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Analysis of mast cell activation mechanism in innate immunity

Research Project

Project/Area Number 16580248
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionAzabu University

Principal Investigator

IKEDA Teruo  Azabu university, Veterinary medicine, Associate professor, 獣医学部, 助教授 (60151297)

Co-Investigator(Kenkyū-buntansha) FUNABA Masayuki  Azabu university, Veterinary medicine, Associate professor, 獣医学部, 助教授 (40238655)
MURAKAMI Masaru  Azabu university, Veterinary medicine, Professor, 獣医学部, 教授 (80271360)
SHIROTA Kinji  Azabu university, Veterinary medicine, Professor, 獣医学部, 教授 (70147974)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsmast cell / Bone marrow derived mast cell / lipopolysaccharide / innate immunity / peptidoglycan / cytokine / DNA array / mmp / FimH / 大腸菌
Research Abstract

In this study we evaluated 2 subjects, 1. involvement of TGF-b family molecular in mast cell growth, differentiation and functions and 2. activation of mast cell in innate immunity. Our results show as follows:
1. Involvement of TGF-b family molecular in mast cell growth, differentiation and functions
1) Degranulation in mast cell is regulated by calmodulin pathway.
2) Expression of activin A in mast cell is regulated due to calcium.
3) Mouse mast cell protease-9 is transcriptionaly activated by microphthalimia-associated transcription factor.
4) Activin A in murine mast cells is modulated growth, differentiation, and migration.
5) Mouse mast cell protease-7 is transcriptionally activated of by activin and transforming growth factor-b is inhibited by microphthalmia-associated transcription factor.
6) Mouse mast cell protease-6 gene is up-regulated by transforming growth factor-b and activin in mast cell progenitors.
7) Mouse mast cell protease-7 is transcriptionally regulated by TGF-b.
8) Tocopherol-associated protein(TAP) is identified as an activin/TGF-b-inducible gene in mast cells.
9) Smad3 is required for mast cell growth.
10) Copulanar PCB enhance expression of TGF-b mRNA in mast cell.
2. Activation of mast cell in innate immunity.
1) Lipopolysaccharide and peptidoglycan alter murine mast cells functions.
2) Reliability of RT-PCR methods for measuring relative gene expression in mast cell.
3) Mast cell express tocopherol-associated protein.
4) Contribution of mast cells to bacterial clearance and their proliferation during experimental cyctitis induced by type 1 fimbriated E.coli.
5) Respoises during cell preparation for functional analyses in mouse bone marrow-derived cultured mase cells.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (28 results)

All 2006 2005 2004 2003

All Journal Article (27 results) Book (1 results)

  • [Journal Article] Involvement of p38 MAP kinase and Smad3 in TGF-beta-mediated mast cell functions.2006

    • Author(s)
      FUNABA M, IKEDA T, MURAKAMI M, OGAWA K, NISHINO Y, TSUCHIDA K, SUGINO H, ABE M
    • Journal Title

      Cell Signal. 18(12)

      Pages: 2154-2161

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Transcriptional regulation of mouse mast cell protease-7 by TGF-beta.2006

    • Author(s)
      FUNABA M, IKEDA T, MURAKAMI M, OGAWA K, NISHINO Y, TSUCHIDA K, SUGINO H, ABE M
    • Journal Title

      Biochim Biophys Acta. 1759

      Pages: 166-170

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Requirement of Smad3 for mast cell growth.2006

    • Author(s)
      FUNABA M, NAKAYA K, IKEDA T, MURAKAMI M, TSUCHIDA K, SUGINO H
    • Journal Title

      Cell Immunol. 240(1)

      Pages: 47-52

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification of tocopherol-associated protein as an activin/TGF-beta-inducible gene in mast cells.2006

    • Author(s)
      FUNABA M, MURAKAMI M, IKEDA T, OGAWA K, TSUCHIDA K, SUGINO H
    • Journal Title

      Biochim Biophys Acta. 1763(8)

      Pages: 900-906

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Transcriptional regulation of plasminogen activator inhibitor-1 by transforming growth factor-beta, activin A and microphthalmia-associated transcription factor.2006

    • Author(s)
      MURAKAMI M, IKEDA T, SAITO T, OGAWA K, NISHINO Y, NAKAYA K, FUNABA M
    • Journal Title

      Cell Signal. 18(2)

      Pages: 256-265

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Involvement of p38 MAP kinase and Smad3 in TGF-beta-mediated mast cell functions.2006

    • Author(s)
      FUNABA M, IKEDA T, MURAKAMI M, OGAWA K, NISHINO Y, TSUCHIDA K, SUGINO H, ABE M.
    • Journal Title

      Cell Signal. 18(12)

      Pages: 2154-61

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Transcriptional regulation of mouse mast cell protease-7 by TGF-beta.2006

    • Author(s)
      FUNABA M, IKEDA T, MURAKAMI M, OGAWA K, NISHINO Y, TSUCHIDA K, SUGINO H, ABE M.
    • Journal Title

      Biochim Biophys Acta. 1759

      Pages: 166-70

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Requirement of Smad3 for mast cell growth.2006

    • Author(s)
      FUNABA M, NAKAYA K, IKEDA T, MURAKAMI M, TSUCHIDA K, SUGINO H.
    • Journal Title

      Cell Immunol. 240(1)

      Pages: 47-52

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Identification of tocopherol-associated protein as an activin /TGF-beta-inducible gene in mast cells.2006

    • Author(s)
      FUNABA M, MURAKAMI M, IKEDA T, OGAWA K, TSUCHIDA K, SUGINO H.
    • Journal Title

      Biochim Biophys Acta. 1763(8)

