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CO_2 gas transporter in plasma membrane which is involved in gas exchange

Research Project

Project/Area Number 16590053
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionKumamoto University

Principal Investigator

ISOHAMA Yoichiro  Kumamoto University, Graduate School of Pharmaceutical Sciences, Department of Chemico-Pharmacological Sciences, Associate Professor, 大学院・医学薬学研究部, 助教授 (10240920)

Co-Investigator(Kenkyū-buntansha) MIYATA Takeshi  Kumamoto University, Graduate School of Pharmaceutical Sciences, Department of Chemico-Pharmacological Sciences, Professor, 大学院・医学薬学研究部, 教授 (90040310)
HISATSUNE Akinori  Kumamoto University, Graduate School of Pharmaceutical Sciences, Department of Chemico-Pharmacological Sciences, Assistant Professor, 大学院・医学薬学研究部, 助手 (50347001)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2004: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsGas exchange / Aquaporin / Membrane permeability / Sp1 transcription factor / Subcellular distribution / レチノイン酸 / 一酸化窒素 / Aquaporin-5 / p38 MAP kinase / Sp3転写因子 / 肺胞上皮細胞 / CO_2ガス
Research Abstract

O_2 and CO_2 gas transport through plasma membrane is important to maintain normal respiration between alveolar space and blood flow. However, the mechanism of membrane gas transport has not been established. In our preliminary studies, we have found the plasma membrane CO_2 gas permeability of alveolar type I epithelial cell was unusually high, suggesting the cell has CO_2 gas transporter. Therefore, we first examined the mechanism that is involved in high CO_2 gas permeability of the cells. Primary cultured rat alveolar type II cells spontaneously differentiated into type I-like cells. We found the expression level of aquaporin-5 (AQP5) was considerably increased in this culture. Then we found that AQP5 expression in Xenopus oocytes significantly increased membrane CO_2 gas permeability. AQP5 mutants, that lack histidine or cysteine residues in its sequence, did not increased CO_2 gas permeability, suggesting the importance of these amino acids. We also examined the transcriptional regulation of AQP5 in alveolar epithelial cells, and found that Sp1 transcription factor positively and Sp3 negatively regulate AQP5 expression. In addition, we have found that retinoic acid increased AQP5 expression through the activation of Sp1. Finally, we found that nitric oxide inhibited AQP5 activity through S-nitrisylation. These findings are useful to consider the physiological regulation of gas exchange at alveolar-blood barrier.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (11 results)

All 2006 2005 2004

All Journal Article (10 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Neutrophil elastase induces IL-8 gene transcription and protein release through p38/NF-kB activation via EGFR transactivation in a lung epithelial cell line.2006

    • Author(s)
      Kuwahara I, Lillehoi EP, Lu W, Singh IS, Isohama Y, Miyata T, Kim KC
    • Journal Title

      Am J Physiol Lung Cell Ohysiol (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Neutrophil elastase induces IL-8 gene transcription and protein release through p38/NF-κB activation via EGFR transactivation in a lung epithelial cell line.2006

    • Author(s)
      Kuwahara I, Lillehoi EP, Lu W, Singh IS, Isohama Y, Miyata T, Kim KC
    • Journal Title

      Am J Physiol Lung Cell Physiol (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Neutrophil elastase induces IL-8 gene expression in lung epithelial cell2006

    • Author(s)
      Kuwahara I, Lillehoi EP, Hisatsune A, Lu W, Isohama Y., Miyata T, Kim KC
    • Journal Title

      Am J Physiol Lung Cell Physiol (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Lipopolysaccharide changes the subcellular distribution of aquaporin 5 and increases plasma membrane water permeability in mouse lung epithelial cells2005

    • Author(s)
      Ohinata A, Nagai K, Nomura J, Hashimoto K, Hisatsune A, Miyata T, Isohama Y
    • Journal Title

      Biochem Biophys Res Commun 326

      Pages: 521-526

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Neutrophil elastase stimulates MUC1 gene expression through increased Spl binding to the MUC1 promoter.2005

    • Author(s)
      Kuwahara I, Lillehoi EP, Hisatsune A, Lu W, Isohama T, Miyata T, Kim KC
    • Journal Title

      Am J Physiol Lung Cell Physiol 289

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Royal jelly has estrogenic effects in vitro and in vivo.2005

    • Author(s)
      Mishima S, Suzuki KM, Isohama Y, Kuratsu N, Araki Y, Inoue M, Miyata T
    • Journal Title

      J Ethonopharmacol 101

      Pages: 215-220

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Estrogenic effects of royal jelly2005

    • Author(s)
      Mishima S, Miyata T, Suzuki KM, Araki Y, Akao Y, Isohama Y
    • Journal Title

      J Trad Med 22

      Pages: 171-175

    • NAID

      110002555189

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Neutrophil elastase stimulates MUC1 gene expression through increased Sp1 binding to the MUC1 promoter.2005

    • Author(s)
      Kuwahara I, Lillehoi EP, Hisatsune A, Lu W, Isohama Y., Miyata T, Kim KC
    • Journal Title

      Am J Physiol Lung Cell Physiol 289

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Estrogenic effects of royal jelly.2005

    • Author(s)
      Mishima S, Miyata T, Suzuki KM, Araki Y, Akao Y, Isohama Y
    • Journal Title

      J Trad Med 22

      Pages: 171-175

    • NAID

      110002555189

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Lipopolysaccharide changes the subcellular distribution of aquaporin 5 and increases plasma membrane water permeability in mouse lung epithelial cells.2005

    • Author(s)
      Ohinata A, Nagai K, Nomura J, Hashimoto K, Hisatsune A, Miyata T, Isohama Y.
    • Journal Title

      Biochem Biophys Res Commun. 326

      Pages: 521-526

    • Related Report
      2005 Annual Research Report 2004 Annual Research Report
  • [Patent(Industrial Property Rights)] マンガンイオンを含むアクアポリン機能調節剤2004

    • Inventor(s)
      礒濱洋一郎, 宮田 健, 長井一史
    • Industrial Property Rights Holder
      (財)くまもとテクノ産業財団
    • Industrial Property Number
      2004-143549
    • Filing Date
      2004-05-13
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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