Construction of super-stable enzymes : distinct reversibility of structure and its mechanism
| Project/Area Number |
16580280
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kagoshima University |
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Principal Investigator |
TOKUNAGA Masao Kagoshima University, Faculty of Agriculture, Professor (20112782)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIBASHI Matsujiro Kagoshima University, Faculty of Agriculture, Associate Professor (20305163)
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| Project Period (FY) |
2004 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,950,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | halophilic enzyme / halophilic bacteria / nucleoside dinhosnhate kinase / chimeric protein / solubility / 好塩性 / 酸性アミノ酸 / 変異 / サブユニット / 蛋白質 / 高次構造形成 / フォールディング / キメラ蛋白質 |
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| Research Abstract |
(1) We have succeeded in the expression of haloarchaeal nucleoside diphosphate in Escherichia coil and X-ray structure analysis. The structure of main chain of polypeptide is quite similar to non-halophilic counterpart, indicating elegant distribution of side chains of acidic amino acid residues.
(2) We have cloned nucleoside diphosphate kinase gene from moderately halophilic bacteria and expressed it in E. coil. We found for the first time it forms dimeric structure.
(3) We have succeeded in the construction, expression, purification and characterization of chimeric nucleoside diphosphate kinase molecules from the enzymes isolated from Halomonas and Pseudomonas, which is halophilic and non-halophilic bacteria, respectively.
Consequently, the landmark of halophilic protein is found to be
(1) higher optimum salt concentration for enzymatic activity
(2) higher stability at higher salt environment
(3) highly reversible characteristics due to its high solubility
(4) slow mobility on SDS-polyacrylamide gel electrophoresis.
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Report
(5 results)
Research Products
(11 results)
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[Journal Article] Residue 134 detrmines the dimer-tetramer assembly of nucleoside diphosphate kinase from moderately halophilic bacteria2008
Author(s)
Tokunaga H., Ishibashi, M., Arisaka, F., Arai, S., Kuroki, R., Arakawa, T., Tokunaga, M.
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Journal Title
FEBS Lett 582
Pages: 1049-1054
Related Report
Peer Reviewed
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