(s)
      Takeuchi, H., Yasuda, A., Yasuda-Kamatani, Y., Sawata, M., Matsuo, Y.
    • Journal Title

      Cell Tissue Res. 316

      Pages: 281-293

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Possible involvement of melanin-concentratuing hormone in food intake in a teleost fish, barfin flounder2004

    • Author(s)
      Takahashi, A., Tsuchiya, K., Yamanome, T., Amano, M., Yasuda, A., Yamamori, K., Kawauchi, H.
    • Journal Title

      Peptides 25

      Pages: 1613-1622

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 新規生理活性ペプチドの新しい探索法2004

    • Author(s)
      安田明和, 安田好美
    • Journal Title

      化学と生物 42

      Pages: 834-841

    • NAID

      10016520502

    • Related Report
      2004 Annual Research Report
  • [Book] 化学と生物2004

    • Author(s)
      安田明和, 安田好美
    • Publisher
      新規生理活性ペプチドの新しい探索法
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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