      Pages: 900-6

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Transcriptional regulation of plasminogen activator inhibitor-1 by transforming growth factor-beta, activin A and microphthalmia-associated transcription factor.2006

    • Author(s)
      MURAKAMI M, IKEDA T, SAITO T, OGAWA K, NISHINO Y, NAKAYA K, FUNABA M.
    • Journal Title

      Cell Signal. 18(2)

      Pages: 256-65

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Transcriptional regulation of plaminogen activator inhibitor-1 by transformin growth factor-beta, activin A and microphthalmia-associated transcription factor.2006

    • Author(s)
      MURAKAMI M, IKEDA T, SAITO T, OGAWA K, NISHINO Y, NAKAYA K, FUNABA M.
    • Journal Title

      Cell Signal. 18(2)

      Pages: 256-65

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Transcriptional regulation of plasminogen activator inhibitor-1 by transforming growth factor-beta, activin A and microphthalmia-associated transcription factor.2006

    • Author(s)
      Murakami M, Ikeda T, Saito T, Ogawa K, Nishino Y, Nakaya K, Funaba M.
    • Journal Title

      Cell Signal. Feb;18(2)

      Pages: 256-265

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Responses during cell preparation for functional analyses in mouse bone marrow-derived cultured mast cells.2005

    • Author(s)
      MURAKAMI M, IKEDA T, NISHINO Y, FUNABA M
    • Journal Title

      Cell Immunol. 238(1)

      Pages: 49-55

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Up-regulation of mouse mast cell protease-6 gene by transforming growth factor-beta and activin in mast cell progenitors.2005

    • Author(s)
      Funaba M, Ikeda T, Murakami M, Ogawa K, Abe M
    • Journal Title

      Cell Signal. Jan;17(1)

      Pages: 121-128

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Responses during cell preparation for functional analyses in mouse bone marrow-derived cultured mast cells.2005

    • Author(s)
      MURAKAMI M, IKEDA T, NISHINO Y, FUNABA M.
    • Journal Title

      Cell Immunol. 238(1)

      Pages: 49-55

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Up-regulation of mouse mast cell protease-6 gene by transforming growth factor-beta and activin in mast cell progenitors.2005

    • Author(s)
      Funaba M, Ikeda T, Murakami M, Ogawa K, Abe M
    • Journal Title

      Cell Signal. 17(1)

      Pages: 121-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Responses during cell preparation for functional analyses in mouse bone marrow-derived cultured mast cells.2005

    • Author(s)
      Murakami M, Ikeda T, Nishino Y, Funaba M.
    • Journal Title

      Cell Immunol. Feb 11

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Up-regulation of mouse mast cell protease-6 gene by transforming growth factor-beta and activin in mast cell progenitors.2005

    • Author(s)
      Funaba M, Ikeda T, Murakami M, Ogawa K, Abe M.
    • Journal Title

      Cell Signal 17(1)

      Pages: 121-128

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression of tocopherol-associated protein in mast cells.2004

    • Author(s)
      Ikeda T, Murakami M, Funaba M
    • Journal Title

      Clin Diagn Lab Immunol. 11 (6)

      Pages: 1189-1191

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Reliability of RT-PCR methods for measuring relative gene expression in mast cells.2004

    • Author(s)
      Ikeda T, Murakami M, Funaba M
    • Journal Title

      Vet Immunol Immunopathol 100(1-2)

      Pages: 1-5

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of tocopherol-associated protein in mast cells.2004

    • Author(s)
      Ikeda T, Murakami M, Funaba M.
    • Journal Title

      Clin Diagn Lab Immunol. 11 (6)

      Pages: 1189-1191

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Reliability of RT-PCR methods for measuring relative gene expression in mast cells.2004

    • Author(s)
      Ikeda T, Murakami M, Funaba M.
    • Journal Title

      Vet Immunol Immunopathol. 100 (1-2)

      Pages: 1-5

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of tocopherol-associated protein in mast cells.2004

    • Author(s)
      Ikeda T, Murakami M, Funaba M.
    • Journal Title

      Clin Diagn Lab Immunol. 11(6)

      Pages: 1189-1191

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Reliability of RT-PCR methods for measuring relative gene expression in mast cells.2004

    • Author(s)
      Ikeda T, Murakami M, Funaba M.
    • Journal Title

      Vet Immunol Immunopathol 100(1-2)

      Pages: 1-5

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Contribution of mast cells to bacterial clearance and its proliferation during ecperimental cystitis induced by type 1 fimbriated E. coli.2003

    • Author(s)
      Malaviya, R., Ikeda, T., Abraham, SN., Malaviya, R.
    • Journal Title

      Immunol Lett. 91(2-3)

      Pages: 103-111

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Transcriptional activation of mouse mast cell Protease-7 by activin and transforming growth factor-beta is inhibited by microphthalmia-associated transcription factor.2003

    • Author(s)
      Funaba M, Ikeda T, Murakami M, Ogawa K, Tsuchida K, Sugino H, Abe M
    • Journal Title

      J Biol Chem. 278(52)

      Pages: 52032-52041

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Altered function of murine mast cells in response to lipopolysaccharide and peptidoglycan.2003

    • Author(s)
      Ikeda T, Funaba M.
    • Journal Title

      Immunol Lett. 88(1)

      Pages: 21-26

    • Related Report
      2004 Annual Research Report
  • [Book] 獣医臨床シリーズ2004年度版『エマージング感染症、リエマージング感染症』2005

    • Author(s)
      監訳:池田輝雄
    • Total Pages
      231
    • Publisher
      学窓社、東京
    • Related Report
      2005 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

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