| Project/Area Number |
16580227
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Shinshu University |
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Principal Investigator |
ONO Tamao SHINSHU UNIVERSITY, faculty of Agriculture, Professor, 農学部, 教授 (10177264)
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| Co-Investigator(Kenkyū-buntansha) |
KAGAMI Hiroshi SHINSHU UNIVERSITY, Faculty of Agriculture, Professor, 農学部, 教授 (80308303)
ROH Sanggun SHINSHU UNIVERSITY, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (90322130)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | chicken / quail / blue-breasted quail / embryo genesis / primordial germ cells / chimera / PCR / antibody / in situハイブリダイゼーション |
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| Research Abstract |
The aim of this project was the production and reserve of endangered, rare and valuable birds using the technique of germ cell transfer. We developed the protocol to obtain a large amount of PGCs from gonads of chicken and quail embryos using PGC specific monoclonal antibodies (anti-SSEA1, 2C9 and QCR1) and magnetism activated cell sorting (MACS). MACS treatment greatly increased the population ratio of avian PGCs in gonadal cells, resulting improved gonadal migration in recipient embryos. Blastodermal and primordial germ cells were transferred to the recipient avian embryos. Treated chimeras were hatched and the donor-derived cells were tracked using species-specific DNA markers. Donor derived genome was observed using PCR and Immunohistochemistry in chimeric quail which introduced blastodermal cells of chicken and blue-breasted quail embryos.
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