| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Research Abstract |
This work investigated the isolation and characterization of the matrix polysaccharides in cell walls from the healthy leaf, gall, and callus of Distylium racemosum. The leaf cell walls consisted mainly of arabinose, xylose, glucose, and uronic acids, whereas the gall and callus cell walls consisted of arabinose, xylose, galactose, and uronic acids. Pectin solubilized from leaf, gall, and callus cell walls accounted for 3%, 7%, and 16%, respectively, of cell wall dry weight, whereas hemicellulose was solubilized 24% from depectinated cell walls of gall and callus. When the matrix polysaccharides were fractionated by anion-exchange chromatography, the hemicellulosic polymers of the gall and callus cell walls exhibited distinctly different elution patterns, compared with the leaf cell walls. A considerable difference in hemicellulose of the gall and callus cell walls as compared with leaf cell walls could be a consequence of a defect in arabinoxylan of cell wall structure.
Further many of the glycosyl-hydrolase activities were detected in the protein fractions solubilized with the buffer and the strong saline solution from leaf, gall, and callus. The β-galactosidase was purified from a protein fraction soluble in the buffer of callus cells and the purified enzyme was characterized with respect to the enzymatic properties such as molecular mass, isoelectric point, pH profile of activity and stability, temperature profile of activity and stability, inhibitors, and kinetic constant.
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