• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Ghrelin peptides induced contractions and growth hormone secretagogue receptor expression in the chicken gastrointestinal tract

Research Project

Project/Area Number 16580245
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionRakuno Gakuen University

Principal Investigator

KITAZAWA Takio  Rakuno Gakuen University, School of Veterinary Medicine, Professor, 獣医学部, 教授 (50146338)

Co-Investigator(Kenkyū-buntansha) ONAGA Takenori  Rakuno Gakuen University, School of Veterinary Medicine, Professor, 獣医学部, 助教授 (90224261)
IWANO Hidetomc  Rakuno Gakuen University, School of Veterinary Medicine, Professor, 獣医学部, 助教授 (60382488)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2006: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsGhrelin / Chicken gastrointestinal tract / Growth hormone secretagogue / Ghrelin receptor / Smooth muscle / Enteric nerve / Mucosa / ニワトリ / 消化管運動 / モチリン / 部位差 / 動物種差 / ghrelin / food intake / ghrelin受容体 / D-Lys3-GHRP-6 / 脳室内適用 / GHRP-6
Research Abstract

Background and aim. Ghrelin is the endogenous ligand for the growth hormone secretagogue receptor (GHS-R), and stimulates food intake and gastrointestinal (GI) motility in mammals. In chickens, on the other hand, ghrelin inhibits food intake. This discrepancy prompted us to investigate whether ghrelin affects gastrointestinal motility in the chicken. Methods, The contractile effect of chicken ghrelin was measured isometrically in muscle strips from different regions of the GI tract suspended in an organ bath. The effect of chicken ghrelin was compared with that of rat or human ghrelin. In addition, mRNA expression of ghrelin and GHS-R was examined by quantitative RT-PCR.
Results. Among the ghrelin peptides tested (rat, human and chicken ghrelin), only chicken ghrelin caused contraction of muscle strips from the GI tract. No effect was seen in des-octanoylated chicken ghrelin, suggesting that octanoylation at Ser^3 residue was essential for its activity. The contractile response to chick … More en ghrelin in the crop was not affected by tetrodotoxin (TTX), but that in the proventriculus was decreased by TTX and atropine to the same extent. D-Lys^3-GHRP-6 (a GHS-R antagonist) caused a transient contraction and inhibited the chicken ghrelin-induced contraction without affecting the 50 mM high-K^+-induced contraction. The amplitude of the chicken ghrelin-induced contraction was different among GI regions: highest in the crop and colon (70% of high-K^+-induced contraction), moderate in the esophagus and proventriculus (30%), and weak in the small intestine (10-20%) and the ranking order was consistent with GHS-R mRNA expression in each region (colon > crop = esophagus > proventriculus > duodenum =jejunum > ileum). GHS-R mRNA expression in the muscle preparations of crop and proventriculus without mucosa was 2-4 times higher than that in whole segments of crop and proventriculus, indicating that the GHS-R is mainly expressed in smooth muscle layers including enteric nerves. In contrast to the GHS-R expression, ghrelin mRNA was mainly expressed in the mucosa of proventriculus and duodenum.
Conclusion. These results indicate that ghrelin stimulates GI contractility and preferentially affects the motor activity of esophagus, crop and colon through myogenic and/or neurogenic mechanisms. These actions are mediated through the GHS-R, which is highly sensitive to chicken ghrelin Less

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (15 results)

All 2007 2006 2005 2004

All Journal Article (15 results)

  • [Journal Article] Contractile effects of ghrelin-related peptides on the chicken gastrointestinal tract in vitro.2007

    • Author(s)
      Kitazawa T, Kaiya H, Taneike T
    • Journal Title

      Peptides 28

      Pages: 617-624

    • NAID

      120005761137

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Contractile effects of ghrelin-related peptides on the chicken gastrointestinal tract in vitro.2007

    • Author(s)
      Kitazawa T, Kaiya H, Taneike T.
    • Journal Title

      Peptides 28

      Pages: 617-624

    • NAID

      120005761137

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Contractile effects of ghrelin-related peptides on the chicken gastrointestinal tract in vitro2007

    • Author(s)
      Kitazawa T, Kaiya H, Taneike T
    • Journal Title

      Peptides 28

      Pages: 617-624

    • NAID

      120005761137

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Intracerebroventricular administration of growth hormone releasing peptide-6 (GHRP-6) inhibits food intake, but not food retention of crop and stomach in neonatal chicks.2006

    • Author(s)
      Islam Kahan S, Doda K, Yahata K, Nishimoto S, Ueda H, Taneike T, Kitazawa T, Hosaka T, Bungo T
    • Journal Title

      The Journal of Poultry Sciences 43

      Pages: 35-40

    • NAID

      10017158484

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Pharmacological characterization of 5-hydroxytryptamine-induced contraction in chicken gastrointestinal tracts.2006

    • Author(s)
      Kitazawa T, Ukai H, Komori S, Taneike T
    • Journal Title

      Autonomic and Autacoid Pharmacology 26(2)

      Pages: 157-168

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Intracerebroventricular administration of growth hormone releasing peptide-6 (GHRP-6) inhibits food intake, but not food retention of crop and stomach in neonatal chicks.2006

    • Author(s)
      Islam Kahan S, Doda K, Yahata K, Nishimoto S, Ueda H, Taneike T, Kitazawa T, Hosaka T, Bungo T.
    • Journal Title

      The Journal of Poultry Sciences. 43

      Pages: 35-40

    • NAID

      10017158484

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Pharmacological characterization of 5-hydroxytryptamine-induced contraction in chicken gastrointestinal tracts.2006

    • Author(s)
      Kitazawa T, Ukai H, Komori S, Taneike T.
    • Journal Title

      Autonomic and Autacoid Pharmacol. 26(2)

      Pages: 157-168

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Gastrointestinal-motility stimulating action of ghrelin in isolated chicken gastrointestinal tracts2006

    • Author(s)
      Kitazawa T, Taneike T
    • Journal Title

      Acta Pharmagologica Sinica (Abstracts of the 15^th World Congress of Pharmacology) Supplement 1

      Pages: 291-291

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effects of ghrelin peptides on contractility of isolated chicken gastrointestinal tracts2006

    • Author(s)
      Kitazawa T, Taneike T
    • Journal Title

      J.Pharmacological Sciences 100 Supplement 1

      Pages: 165-165

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Pharmacological characterization of 5-hydroxytryptamine-induced contraction in the2006

    • Author(s)
      Kitazawa T, Ukai H, Komori S, Taneike T
    • Journal Title

      Autonomic and Autacoid Pharmacology 26(2)

      Pages: 157-168

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Intracerebroventricular administration of growth hormone releasing peptide-6 (GHRP-6) inhibits food intake but not food retention of crop and stomach in neonatal chicks2006

    • Author(s)
      Islam khan MS, Dodo K, Yahata K, Nishimoto S, Ueda H, Taneike T, Kitazawa T, Hosaka Y, Bungo T.
    • Journal Title

      J.Poultry Sciences 43(1)

      Pages: 35-40

    • NAID

      10017158484

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Gastric motor effects of peptide and non-peptide ghrelin agonists in mice in vivo and in vitro.2005

    • Author(s)
      Kitazawa T, De Smet B, Verbeke, K., Depoortere I., Peeters T.
    • Journal Title

      Gut 54(8)

      Pages: 1078-1084

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Gastric motor effects of peptide and non-peptide ghrelin agonists in mice in vivo and in vitro.2005

    • Author(s)
      Kitazawa T, De Smet B, Verbeke, K., Depoortere I., Peeters T.
    • Journal Title

      Gut. 54(8)

      Pages: 1078-1084

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Intracerebroventricular administration of chicken motilin doesnot induce hyperphargia in meat-type chicks2004

    • Author(s)
      Bungo T, Ueda H, Kitazawa T, Taneike T, Furuse M
    • Journal Title

      Physiol. Behavior 82

      Pages: 199-203

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Intracerebroventricular administration of chicken motilin does not induce hyperphargia in meat-type chicks.2004

    • Author(s)
      Bungo T, Ueda H, Kitazawa T, Taneike T, Furuse M.
    • Journal Title

      Physiol. Behavior 82

      Pages: 199-203

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi