• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Structural Design of Micro-and Nano-gel Particles for Appropriate Controlled Release of Peptide Drugs

Research Project

Project/Area Number 16590038
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionKobe Gakuin University

Principal Investigator

ICHIKAWA Hideki  Kobe Gakuin University, The Faculty of Pharmaceutical Sciences, Associate professor, 薬学部, 助教授 (00248105)

Co-Investigator(Kenkyū-buntansha) FUKUMORI Yoshinobu  Kobe Gakuin University, The Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (60102927)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsMicrocapsule / Controlled release / Peptide drug / Hydrogel / Coating / Nanoparticle / Stimuli-sensitive polymer / Hydroxypropyl cellulose / キトサン
Research Abstract

The aim of the present research project is to develop nano-and micro-particulate systems with appropriate controlled release functions for peptide drug delivery. The proposed systems are directed to long-term delayed release for peroral colon-specific peptide delivery, externally temperature-activated periodically pulsatile release for chronopharmacological peptide delivery, and pH-dependent prolonged release using thermo-and pH-sensitive nanogels for oral peptide delivery. The results obtained are summarized as follows :
1. The microcapsules composed of a lactose core, an insulin-layer and a coat of newly synthesized acrylic terpolymer made it possible to achieve a pH-independent delayed-release characterized by a lag-time and subsequent rapid insulin release. The microencapsulated insulin was found to be stable even after the storage at 4℃ for 24 months. In vivo oral administration studies of the microcapsules with a lag-time of 6h revealed a significant hypoglycemic effect, indicatin … More g insulin absorption through the colon. The pharmacological availability reached 5.1%.
2. The microcapsules having an ability to thermosensitively control the drug-release could be achieved by fabricating an ethylcellulose matrix coat containing nano-sized p(NIPAAm) hydrogels onto a drug-layered core. Introduction of poly(□-caprolactone) macromer into p(NIPAAm) nanogels or use of hydroxypropylcellulose as an alternative thermosensitively component was useful to achieve distinct thermosensitive periodical pulsatile release.
3. The nanogels composed of thermosensitive p(NIPAAm) core and pH sensitive (MAA-g-EG) shells (CSNPs) for oral peptide delivery were characterized in terms of drug loading capacity and muco-adhesive properties. Newly established drug-loading method was useful to significantly increase drug content (20 wt%). Atomic force microscopy (AFM) using colloidal probe of CSNPs for analyzing particle-mucin layer interaction could also be established : pH-dependent changes of interaction force were observed between CSNPs and mucin layer.
4. As a solvent-free preparation process, dry-powder coating technique could be established. This technology is expected to process moisture-sensitive peptide-drug stably in an aseptic condition. Less

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (29 results)

All 2007 2006 2005 2004

All Journal Article (24 results) Book (5 results)

  • [Journal Article] ナノハイドロゲル粒子を利用したペプチドの特殊放出制御型マイクロカプセルの設計2007

    • Author(s)
      市川秀喜, 福森義信
    • Journal Title

      薬学雑誌 127

      Pages: 813-823

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 医用応用 : マイクロカプセルのDDSへの応用2007

    • Author(s)
      市川秀喜, 福森義信
    • Journal Title

      化学工学 71・2

      Pages: 131-134

    • NAID

      10018543730

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Design of nanohydrogel-incorporated microcapsules for appropriate controlled-release of peptide drugs.2007

    • Author(s)
      Hideki Ichikawa, Yoshinobu Fukumori
    • Journal Title

      YAKUGAKU ZASSHI 127

      Pages: 813-823

    • NAID

      110006273926

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Medical application : Application of microcapsules to Drug Delivery Systems.2007

    • Author(s)
      Hideki Ichikawa, Yoshinobu Fukumori
    • Journal Title

      Chem. Eng. 71

      Pages: 131-134

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] マイクロカプセルのDSSへの応用2007

    • Author(s)
      市川秀喜, 福森義信
    • Journal Title

      化学工学 71(2)

      Pages: 131-134

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Short-term delayer-release microcapsules spraycoated with acrylic terpolymers2006

    • Author(s)
      D.Liu, H.Ichikawa, F.Cui, Y.Fukumori
    • Journal Title

      International Journal of Pharmaceutics 307

      Pages: 300-307

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Acrylic terpolymer microcapsules for colon-specific drug delivery : effect of molecular weight and solubility of microencapsulated drugs on their release behaviors2006

    • Author(s)
      M.Arimoto, Y.Fukumori, J.Fujiki, H.Ichikawa
    • Journal Title

      Journal of Drug Delivery Science and Technology 16

      Pages: 173-181

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Functional Stimuli-Responsive Nanogel-Particles for Oral Peptide Delivery : Preparation, Drug-Release Behaviors and In Vitro Cellular Interaction2006

    • Author(s)
      H.Ichikawa, Y.Fukumori, H.Kamiya
    • Journal Title

      Proceedings of NSTI Nanotech 2006 2

      Pages: 392-395

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Interaction between artificial mucin layer and stimuli-responsible nano-gel particles for the oral peptide delivery observed in simulated intestinal solutions by using colloid probe AFM method2006

    • Author(s)
      H.Kamiya, M.Yoshimura, M.Tsukada, H.Ichikawa, Y.Fukumori
    • Journal Title

      Proceedings of NSTI Nanotech 2006 2

      Pages: 404-407

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Short-term delayed-release microcapsules spraycoated with acrylic terpolymers.2006

    • Author(s)
      Dongchun Liu, Hideki Ichikawa, Fude Cui, Yoshinobu Fukumori
    • Journal Title

      Int. J. Pharm. 307

      Pages: 300-307

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Acrylic terpolymer microcapsules for colon-specific drug delivery : effect of molecular weight and solubility of microencapsulated drugs on their release behaviors.2006

    • Author(s)
      Masahiro Arimoto, Yoshinobu Fukumori, Junko Fujiki, Hideki Ichikawa
    • Journal Title

      J. Drug Del. Sci. Tech. 16

      Pages: 173-181

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Functional stimuli-responsive nanogel-particles for oral peptide delivery : Preparation, drug-release behaviors and in vitro cellular interaction.2006

    • Author(s)
      Hideki Ichikawa, Yoshinobu Fukumori, Hidehiro Kamiya
    • Journal Title

      Proceedings of NSTI-Nanotech Vol.2

      Pages: 392-395

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Interaction between artificial mucin layer and stimuli-responsive nanogel particles for the oral peptide delivery observed in simulated intestinal solutions by using colloid probe AFM method.2006

    • Author(s)
      Hidehiro Kamiya, Motoyasu Yoshimura, Mayumi Tsukada, Hideki Ichikawa, Yoshinobu Fukumori
    • Journal Title

      Proceedings of NSTI-Nanotech Vol.2

      Pages: 404-407

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Acrylic terpolymer microcapsules for colon-specific drug delivery : effect of molecular weight and solubility of microencapsulated drugs on their release behaviors2006

    • Author(s)
      M.Arimoto, Y.Fukumori, J.Fujiki, H.Ichikawa
    • Journal Title

      Journal of Drug Delivery Science and Technology 16(3)

      Pages: 173-181

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 医薬品製剤におけるナノテクノロジーの展開2006

    • Author(s)
      市川秀喜, 福森義信
    • Journal Title

      分離技術 36

      Pages: 359-364

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Acrylic terpolymer microcapsules for colon-specific drug delivery : effect of molecular weight and solubility of microencapsulated drugs on their release behaviors2006

    • Author(s)
      M.Arimoto, Y.Fukumori, J.Fujiki, H.Ichikawa
    • Journal Title

      Journal of Drug Delivery Science and Technology (印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 高分子ナノパウダーを用いた医薬品微粒子の乾式コーティング2006

    • Author(s)
      市川秀喜, 福森義信
    • Journal Title

      Pharm Tech Japan 22

      Pages: 119-124

    • Related Report
      2005 Annual Research Report
  • [Journal Article] ナノ粒子コーティングによる放出制御製剤の設計2005

    • Author(s)
      福森義信, 市川秀喜
    • Journal Title

      粉体工学会誌 42

      Pages: 573-581

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Design of controlled release pharmaceuticals in nanoparticle coating process.2005

    • Author(s)
      Yoshinobu Fukumori, Hideki Ichikawa
    • Journal Title

      J. Soc. Powder Technol, Japan 42

      Pages: 573-581

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] ハイドロゲルナノ粒子およびそのミクロ粒子へのアッセンブリングを利用したDDS2005

    • Author(s)
      市川秀喜, 福森義信
    • Journal Title

      バイオインダストリー 4

      Pages: 32-40

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Microencapsulation of water-soluble macromolecules with acrylic terpolymers by the Wurster process for colon-specific drug delivery2004

    • Author(s)
      M.Arimoto, H.Ichikawa, Y.Fukumori
    • Journal Title

      Powder Technology 141

      Pages: 177-186

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] 医薬品のコーティング技術と流動層2004

    • Author(s)
      福森義信, 市川秀喜
    • Journal Title

      混相流 18・3

      Pages: 214-222

    • NAID

      10013587496

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Microencapsulation of water-soluble macromolecules with acrylic terpolymers by the Wurster process for colon-specific drug delivery.2004

    • Author(s)
      Masahiro Arimoto, Hideki Ichikawa, Yoshinobu Fukumori
    • Journal Title

      Powder Tech. 141

      Pages: 177-186

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Coating of Pharmaceuticals and fluidized bed.2004

    • Author(s)
      Yoshinobu Fukumori, Hideki Ichikawa
    • Journal Title

      Jpn. J. Multiphase Flow 18

      Pages: 214-222

    • NAID

      10013587496

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Powder Technology Handbook2006

    • Author(s)
      H.Ichikawa, Y.Fukumori
    • Total Pages
      889
    • Publisher
      Taylor & Francis
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] MML Series Volume 8, Smart Nanoparticles in Nanomedicine2006

    • Author(s)
      H.Ichikawa, Y.Fukumori
    • Total Pages
      417
    • Publisher
      Kentus Books
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Book] ナノパーティクル・テクノロジー・ハンドブック2006

    • Author(s)
      市川秀喜
    • Total Pages
      561
    • Publisher
      日刊工業新聞社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Book] Powder Technology Handbook2006

    • Author(s)
      H.Ichikawa, Y.Fukumori
    • Total Pages
      889
    • Publisher
      Taylor & Francis
    • Related Report
      2005 Annual Research Report
  • [Book] 医薬品製剤における最新剤形・処方設計ノウハウ・事例集2005

    • Author(s)
      市川秀喜, 福森義信
    • Total Pages
      416
    • Publisher
      技術情報協会
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi