| Project/Area Number |
16590059
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Kitasato University |
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Principal Investigator |
NAKAGAWA Yasuhito Kitasato University, School of Pharmaceutical Sciences, Professor, 薬学部, 教授 (00119603)
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| Co-Investigator(Kenkyū-buntansha) |
IMAI Hirotaka Kitasato University, School of Pharmaceutical, Associate Professor, 薬学部, 助教授 (50255361)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | testis / apoptosis / glutathione peroxidase / embryo / 胚発生 / トランスジェニックマウス / グルタチオペルオキシダーゼ / 精子形成 / ethane dimethane sulfonate |
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| Research Abstract |
Condition of primary culture of fertilized ovum was established to realized the mechanism of embryonic lethal of knockout mouse of PHGPx (GPx4). Inner cell mass (ICM) and prominent expression of PHGPx were formed during 3days culture of wild type of embryo. On the other hand, growth of ICM was significantly suppressed in PHGPx-KO embryo and was disappeared during 7 days culture. We prepared PHGPx-hetero mouse which were introduced exogeous PHGPx gene. KO-PHGPx mouse was rescued by the introduction of PHGPx gene.
Low expression of PHGPx in sperm caused severe infertility of male. EDS induced infertility of mouse by the injury of testis. Injury of testis was more prominent in hetero mouse whose expression of PHGPx was almost half as compare to wild type of mouse.
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